Histopathology of Daucus carota L. root cells treated with toxic metabolites produced by Alternaria radicina and A. alternata

• Autorzy: Tylkowska K. , Bagniewska-Zadworna A. , Grabarkiewicz-Szczesna J. , Szopinska D. , Dorna H. , Zenkteler E.
• Języki publikacji: EN

Abstrakty

EN

Vascular storage parenchyma cells of carrot roots were treated with methanol solutions of radicinin and epiradicinol produced by Alternaria radicina and with alternariol and alternariol methyl ether produced by A. alternata at concentrations of 25 µg/ml and 250 µg/ml, as well as culture filtrates of both fungi. Cell ultrastructure was observed by TEM. No visible changes were noted after treatment with 25 µg/ml toxin solutions. The most extensive plication of cell membranes, and sometimes also cell walls, and the formation of numerous vesicles in the cytoplasm, was observed in cells treated with the higher concentration of toxins. Plasma membrane withdrawal and vésiculation, microvacuole formation, and accumulation of plastoglobuli in chromoplasts also occurred. No changes in the structure of endoplasmic reticulum and dictyosomes were noted. The responses of cell structures to particular toxins were nonspecific. Treatment with culture filtrates from A. radicina resulted in the occurrence of osmiophilic, electron-dense substance in the cytoplasm and plastoglobuli. All alterations induced by filtrates were more extensive than those resulting from toxin solutions, but membrane integrity was not disturbed after any of the treatments.

Słowa kluczowe

EN

Daucus carota; root cell; histopathology; carrot; root; toxic metabolite; Alternaria radicina; Alternaria alternata; pathogen; parenchyma cell; cell membrane; cell wall; cytoplasm; toxin; plasma membrane; alternariol; alternariol methyl ether; radicinin; ultrastructure

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2008
• Tom: 50
• Numer: 1
• Opis fizyczny: p.27-34,fig.,ref.

Twórcy

autor

Tylkowska K.

• Department of Seed Science and Technology, Poznan University of Life Sciences, Baranowo, Szamotulska 28, 62-081 Przezmierowo, Poland

autor

Bagniewska-Zadworna A.

autor

Grabarkiewicz-Szczesna J.

autor

Szopinska D.

autor

Dorna H.

autor

Zenkteler E.

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