Growth suppression of human breast carcinoma stem cells by lipid peroxidation product 4-hydroxy-2-nonenal and hydroxyl radical-modified collagen

• Autorzy: Cipak A. , Mrakovcic L. , Ciz M. , Lojek A. , Mihaylova B. , Goshev I. , Jaganjac M. , Cindric M. , Sitic S. , Margaritoni M. , Waeg G. , Balic M. , Zarkovic N.
• Języki publikacji: EN

Abstrakty

EN

Breast cancer is a leading cause of mortality and morbidity in women, mostly due to high metastatic capacity of mammary carcinoma cells. It has been revealed recently that metastases of breast cancer comprise a fraction of specific stem-like cells, denoted as cancer stem cells (CSCs). Breast CSCs, expressing specific surface markers CD44+CD24-/lowESA+ usually disseminate in the bone marrow, being able to spread further and cause late metastases. The fundamental factor influencing the growth of CSCs is the microenvironment, especially the interaction of CSCs with extracellular matrix (ECM). The structure and function of ECM proteins, such as the dominating ECM protein collagen, is influenced not only by cancer cells but also by various cancer treatments. Since surgery, radio and chemotherapy are associated with oxidative stress we analyzed the growth of breast cancer CD44+CD24-/lowESA+ cell line SUM159 cultured on collagen matrix in vitro, using either native collagen or the one modified by hydroxyl radical. While native collagen supported the growth of CSCs, oxidatively modified one was not supportive. The SUM159 cell cultures were further exposed to a supraphysiological (35 µM) dose of the major bioactive lipid peroxidation product 4-hydroxynonenal (HNE), a well known as "second messenger of free radicals", which has a strong affinity to bind to proteins and acts as a cytotoxic or as growth regulating signaling molecule. Native collagen, but not oxidised, abolished cytotoxicity of HNE, while oxidized collagen did not reduce cytotoxicity of HNE at all. These preliminary findings indicate that beside direct cytotoxic effects of anticancer therapies consequential oxidative stress and lipid peroxidation modify the microenvironment of CSCs influencing oxidative homeostasis that could additionally act against cancer.

Słowa kluczowe

EN

growth suppression; man; breast cancer; lipid peroxidation product; 4-hydroxy-2-nonenal; hydroxyl radical-modified collagen; cancer stem cell; disease suppression; woman; mortality; homeostasis; collagen; extracellular matrix; morbidity

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2010
• Tom: 57
• Numer: 2
• Opis fizyczny: p.165-171,fig.,ref.

Twórcy

autor

Cipak A.

• Department of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia

autor

Mrakovcic L.

autor

Ciz M.

autor

Lojek A.

autor

Mihaylova B.

autor

Goshev I.

autor

Jaganjac M.

autor

Cindric M.

autor

Sitic S.

autor

Margaritoni M.

autor

Waeg G.

autor

Balic M.

autor

Zarkovic N.

Bibliografia

• Balic M (2009) Disseminated tumor cells as biomarkers for breast cancer. Biomark Med 3: 215-217. 
• Balic M, Lin H, Young L, Hawes D, Giuliano A, McNamara G, Datar RH, Cote RJ (2006) Most early disseminated cancer cells detected in bone marrow of breast cancer patients have a putative breast cancer stem cell phenotype. Clin Cancer Res 12: 5615-5621. 
• Borrirukwanit K, Lafleur MA, Mercuri FA, Blick T, Price JT, Fridman R, Pereira JJ, Leardkamonkarn V, Thompson EW (2007) The type I collagen induction of MT1-MMP-mediated MMP-2 activation is repressed by alphaVbeta3 integrin in human breast cancer cells. Matrix Biol 26: 291-305. 
• Borovic S, Sunjic I, Wildburger R, Mittelbach M, Waeg G, Zarkovic N (2007) Lipid peroxidation end product as a modulator of cell growth. In: Progress in cell growth process research. Hayashi T, ed, pp 187-208. Nova Science Publishers Inc, New York.
• Boyd NF, McGuire V (1991) The possible role of lipid peroxidation in breast cancer risk. Free Radic Biol Med 10: 185-190. 
• Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantitites of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254. 
• Braun S, Vogl FD, Naume B, Janni W, Osborne MP, Coombes RC, Schlimok G, Diel IJ, Gerber B, Gebauer G, Pierga JY, Marth C, Oruzio D, Wiedswang G, Solomayer EF, Kundt G, Strobl B, Fehm T, Wong GYC, Bliss J, Vincent-Salomon A, Pantel K (2005) A pooled analysis of bone marrow micrometastasis in breast cancer. N Engl J Med 353: 793-802. 
• Brown E, McKee T, diTomaso E, Pluen A, Seed B, Boucher Y, Jain RK (2003) Dynamic imaging of collagen and its modulation in tumors in vivo using second-harmonic generation. Nat Med 9: 796-800. 
• Cazacu M, Oniu T, Lungoci C, Mihailov A, Cipak A, Klinger R, Weiss T, Zarkovic N (2003) The influence of Isorel on the advanced colorectal cancer. Cancer Biother Radiopharm 18: 27-34. 
• Charafe-Jauffret E, Monville F, Ginestier C, Dontu G, Birnbaum D, Wicha MS (2008) Cancer stem cells in breast: current opinion and future challenges. Pathobiology 75: 75-84. 
• Ciz M, Cizova H, Pejchalova K, Jancinova V, Goshev I, Mihaylova B, Nosal R, Lojek A (2009) Interactions of oxidatively modified calf skin collagen with platelets and phagocytes. Neuroendocrinol Lett 30: 128-132. 
• Condell RA, Sakai N, Mercado RT, Larenas E (1988) Quantitation of collagen fragments and gelatin by deconvolution of polarimetry denaturation curves. Coll Relat Res 8: 407-418. 
• Danielsen CC (1982) Precision method to determine denaturation temperature of collagen using ultraviolet difference spectroscopy. Coll Relat Res 2: 143-150. 
• Dontu G, Abdallah WM, Foley JM, Jackson KW, Clarke MF, Kawamura MJ, Wicha MS (2003) In vitro propagation and transcriptional profiling of human mammary stem/progenitor cells. Genes Dev 17: 1253-1270. 
• Esterbauer H, Schaur RJ, Zollner H (1991) Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radic Biol Med 11: 81-128. 
• Fillmore CM, Kuperwasser C (2008) Human breast cancer cell lines contain stem-like cells that self-renew, give rise to phenotypically diverse progeny and survive chemotherapy. Breast Cancer Res 10: R25. 
• Gudjonsson T, Ronnov-Jessen L, Villadsen R, Rank F, Bissell MJ, Petersen OW (2002) Normal and tumor-derived myoepithelial cells differ in their ability to interact with luminal breast epithelial cells for polarity and basement membrane deposition. J Cell Sci 115: 39-50. 
• Hadden J (1999) The immunology and immunotherapy of breast cancer: an update. Int J Immunopharmacol 21: 79-101. 
• Hajj M, Wicha MS, Benito-Hernandez A, Morrison SJ, Clarke MF (2003) Prospective identification of tumorigenic breast cancer cells. Proc Natl Acad Sci USA 100: 3983-3988. 
• Horenstein MS, Vander Heide RS, L'Ecuyer TJ (2000) Molecular basis of anthracycline-induced cardiotoxicity and its prevention. Mol Genet Metab 71: 436-444. 
• Imlay JA, Chin SM, Linn S (1988) Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro. Science 240: 640-642. 
• Ingber DE (2008) Can cancer be reversed by engineering the tumor microenvironment? Semin Cancer Biol 18: 356-364. 
• Jemal A, Siegel R, Ward E, Murray T, Xu J, Smigal C, Thun MJ (2006) Cancer statistics 2006. CA Cancer J Clin 56: 106-130. 
• Kökoğlu E, Karaarslan I, Karaarslan HM, Baloğlu H (1994) Alterations of serum lipids and lipoproteins in breast cancer. Cancer Lett 82: 175-178. 
• Komsa-Penkova R, Koynova R, Kostov G, Tenchov B (1999) Discrete reduction of type I collagen thermal stability upon oxidation. Biophys Chem 83: 185-195. 
• Kreuzer T, Grube R, Zarkovic N, Schaur RJ (1998) 4-Hydroxynonenal modifies the effects of serum growth factors on the expression of the c-fos proto-oncogene and the proliferation of HeLa carcinoma cells. Free Radic Biol Med 25: 42-49. 
• Li L, Xie T (2005) Stem cell niche: structure and function. Annu Rev Cell Dev Biol 21: 605-631.  
• Li XY, Ota I, Yana I, Sabeh F, Weiss SJ (2008) Molecular dissection of the structural machinery underlying the tissue-invasive activity of membrane type-1 matrix metalloproteinase. Mol Biol Cell 19: 3221-3233. 
• Miller EJ, Rhodes RK (1982) Preparation and characterization of the different types of collagen. Methods Enzymol 82 (Part A): 33-64. 
• Morrison BJ, Schmidt CW, Lakhani SR, Reynolds BA, Lopez JA (2008) Breast cancer stem cells: implications for therapy of breast cancer. Breast Cancer Res 10: 210. 
• Pinner S, Sahai E (2008) PDK1 regulates cancer cell motility by antagonising inhibition of ROCK1 by RhoE. Nat Cell Biol 10: 127-137. 
• Poli G, Schaur RJ (2000) 4-Hydroxynonenal in the pathomechanisms of oxidative stress. IUBMB Life 50: 315-321. 
• Ponti D, Costa A, Zaffaroni N, Pratesi G, Petrangolini G, Coradini D, Pilotti S, Pierotti MA, Daidone MG (2005) Isolation and in vitro propagation of tumorigenic breast cancer cells with stem/progenitor cell properties. Cancer Res 65: 5506-5511. 
• Privalov PL, Tiktopulo EI (1970) Thermal conformational transformation of tropocollagen. I. Calorimetric study. Biopolymers 9: 127-139. 
• Ray G, Husain SA (2001) Role of lipids, lipoproteins and vitamins in women with breast cancer. Clin Biochem 34: 71-76. 
• Sabeh F, Ota I, Holmbeck K, Birkedal-Hansen H, Soloway P, Balbin M, Lopez-Otin C, Shapiro S, Inada M, Krane S, Allen E, Chung D, Weiss SJ (2004) Tumor cell traffic through the extracellular matrix is controlled by the membrane-anchored collagenase MT1-MMP. J Cell Biol 167: 769-781. 
• Sabeh F, Shimizu-Hirota R, Weiss SJ (2009) Protease-dependent versus -independent cancer cell invasion programs: three-dimensional amoeboid movement revisited. J Cell Biol 185: 11-19. 
• Sayre LM, Zelasko DA, Harris PL, Perry G, Salomon RG, Smith MA (1997) 4-Hydroxynonenal-derived advanced lipid peroxidation end products are increased in Alzheimer's disease. J Neurochem 68: 2092-2097. 
• Schaur RJ, Dussing G, Kink E, Schauenstein E, Posh K, Kukovetz E, Egger G (1994) The lipid peroxidation product 4-hydroxynonenal is formed and is able to attract rat neutrophils in vivo. Free Radic Res 20: 365-373. 
• Selley ML, Bourne DJ, Bartlett MR,Tymms KE, Brook AS, Duffeld AM, Ardlie NG (1992) Occurrence of (E)-4-hydroxy-2-nonenal in plasma and synovial fluid of patients with rheumatoid arthritis and osteoarthritis. Ann Rheum Dis 51: 481-484. 
• Sirchia R, Luparello C (2005) Collagen-induced differential expression of an RNA polymerase subunit by breast cancer cells. Biochimie 87: 669-672. 
• Sovic A, Borovic S, Loncaric I, Kreuzer T, Zarkovic K, Vukovic T, Wäg G, Hrascan R, Wintersteiger R, Klinger R, Zurak N, Schaur RJ, Zarkovic N (2001) The carcinostatic and proapoptotic potential of 4-hydroxynonenal in HeLa cells is associated with its conjugation to cellular proteins. Anticancer Res 21: 1997-2004. 
• Spradling A, Drummond-Barbosa D, Kai T (2001) Stem cells find their niche. Nature 414: 98-104. 
• Toyokuni S, Okamoto K, Yodoi J, Hiai H (1995) Persistent oxidative stress in cancer. FEBS Lett 358: 1-3. 
• Tumbar T, Guasch G, Greco V, Blanpain C, Lowry W, Reudl M, Fuchs E (2004) Defining the epithelial stem cell niche in skin. Science 303: 359-363. 
• Uchida K (2003) 4-Hydroxy-2-nonenal: a product and mediator of oxidative stress. Prog Lipid Res 42: 318-343. 
• Uchida K, Szweda LI, Chae HZ, Stadtman ER (1993) Immunochemical detection of 4-hydroxynonenal protein adducts in oxidized hepatocytes. Proc Natl Acad Sci USA 90: 8742-8746. 
• Wallace DG, Condell RA, Donovan JW, Paivinen A, Rhee WM, Wade SB (1986) Multiple denaturational transitions in fibrillar collagen. Biopolymers 25: 1875-1895. 
• Wolf K, Mazo I, Leung H, Engelke K, von Andrian UH, Deryugina EI, Strongin AY, Bröcker EB, Friedl P (2003) Compensation mechanism in tumor cell migration: mesenchymal-amoeboid transition after blocking of pericellular proteolysis. J Cell Biol 160: 267-277. 
• Yoritaka A, Hattori N, Uchida K, Tanaka M, Stadtman ER, Mizuno Y (1996) Immunohistochemical detection of 4-hydroxynonenal protein adducts in Parkinson disease. Proc Natl Acad Sci USA 93: 2696-2701. 
• Zarkovic K (2003a) 4-Hydroxynonenal and neurodegenerative diseases. Mol Aspects Med 24: 293-303. 
• Zarkovic N (2003b) 4-Hydroxynonenal as a bioactive marker of pathopysiological processes. Mol Aspects Med 24: 281-291. 
• Zarkovic N, Ilic Z, Jurin M, Scahur RJ, Puhl H, Esterbauer H (1993) Stimulation of HeLa cell growth by physiological con­centrations of 4-hydroxynonenal. Cell Biochem Funct 11: 279-286. 
• Zarkovic N, Zarkovic K, Schaur RJ, Stolc S, Schlag G, Redl H, Waeg G, Borovic S, Loncaric I, Juric G, Hlavka V (1999) 4-Hydroxynonenal as a second messenger of free radicals and growth modifying factor. Life Sci 65: 1901-1904. 
• Zarkovic N, Cipak A, Cindric M, Waeg G, Borovic Sunjic S, Mrakovcic L, Jaganjac M, Kolenc D, Andrisic L, Gveric Ahmetasevic S, Katusic A, Cherkas A, Juric Sekhar G, Wildburger R, Zarkovic K (2009) 4-Hydroxynonenal-protein adducts as biomarkers of oxidative stress, lipid peroxidation and oxidative homeostasis. In Free radicals, health and lifestyle. Caporossi D, Pigozzi F, Sabatini S, eds, pp 37-45. Medimond Srl, Bologna, Italy.