Analiza transkryptów indukowanych pod wpływem CCC w liniach izogenicznych odmiany Bezostnaja z genami Rth

• Autorzy: Kowalczyk K. , Lesniowska-Nowak J. , Nowak M.

Warianty tytułu

EN

Analysis of transcripts induced using CCC in isogenic lines cv. Bezostnaja with Rht genes

• Języki publikacji: PL

Abstrakty

PL

Do ochrony roślin przed wyleganiem wykorzystuje się często regulatory wzrostu. Wiedza na temat mechanizmu reakcji roślin, szczególnie zawierających geny karłowatości, oraz stwierdzenie wpływu na ekspresję genów Rht może być bardzo przydatna w przyszłości. Celem pracy była analiza transkryptów indukowanych pod wpływem CCC w blisko izogenicznych liniach pszenicy zwyczajnej (Triticum aestivum L.) odmiany Bezostnaja z genami karłowatości w locus Rht-B1 z zastosowaniem metody RT-PCR. Wykazano, że zastosowanie starterów oligo(dT)18 było bardziej użyteczne aniżeli losowych starterów heksamerowych. Po traktowaniu chlorkiem chlormekwatu stwierdzono wzmocnienie wielu frakcji RNA, co świadczy o wpływie tego związku na ekspresję wielu genów. Zastosowanie starterów oligo(dT)18 oraz losowych heksamerów nie pozwoliło na dokładną charakterystykę zmian ekspresji genów karłowatości w badanych liniach izogenicznych.

EN

Height regulators are used in wheat crops to protect plants against lodging. Knowledge about mechanism of plant reaction, especially with dwarfing genes, and estimation of height regulator influence on the Rht genes expression could be very important in future. The aim of this paper was the determination of the CCC influence on transcript induction in Bezostnaja isogenic lines with dwarfing genes in the locus Rht-B1, using the RT-PCR method. It was showed that using the oligo(dT)18 primers was more useful in comparison with the random hexamer primers. After the chlormequat chloride treatment the enhancing of many RNA fractions, was obtained which indicated that CCC causes expression changes of many genes. Using the oligo(dT)18 and the random hexamer primers did not allow the determination changes of dwarfing genes expression in the studied isogenic lines.

Słowa kluczowe

PL

chlorek chloromekwatu; gen Rth; genetyka roslin; geny karlowatosci; linie izogeniczne; metoda RT-PCR; odmiany roslin; odpornosc na wyleganie; ograniczanie wzrostu; pszenica; pszenica Bezostnaja; regulatory wzrostu; transkrypty

EN

chlormequat chloride; Rht gene; plant genetics; dwarfing gene; isogenic line; reverse transcription polymerase chain reaction; plant cultivar; lodging resistance; growth reduction; wheat; Bezostnaja cultivar; growth regulator; transcript analysis

• Wydawca: -
• Czasopismo: Zeszyty Problemowe Postępów Nauk Rolniczych
• Rocznik: 2009
• Tom: 542
• Numer: 1

Twórcy

autor

Kowalczyk K.

• Instytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie, ul.Akademicka 15, 20-950 Lublin

autor

Lesniowska-Nowak J.

autor

Nowak M.

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