Microsatellite polymorphisms in the ovine prion protein gene in relation to PRP genotypes

• Autorzy: Wisniewska E. , Piwczynski D. , Mroczkowski S.
• Języki publikacji: EN

Abstrakty

EN

The aims of the study were to detect the polymorphisms of three microsatellite sites: S11, S15, and S24 in the ovine PRNP and to asses their relationship with prion protein (PrP) genotypes in three sheep breeds. To identify 15 PrP genotypes based on polymorphisms at codons 136, 154, and 171, PCR-RFLP analyses were applied. The microsatellite sites were amplified. For each microsatellite two or three alleles per site in homo- and heterozygotic combinations were observed. In the group of scrapie resistant sheep (NSP1), the genotype 152/152 bp of the S11 microsatellite, 179/179 bp of S15, and 144/144 bp of S24 occurred with the highest frequencies. The results of Fisher's exact test showed that associations between PrP genotypes and microsatellite alleles and genotypes in the PRNP were statistically significant (P<0,001). In conclusion, the investigated microsatellite sites may be used as additional genetic markers in prion protein gene variability studies in sheep.

Słowa kluczowe

EN

microsatellite; polymorphism; prion protein gene; sheep; genotype; scrapie; goat; animal disease; transmissible spongiform encephalopathy

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 4
• Opis fizyczny: p.705-711,ref.

Twórcy

autor

Wisniewska E.

• Department of Genetics and General Animal Breeding, University of Technology and Life Sciences in Bydgoszcz, 85-084 Bydgoszcz, Poland

autor

Piwczynski D.

autor

Mroczkowski S.

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