Combined methotrexate and coenzyme Q10 therapy in adjuvant-induced arthritis evaluated using parameters of inflammation and oxidative stress

• Autorzy: Bauerova K. , Paulovicova E. , Mihalova D. , Drafi F. , Strosova M. , Mascia C. , Biasi F. , Rovensky J. , Kucharska J. , Gvozdjakova A. , Ponist S.
• Języki publikacji: EN

Abstrakty

EN

Rheumatoid arthritis is a common severe joint disease that affects all age groups, it is thus of great importance to develop new strategies for its treatment. The aim of the present study was to examine the combined effect of coenzyme Q10 (CoQ10) and methotrexate (MTX) on the progression of adjuvant-induced arthritis in rats. Adjuvant arthritis (AA) was induced by a single intradermal injection of heat-inactivated Mycobacterium butyricum in incomplete Freund's adjuvant. The experiments included healthy animals, arthritic animals not treated, arthritic animals treated with CoQ10, with methotrexate, and with a combination of CoQ10 and methotrexate. The two latter groups received a daily oral dose of 20 mg/kg b.w. of CoQ10, either alone or with methotrexate in an oral dose of 0.3 mg/kg b.w. twice a week. We found that CoQ10 potentiated both the antiarthritic (decrease of hind paw volume) and the antioxidant effect of methotrexate on the level of oxidation of proteins (suppression of protein carbonyl level in plasma) as well as lipoperoxidation (suppression of levels of HNE-adducts and MDA-adducts to plasma proteins). The same effect was observed for plasmatic levels of CoQ9 and IL-1α, and partially also for γ-glutamyltransferase activity assessed in joints and spleen. Moreover, the combination therapy improved the functionality of peripheral blood neutrophils in AA, with a balancing effect on the immunosuppression caused by MTX monotherapy. In summary, combined administration of CoQ10 and methotrexate suppressed arthritic progression in rats more effectively than did MTX alone. This finding may help improve treatment of rheumatoid arthritis.

Słowa kluczowe

EN

combined therapy; methotrexate; coenzyme Q10; therapy; adjuvant-induced arthritis; arthritis; inflammation; oxidative stress; human disease; rheumatoid arthritis; patient

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2010
• Tom: 57
• Numer: 3
• Opis fizyczny: p.347-354,fig.,ref.

Twórcy

autor

Bauerova K.

• Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovakia

autor

Paulovicova E.

autor

Mihalova D.

autor

Drafi F.

autor

Strosova M.

autor

Mascia C.

autor

Biasi F.

autor

Rovensky J.

autor

Kucharska J.

autor

Gvozdjakova A.

autor

Ponist S.

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