Biological control of tomato verticillium wilt disease by Talaromyces flavus

• Autorzy: Naraghi L. , Heydari A. , Rezaee S. , Razavi M. , Jahanifar H. , Khaledi E. M.

Warianty tytułu

PL

Biologiczne zwalczanie uwiądu pomidora przy pomocy grzyba Talaromyces flavus

• Języki publikacji: EN

Abstrakty

EN

In this study, Talaromyces flavus a fungal antagonist, was isolated from soil samples collected from tomato fields in Tehran and the Western Azarbayjan provinces of Iran.Antagonistic effects of T. flavus isolates against Verticillium albo-atrum (V. a.-a.), the causal agent of tomato wilt disease were investigated under laboratory and greenhouse conditions. Soil samples from tomato fields in the Varamin and Uremia regions of Tehran and the Western Azarbayjan provinces respectively, were cultured on selective medium. T.flavus colonies were recovered after three weeks. In the laboratory experiments, antagonistic effects of volatile and non-volatile extracts of T. flavus isolates on V. a.-a.growth were investigated. Among isolates, five that caused higher growth inhibition of V. a.-a. were selected for greenhouse experiments. In the greenhouse, first inoculum of V. a.-a. and treatments affected by T. flavus isolates were prepared. For comparison of the infection index in treatments, the greenhouse experiment wasperformed with a split plot arranged in a randomized complete block design with four replications. Result of greenhouse experiments on different types of T.flavus treatments indicated that there was no significant difference among them. However, among five T. flavus isolates, the most effective one was Tf-To-V-24 and Tf-To-U-36. In the experiment on the interaction between different T. flavus treatments and T. flavus isolates,a minimum infection index was observed when both soil and seed were treated with Tf-To-V-31. The overall results of this study show that it may be possible to manage tomato Verticillium wilt disease effectively by T. flavus.

PL

W pracy przedstawiono wyniki badań nad antagonistycznym grzybem Talaromyces flavus, wyizolowanym z próbek gleby zebranych z polowych upraw pomidora w Tehranie i zachodnim Azarbayjanie, prowincjach Iranu. W warunkach laboratoryjnych i szklarniowych, badano antagonistyczny wpływ izolatów T. flavus przeciwko czynnikowi sprawczemu uwiądu pomidorów – Verticillium albo-atrum (V. a.-a.). Próbki gleby zebrane w regionach Varamin i Uremia (Tehran) oraz zachodnich prowincjach Azarbayjanu, hodowano na pożywce selektywnej. Trzy tygodniowe kultury grzyba T. flavus wykorzystano do dalszych badań. W doświadczeniach laboratoryjnych badano antagonistyczny wpływ lotnych i nielotnych ekstraktów pochodzących z izolatów grzyba T. flavus na wzrost V. a.-a. Do badań szklarniowych wybrano pięć izolatów T. flavus znacznie ograniczających wzrost V. a.-a. Przed przystąpieniem do doświadczeń szklarniowych, przygotowano inokulat V. a.-a. oraz różne ekspozycje grzyba T. flavus. W celu porównania indeksu porażenia poszczególnych wariantów, doświadczenia szklarniowe przeprowadzono w na pojedynczo rozszczepionych poletkach w układzie bloków losowanych w czterech powtórzeniach. Wyniki badań szklarniowych, nie wykazały istotnej różnicy pomiędzy kombinacjami z wykorzystaniem grzyba T. flavus, choć spośród pięciu badanych izolatów T. flavus, najskuteczniejsze okazały się kombinacje Tf-To-V-24 i Tf-To-U-36. Badając interakcję w różnych kombinacjach z grzybem T. flavus, najniższy indeks porażenia stwierdzono zarówno w glebie, jak i nasionach traktowanych Tf-To-V-31. Wyniki badań wskazują na możliwość skutecznego ograniczania występowania uwiądu pomidora przy pomocy grzyba T. flavus.

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2010
• Tom: 50
• Numer: 3
• Opis fizyczny: p.360-365,fig.,ref.

Twórcy

autor

Naraghi L.

• Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, P.O. Box 14515/775, Tehran, Iran

autor

Heydari A.

autor

Rezaee S.

autor

Razavi M.

autor

Jahanifar H.

autor

Khaledi E. M.

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