PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2011 | 20 | 3 |
Tytuł artykułu

The effects of cadmium on common carp erythrocyte morphology

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The effect of short-term and long-term in vivo exposure to cadmium on the morphology of common carp erythrocytes was evaluated, and comparison of in vitro toxicity of various cadmium salts to these cells was also done. Juvenile carps were subjected to 3 h exposure at 6.5 mg/l or to a 4 week exposure at 0.65 mg/l of cadmium. Full blood was incubated for 2 h with cadmium chloride, sulfate, or nitrate at the concentration of 5 μl/ml of blood. All exposures resulted in a significant increase in frequency of abnormal erythrocytes. Anomalies included chromatin condensation at the nucleus border, nuclear malformation, cell body malformation, cytoplasm vacuolation, and swelling and hemolysis, with nuclear anomalies being the most frequent. In fish subjected to long-term cadmium exposure (and in some degree in fish after short-term exposure) an increase in erythroblast frequency occurred, which indicates hematological compensation for erythrocyte damage. A high percentage of abnormal erythrocytes observed under in vitro conditions (also in the control) indicates very high sensitivity of these cells to experimental factors. Cadmium nitrate induced more anomalies in carp erythrocytes (particularly nucleus malformations and cytoplasm vacuolation) than chloride and sulfate. The obtained results confirmed the genotoxic and cytotoxic properties of cadmium and showed that fish erythrocytes are good models for cytotoxicity studies.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
20
Numer
3
Opis fizyczny
p.783-788,fig.,ref.
Twórcy
autor
  • Department of Animal Physiology, University of Podlasie in Siedlce, B.Prusa 12, 08-110 Siedlce, Poland
autor
Bibliografia
  • 1. SJOBECK M. L., HAUX C., LARSSON A., LITHNER G. Biochemical and hematological studies on perch, Perca fluviatilis, from the cadmium-contaminated river Eman. Ecotoxicol. Environ. Saf. 8, 303, 1984.
  • 2. GILL T.S, PANT J.C. Erythrocytic and leukocytic responses to cadmium poisoning in a freshwater fish, Puntius conchonius ham. Environ. Res. 36, 327, 1985.
  • 3. RUPARELIA S. G., VERMA Y., SAYIED S. R., RAWALL U. M. Effect of cadmium on blood of tilapia, Oreochromis mossambicus (Peters), during prolonged exposure. Bull. Environ. Contam. Toxicol. 45, 305, 1990.
  • 4. SAXENA M. P., GOPAL K., JONES W., RAY P. K. Immune responses to Aeromonas hydrophila in cat fish (Heteropneustes fossillis) exposed to cadmium and hexachlorocyclohexane. Bull. Environ. Contam. Toxicol. 48, 194, 1992.
  • 5. JOSHI P. K., BOSE M., HARISH D. Changes in certain haematological parameters in a siluroid cat fish Clarias batrachus (Linn) exposed to cadmium chloride. Pollut. Res. 21, 129, 2002.
  • 6. KARUPPASAMY R., SUBATHRA S., PUVANESWARI S. Hematological responses to exposure to sublethal concentration of cadmium in air breathing fish, Channa punctatus. J. Environ. Biol. 26, 123, 2005.
  • 7. GILL T. S., EPPLE A. Stress-related changes in hematological profile of the American eel (Anguilla rostrata). Ecotoxicol. Environ. Saf. 25, 227, 1993.
  • 8. VOSYLIENE M. Z. The effect of heavy metals on haematological indices of fish (survey). Acta Zool. Lituanica 9, 76, 1999.
  • 9. ZENI C., BOVOLENTA M. R., STAGNI A. Occurrence of echinocytosis in circulating RBC of black bullhead, Ictalurus melas (Rafinesque), following exposure to an anionic detergent at sublethal concentrations. Aquat. Toxicol. 57, 217, 2002.
  • 10. WITESKA M. The effect of toxic chemicals on blood cell morphology in fish. Fresenius Environmental Bulletin 12a, 1, 2004.
  • 11. HOFER R., WEYRER S., KOCK G., PITTRACHER H. Heavy metal intoxication of arctic charr (Salvelinus alpinus) in a remote acid Alpine lake. FAO/EIFAC/XVII/92/Symp. E31, Lugano, Switzerland. 1992.
  • 12. WITESKA M., KONDERA E., SZYMAŃSKA M., OSTRYSZ M. Hematological changes in common carp (Cyprinus carpio L.) after a short-term lead (Pb) exposure. Polish Journal of Environmental Studies 19, 825, 2010.
  • 13. SHARMA K. P., SHARMA S., SHARMA S., SINGH P. K., KUMAR S., GROVER R., SHARMA P. K. A comparative study on characterization of textile wastewaters (untreated and treated) toxicity by chemical and biological tests. Chemosphere 69, 48, 2007.
  • 14. EVELYN T. P. T., TRAXLER G. S. Viral erythrocytic necrosis: natural occurrence in Pacific salmon and experimental transmission. J. Fish. Res. Bd. Can. 35, 903, 1978.
  • 15. EIRAS J. C., COSTA G., BISCOITO M, DAVIES A. J. Suspected viral erythrocytic necrosis (VEN) in the intertidal fish Mauligobius maderensis from Madeira, Portugal. J. mar. biol. Ass. U. K. 76, 545, 1996.
  • 16. BUCKLEY J. A. Heinz body hemolytic anemia in Coho salmon (Oncorhynchus kisutch) exposed to chlorinated wastewater. J. Fish. Res. Bd. Can. 34, 224, 1976.
  • 17. GUHA B., KHUDA-BUKHSH A. R. Efficacy of vitamin-C (L-ascorbic acid) in reducing genotoxicity in fish (Oreochromis mossambicus) induced by ethyl methane sulphonate. Chemosphere 47, 49, 2002.
  • 18. CAVAS T., GARANKO N. N., ARKHIPCHUK V. V. Induction of micronuclei and binuclei in blood, gill and liver cells of fishes chronically exposed to cadmium chloride and copper sulphate. Food and Chem. Toxicol. 43, 569, 2005.
  • 19. ERGENE S., CAVAS T., CELIK A., KOLELI N., KAYA F., KARAHAN A. Monitoring of nuclear abnormalities in peripheral erythrocytes of three fish species from the Goksu Delta (Turkey): genotoxic damage in relation to water pollution. Ecotoxicology 16, 385, 2007.
  • 20. STRUNJAK-PEROVIC I., COZ-RAKOVAC R., TOPIC POPOVIC N., JADAN M. Seasonality of nuclear abnormalities in gilthead sea bream Sparus aurata (L.) erythrocytes. Fish Physiol. Biochem. 35, 287, 2009.
  • 21. BOGE G., ROCHE H. Cytotoxicity of phenolic compounds on Dicentrarchus labrax erythrocytes. Bull. Environ. Contam. Toxicol. 57, 171, 1996.
  • 22. GILL T. S., PANT J. C. Chromatin condensation in the erythrocytes of fish following exposure to cadmium. Bull. Environ. Contam. Toxicol. 36, 199, 1986.
  • 23. GILL T. S., PANT J. C. Hematological and pathological effects of chromium toxicosis in the freshwater fish, Barbus conchonius Ham. Water, Air & Soil Pollut. 35, 241, 1987.
  • 24. GWOŹDZIŃSKI K. Structural changes of proteins in fish red blood cells after copper and mercury treatment. Arch. Environ. Contam. Toxicol. 23, 426, 1992.
  • 25. WITESKA M., JEZIERSKA B., WOLNICKI J. Respiratory and hematological response of tench Tincatinca (L.) to a short-term cadmium exposure. Aquacult. Int. 14, 141, 2006.
  • 26. HOUSTON A. H., KEEN J. E. Cadmium inhibition of erythropoiesis in goldfish, Carassius auratus. Can. J. Fish. Aquat. Sci. 41, 1829, 1984.
  • 27. CELIK A., COMELEKOGLU U., YALIN S. A study on the investigation of cadmium chloride genotoxicity in rat bone marrow using micronucleus test and chromosome aberration analysis. Toxicol. Industr. Health 21, 243, 2005.
  • 28. CELIK A., BUYUKAKILLI B., CIMEN B., TASDELEN B., OZTURK M. I., EKE D. Assessment of cadmium genotoxicity in peripheral blood and bone marrow tissues of male Wistar rats. Toxicology Mechanisms and Methods 19, 135, 2009.
  • 29. PAPOUTSOGLOU S., E., ABEL P., D. Sublethal toxicity and accumulation of cadmium in Tilapia aurea. Bull. Environ. Contam. Toxicol. 41, 404, 1988.
  • 30. ROMERO D., HERNANDEZ-GARCIA A., TAGLIATI C.A., MARTINEZ-LOPEZ E., GARCIA-FERNANDEZ A.J. Cadmium- and lead-induced apoptosis in mallard erythrocytes (Anas platyrhynchos). Ecotoxicol. Environ. Saf. 72, 37, 2009.
  • 31. MOJZIS J., NISTIAR F. Cadmium-induced changes in cation osmotic haemolysis in rats. Environ. Toxicol. Pharmacol. 8, 79, 2000.
  • 32. VERBOST P. M., FLIK G., PANG P. K. T., LOCK R. A. C., WENDELAAR BONGA S. E. Cadmium inhibition of the erythrocyte Ca2+ pump. Jour. Biol. Chem. 264, 5613, 1989.
  • 33. SUWALSKY M., VILLENA F., NORRIS B., CUEVAS, SOTOMAYOR C. P. Cadmium-induced changes in the membrane of human erythrocytes and molecular models. Jour. Inorg. Biochem. 98, 1061, 2004.
  • 34. LIONETTO M. G., CARICATO R., ERROI E., GIORDANO M. E., SCHETTINO T. Potential application of carbonic anhydrase activity in bioassay and biomarker studies. Chemistry and Ecology, 22, 119, 2006.
  • 35. BEKTAS S., HISAR O., HISAR S. A., YANIK T. Inhibition effect of cadmium on carbonic anhydrase in rainbow trout (Oncorhynchus mykiss). Fresenius Environmental Bulletin, 17, 795, 2008.
  • 36. GAROFANO J. S., HIRSHFIELD H. I. Peripheral effects of cadmium on the blood and head kidney in the brown bullhead (Ictalurus nebulosus). Bull. Environ. Contam. Toxicol. 28, 552, 1982.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.dl-catalog-3cbf8ff5-43a4-4926-ab00-6b477f71ed6c
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.