Antagonistic properties of bacteria found in sugar beet leaf silage against pathogens naturally occurring in the mouth of cattle

• Autorzy: Galbas M. , Dullin P. , Selwet M.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to present antibacterial properties of bacteria found in sugar beet silage against Shigella. The experiment involved bullocks, from which the pathogenic bacteria were isolated, and microorganisms obtained from silage (without additives). It was found that pathogenic bacteria are inhibited by bacteria present in the silage. Experimental subjects included 10 bullocks (crosses of Limousine with Black and White Lowland (BWL) of 700 kg mean body weight. Silage was prepared from sugar beet leaves contaminated with soil. Plant material was ensiled in 6 PCV containers (barrels) of 200 dm3 in volume closed with a cover allowing the release of gaseous products. The ensiling process lasted 120 days. Samples for chemical and microbiological analyses were collected from three barrel depths (15, 30 and 45 cm) and were subsequently pooled to make a representative sample of 0.9 kg weight. The basic composition of the silage was determined in accordance with AOAC. The strain antagonistic to Shigella was identified by the molecular method: after isolating bacterial DNA, a PCR reaction was performed. The PCR analysis and the DNA sequence analysis showed that the organism which naturally occurs in sugar beet leaf silage and exhibits antagonistic properties to Shigella bacteria was Bacillus subtilis. Shigella spp., a pathogenic microorganism that is of particular concern to humans, was found in the mouth of cattle.

Słowa kluczowe

EN

antagonistic bacteria; bacteria; cattle; leaf silage; mouth; pathogen; sugar-beet; Bacillus subtilis; Shigella; antagonism; inhibition zone; silage; microorganism; bull; body weight; DNA sequence; polymerase chain reaction; pathogenic bacteria

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2011
• Tom: 67
• Numer: 02
• Opis fizyczny: p.120-124,fig.,ref.

Twórcy

autor

Galbas M.

• Institute of Biochemistry and Biotechnology, Poznan University of Life Sciences, Wolynska 35, 60-637 Poznan, Poland

autor

Dullin P.

autor

Selwet M.

Bibliografia

• 1. AOAC: Association of Official Analytical Chemists: Official Methods of Analysis, Washington, DC 1990.
• 2. DLG - Futterwerttabellen. Wiederkäuer. DLGVerl. Frankfurt am Main 1997.
• 3. Jonsson A.: Growth of Clostridium tyrobutiricum during fermentation and aerobic deterioration of grass silage. J. Sci. Food Agric. 1997, 54, 557-568.
• 4. Kraszewska J., Wzorek W., Sztando E., Raczyńska-Cabaj A.: Aktywność antagonistyczna bakterii fermentacji mlekowej z gatunku Lactobacillus plantarum. Acta Sci. Pol. Techno Aliment. 2005, 4, 39-52.
• 5. Macgregor Ch. A.: Directory of feeds and feed ingredients. Hoard's Dairyman Books. USA 2000, 80.
• 6. Mannanov R. N., Sattarova R. K.: Antibiotics produced by Bacillus bacteria. Chemistry of Natural Compounds 2001, 3 7, 117-123.
• 7. Markiewicz Z., Kwiatkowski Z. A.: Bakterie, antybiotyki i lekooporność. Wydawnictwo Naukowe PWN 2006, 250.
• 8. McDonald P., Henderson A. R., Heron S. J. E.: The Biochemistry of Silage. Marlow, Bucks, UK: Chalcombe Publications 1991, 340.
• 9. Nagao J.: Lantibiotics: Insight and Foresight for New Paradigm. J. Biosci. Bioengin. 2006, 102, 139-149.
• 10. Stypułkowska-Misiurewicz H., Gonera E.: Czerwonka bakteryjna w 2002 roku. Przegl. Epidemiol. 2004, 58, 77-84.
• 11. Tagg J. R., Mcgiven A. R.: Assay System for Bacteriocins. Appl. Microbiol. 1971, 21, 943.
• 12. Wang R. F., Cao W. W., Cerniglia C. E.: A universal protocol for PCR detection of 13 species of foodborne pathogens in foods. J. Appl. Microbiol. 1997, 83, 727-736.