Chitin-glucan complex production by Schizophyllum commune submerged cultivation

• Autorzy: Smirnou D. , Krcmar M. , Prochazkova E.
• Języki publikacji: EN

Abstrakty

EN

Chitin-glucan complex is a fungal origin copolymer that finds application in medicine and cosmetics. Traditionally, the mycelium of Micromycetes is considered as an industrial chitin-glucan complex source. Basidiomycete Schizophyllum commune submerged cultivation for chitin-glucan complex production was studied. In different S.commune strains chitin-glucan complex composed 15.2 ± 0.4 to 30.2 ± 0.2% of mycelium dry weight. Optimized conditions for chitin-glucan complex production (nutrient medium composition in g/l: sucrose – 35, yeast extract – 4, Na₂HPO₄ *12H₂O – 2.5, MgSO₄ *7 H₂O – 0.5; medium initial pH 6.5; aeration intensity 2 l of air per 1 l of medium; 144 hours of cultivation) resulted in 3.5 ± 0.3 g/l complex yield. Redirection of fungal metabolism from exopolysaccharide synthesis to chitin-glucan complex accumulation was achieved most efficiently by aeration intensity increase. Chitin-glucan complex from S.commune had the structure of microfibers with diameter 1–2 µm, had water-swelling capacity of 18 g/g, and was composed of 16.63% chitin and 83.37% glucan with a degree of chitin deacetylation of 26.9 %. S.commune submerged cultivation is a potent alternative to Micromycetes for industrial-scale chitin-glucan complex production.

Słowa kluczowe

EN

chitin-glucan complex; production; Schizophyllum commune; submerged cultivation; copolymer; fungal copolymer; Micromycetes; isopropanol; filamentous fungi; cell wall; mycelium; fungal metabolism; exopolysaccharide

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2011
• Tom: 60
• Numer: 3
• Opis fizyczny: p.223-228,fig.,ref.

Twórcy

autor

Smirnou D.

• CPN Ltd., Dolni Dobrouc 401, 561 02 Dolni Dobrouc, Czech Republic

autor

Krcmar M.

autor

Prochazkova E.

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