The MAPK-dependent regulation of the Jagged/Notch gene expression by VEGF, bFGF or PPAR gamma mediated angiogenesis in HUVEC

• Autorzy: Kiec-Wilk B. , Grzybowska-Galuszka, J. , Polus A. , Pryjma J. , Knapp A. , Kristiansen K.
• Języki publikacji: EN

Abstrakty

EN

The Jagged-Notch signalling, plays a crucial role in cell differentiation. Angiogenesis, is regulated by VEGF, bFGF as well as by the free fatty acid metabolites , which are regulators of transcription factors such as peroxisome proliferation activating receptors (PPARs). The study analyzed the signalling pathways involved in the regulation of Jagged-1/Notch-4 expression in endothelial cells (HUVECs) in response to VEGF, bFGF and PPAR- exogenous activator - ciglitazone. HUVECs were incubated with investigated substances for 24 hours, with or without the presence of the MAP-kinases inhibitors were used. Jagged-1 and Notch-4 gene expression was determined using quantitative Real-Time PCR. The Jagged-1/Notch-4 protein expression was compared by flow cytometry, when the phosphorylation-dependent activation of kinases was estimated by Western-blot method. The opposite effect of VEGF, bFGF, or ciglitazone on the Jagged-1/Notch-4 expression on HUVEC was connected with the different activation of MAPKs. Ciglitazone, activated p38 MAPK pathway and simultaneously inhibited phosphorylation of p42/44 MAPK. The pro-angiogenic: bFGF and VEGF, also activated the p38 MAPK, but they did not attenuate the p42/44 MAPK phosphorylation. Maintaining of the Jagged/Notch interactions by VEGF, when down-regulation by bFGF and ciglitazone, seems to be dependent on the different effect on p38 MAPK and p42/44 MAPK pathway regulation.

Słowa kluczowe

EN

Jagged-Notch signalling; angiogenesis; cell differentiation; endothelial cell; free fatty acid; gene expression; growth factor; lipid; mitogen-activated kinase; peroxisome proliferator-activated receptor; signal transduction; vascular endothelial growth factor

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2010
• Tom: 61
• Numer: 2
• Opis fizyczny: p.217-225,fig.,ref.

Twórcy

autor

Kiec-Wilk B.

• Jagiellonian University Medical College, 15 Kopernika Street, 31-501 Krakow, Poland

autor

Grzybowska-Galuszka, J.

autor

Polus A.

autor

Pryjma J.

autor

Knapp A.

autor

Kristiansen K.

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