Sympathetic innervation of vestigial uterus of the European bison bulls

• Autorzy: Skobowiat C. , Panasiewicz G. , Szafranska B. , Sidelnikow S. , Bieniek M. , Zamojska A. , Wardzynska M. , Gizejewski Z.
• Języki publikacji: EN

Abstrakty

EN

This study describes the expression of DßH as a marker of sympathetic (catecholaminergic) innervation in the vestigial uterus of adult European bison (Eb) bulls. Cryo-sectioned uteri were examined by fluorescent immunohistochemistry with the use of primary rabbit DßH polyclonal antibodies. The DßH-immunocomplexes (antigens/polyclonals) were visualised by secondary donkey antirabbit biotinylated IγG, and then with cyanine (CY™3)-conjugated streptavidin. The DßH immunodetection with CY™3 for the Eb uterine sections was performed in parallel to a positive control (porcine sympathetic paravertebral ganglion), and a negative control (without primary anti-DßH polyclonals). This is the first paper describing the identification of DßH expression within the vestigial uterus of the adult Eb bulls. The distribution pattern of DßH expression localised within immunoreactive (IR) uterine nerve fibres of the bulls resembled that in the control female uterus. The DßH-IR nerve fibres were identified in the entire cross-section of each uterus, with generally higher density in the myometrium than in the endometrium. The ratio of the endometrial/myometrial DßH-expression (percentage of area with IR-signals) was comparable in all males and females (0.78 and 0.64, respectively). However, the DßH-expression area (%) was significantly lower within the male uterine endometrial (P<0.01) and myometrial regions (P<0.001) comparing to the female counterparts. Presumably, the DßH-IR expression within the male Eb uterus is associated with a trophic effect of noradrenaline released by sympathetic nerve fibres influencing nutrient supply of this vestigial organ in the Eb bulls.

Słowa kluczowe

EN

sympathetic innervation; vestigial uterus; uterus; European bison; bull; dopamine beta-hydroxylase; nerve fibre; expression

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 3
• Opis fizyczny: p.415-418,fig.,ref.

Twórcy

autor

Skobowiat C.

• Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland

autor

Panasiewicz G.

autor

Szafranska B.

autor

Sidelnikow S.

autor

Bieniek M.

autor

Zamojska A.

autor

Wardzynska M.

autor

Gizejewski Z.

Bibliografia

• 1. Duda I.V.: Role of adrenergic mechanisms in the regulation of uterine contraction. Akush Ginekol (Moscow) 1984, 7, 6-9.
• 2. Gizejewski Z., Glogowski J., Bielecki W., Demianowicz W.: Characteristic of the vestigial masculine uterus of the European Bison (Bison bonasus L.) - Preliminary results. The 4th Symposium of the Society for Biology of Reproduction and Joint Polish-Japanese Seminary TBR, Book of Abstracts, 2005, pp. 203-204.
• 3. Josso N., Belville C., di Clemente N., Picard J.Y.: AMH and AMH receptor defects in persistent Müllerian duct syndrome. Hum Reprod Update 2005, 11, 351-356.
• 4. Kiewisz J., Melo de Sousa N., Beckers J.F., Panasiewicz G., Gizejewski Z., Szafrańska B.: Identification of multiple pregnancy-associated glycoproteins (PAGs) purified from the European bison (Eb; Bison bonasus L.) placentas. Anim Reprod Sci 2009, 112, 229-250.
• 5. Lakomy M., Kaleczyc J., Majewski M., Sienkiewicz W.: Peptidergic innervation of the bovine vagina and uterus. Acta Histochem 1995, 97, 53-66.
• 6. Leese H.J., Hugentobler S.A., Gray S.M., Morris D.G., Sturmey R.G., Whitear S.L., Sreenan J.M.: Female reproductive tract fluids: composition, mechanism of formation and potential role in the developmental origins of health and disease. Reprod Fertil Dev 2008, 20, 1-8.
• 7. Meier C., Partlow G., Fisher K., Rennie B.: Persistent paramesonephric ducts (masculine uterus) in the male North American Beaver (Castor Canadensis). Can J Zool 1998, 76, 1188-1193.
• 8. Panasiewicz G., Zamojska A., Wardzynska M., Bieniek M., Gizejewski Z., Szafranska B.: Morphology and protein diversity of vestigial uteri in bulls of the European bison (Bison bonasus L.). XLIIIth Symposium of the Polish Society for Histochemistry and Cytochemistry. Med Biol Sci 2009, 23 (supl. 2), P021, p. 28.
• 9. Pozor M.: Diagnostic applications of ultrasonography to stallion's reproductive tract. Theriogenology 2005, 64, 505-509.
• 10. Schulman J., Levine S.H.: Pyometra involving uterus masculinus in a cat. J Am Vet Med Assoc 1989, 194, 690- 691.
• 11. Shehata R.: Male uterus in the donkey and horse. Acta Anat (Basel) 1978, 101, 245-248.
• 12. Skobowiat C., Szafrańska B., Panasiewicz G., Majewski M., Majewska M., Gizejewski Z.: Expression of dopamine ß-hydroxylase (DßH) in uterus-originated nerve fibres surrounding the placentomes of the European bison (Bison bonasus L.). Bull Vet Inst Pulawy 2009, 53, 415-419.
• 13. Swiezynski K.: The male reproductive organs of the European bison. Acta Theriogenol 1968, 32, 511-551.
• 14. Szafrańska B., Panasiewicz G., Dabrowski M., Majewska M., Gizejewski Z., Beckers J.F.: Chorionic mRNA expression and N-glycodiversity of pregnancy-associated glycoprotein family (PAG) of the European bison (Bison bonasus). Anim Reprod Sci 2005, 88, 225-243.
• 15. Szafrańska B., Panasiewicz G., Majewska M., Dajnowiec J., Kuber K., Bociek K., Gizejewski Z., Goczkowski M.: Amplicon-polymorphism of the Pregnancy-Associated Glycoprotein gene family (PAG) examined in the European bison: the Swedish population and bulls with regressive uterus from Poland. XXVIIth Congress of the International Union of Game Biologists, Hannover, Germany, Book of Extended Abstracts 2005, pp. 246-248.
• 16. Szafranska B., Panasiewicz G., Majewska M.: Biodiversity of multiple Pregnancy-Associated Glycoprotein (PAG) family: gene cloning and chorionic protein purification in domestic and wild eutherians (Placentalia) - a review. Reprod Nutr Dev 2006, 5, 481-502.
• 17. Thomas S.A., Matsumoto A.M., Palmiter R.D.: Noradrenaline is essential for mouse foetal development. Nature 1995, 374, 643-646.
• 18. Thorbert G., Alm P., Owman C., Rosengren E., Sjoberg N.O.: Differential effect on the sympathetic transmitter level in uterus and other organs of guinea-pig by drugs interfering with adrenergic nerve functions. Acta Physiol Scand 1978, 104, 203-212.
• 19. Whyte A., Monteagudo L.V., Diaz-Otero A., Lebrero M.E., Tejedor M.T., Falceto M.V., Whyte J., Gallego M.: Malformations of the epididymis, incomplete regression of the mesonephric tubules and hyperplasia of Leydig cells in canine persistence of Müllerian duct syndrome. Anim Reprod Sci 2009, 115, 328-333.
• 20. Witschi E.: Embryology of the uterus: normal and experimental. Ann NY Acad Sci 1959, 75, 412-435.