Effects of peroxisome proliferator-activated receptors-gamma ligands on dextran sodium sulphate-induced colitis in rats

• Autorzy: Celinski K. , Dworzanski T. , Korolczuk A. , Piasecki R. , Slomka M. , Madro A. , Fornal R.
• Języki publikacji: EN

Abstrakty

EN

Recent studies indicate the involvement of peroxisone proliferator-activated receptor- (PPAR-) in the inflammatory reaction. The exact mechanism of PPAR- action has not been elucidated. It is supposed that PPAR- regulates transcription of genes responsible for encoding cytokines involved in the inflammatory response. The latest studies, carried out to explain the pathogenesis of non-specific colitis, confirm beneficial effects of PPAR- agonists on attenuation of colon inflammation. The aim of the present study was to assess the effects of nuclear PPAR- activity on the course of experimental acute colitis induced by intragastric administration of dextran sodium sulphate (DSS) using the PPAR- agonist rosiglitazone and the antagonist BADGE in rats. Colitis in Wistar rats was induced by 1.5% DSS administered in drinking water for 8 days. Animals with induced colitis received rosiglitazone, bisphenol A diglycidyl ether (BADGE) or both substances. After decapitation, colons were macroscopically and histopathologically evaluated. Levels of interleukin-1ß (IL-1ß), interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor- (TNF-) and myeloperoxidase (MPO) were determined in serum and colon homogenates using ELISA. In rats with experimentally induced colitis receiving rosiglitazone, the inflammatory reaction was found to be markedly limited; ulceration, oedema and infiltration activity were reduced. The activated PPAR- inhibit the expression of proinflammatory factors, such as IL-6, TNF-, and neutrophil chemotaxis, which was evidenced by MPO reduction in serum and colon homogenates mediated by rosiglitazone. The positive effects of rosiglitazone on expression of IL-10 were also demonstrated. During the short period of observation, BADGE did not increase histopathological inflammatory markers.

Słowa kluczowe

EN

bisphenol a; colitis; dextran sulphate sodium; inflammatory bowel disease; inflammatory reaction; interleukin 10; interleukin 1beta; interleukin 6; ligand; myeloperoxidase; peroxisome proliferator-activated receptor gamma; rat; tumour necrosis factor-alpha; ulcerative colitis

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2011
• Tom: 62
• Numer: 3
• Opis fizyczny: p.347-356,fig.,ref.

Twórcy

autor

Celinski K.

• Department of Gastroenterology, Medical University of Lublin, Jaczewskiego 8, 20-954 Lublin, Poland

autor

Dworzanski T.

autor

Korolczuk A.

autor

Piasecki R.

autor

Slomka M.

autor

Madro A.

autor

Fornal R.