Effect of zearalenone on steroid secretion by porcine follicular cells in mono- and coculture

• Autorzy: Jakimiuk E. , Gajecka M. , Jana B. , Obremski K. , Gajecki M.
• Języki publikacji: EN

Abstrakty

EN

The objective of the study was to evaluate the impact of zearalenone (ZEA) on the production and secretion of some steroid sex hormones by granulosa cells in monoculture and granulosa cells with theca interna cells in coculture. Follicular cells were obtained from ovarian follicles of pre-pubertal gilts. They were exposed to 0.2% DMSO and ZEA (at a concentration of 0.4, 4, 40, and 400 ng/mL), for 48 h. The concentration of progesterone, testosterone, and oestradiol-17ß in the medium was determined by radioimmunoassay. It was found that both stimulating and inhibitory effect of ZEA on basal steroidogenesis in porcine ovarian follicular cells was depended on the type of cultures and concentrations of mycotoxin.

Słowa kluczowe

EN

coculture; follicular cell; monoculture; pig; prepubertal gilt; sex hormone; steroid hormone; steroid secretion; zearalenone; granulosa cell; progesterone; testosterone; 17-beta estradiol; radioimmunoassay; steroidogenesis; mycotoxin

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 3
• Opis fizyczny: p.419-423,fig.,ref.

Twórcy

autor

Jakimiuk E.

• Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland

autor

Gajecka M.

autor

Jana B.

autor

Obremski K.

autor

Gajecki M.

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