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2011 | 55 | 1 |

Tytuł artykułu

Isolation, biotyping, and serotyping of Yersinia enterocolitica strains from fattening pigs

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The study has been taken up to collect more data on Yersinia enterocolitica isolated from pigs as the main reservoir and source of infection with strains pathogenic for humans. Bacteriological examinations, bio- and serotyping were conducted on 616 rectal swabs, taken from 308 fattening pigs. Two samples were taken from each animal to determine the ability of Y. enterocolitica to grow under different temperature conditions (warm and cold culture). It has been proven that low temperature constitutes a suitable culture condition. 138 Yersinia enterocolitica strains were isolated (22.40%), most of which (65.22%) were obtained in cold culture and 99.28% included in biotype 3 (one strain belonged to biotype 2). Serotyping yielded a positive result in 107 strains with the diagnostic serum for antigen O:3, in 18 - with the serum for antigen O:9, and 13 strains were determined to be non-typable. The results indicated that asymptomatic infections with Y. enterocolitica strains of the biotypes and serotypes pathogenic for humans are common in pig population.

Wydawca

-

Rocznik

Tom

55

Numer

1

Opis fizyczny

p.39-43,ref.

Twórcy

  • Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland
autor

Bibliografia

  • 1. Farzan A., Friendship R.M., Cook A., Pollari F.: Occurrence of Salmonella. Campylobacter, Yersinia enterocolitica, Escherichia coli O157 and Listeria monocytogenes in swine. Zoonoses Public Health 2009, Jal 23, (Epub ahead of print).
  • 2. Fredriksson-Ahomaa M., Stolle A., Korkeala H.: Molecular epidemiology of Yersinia enterocolitica infections. FEMS Immunol Med Microbiol 2006, 47, 315-329.
  • 3. Fredriksson-Ahomaa M., Stolle A., Stephan R.: Prevalence of pathogenic Yersinia enterocolitica in pigs slaughtered at a Swiss abattoir. Int J Food Microbiol 2007, 119, 207-212.
  • 4. Fukushima H., Maruyama K., Omori I., Ito K., Kaneko S.: Isolation of sucrose-negative Yersinia enterocolitica biotype 3 serotype 03 strains and their pathogenicity. Curr Microbiol 1998, 4, 199-202.
  • 5. Gürtler M., Alter T., Kasimir S., Linnebur M., Fehlhaber K.: Prevalence of Yersinia enterocolitica in fattening pigs. J Food Prot 2005, 68, 850-854.
  • 6. Kaneko S., Maruyama T.: Pathogenicity of Yersinia enterocolitica serotype O3 biotype 3 strains. J Clin Microbiol 1987, 25, 454-455.
  • 7. Kelesidis T., Balba G., Worthington M.: Axillary abscess in a patient with Yersinia enterocolitica infection as a result of exposure to pork. Am J Med 2008, 121, 1.
  • 8. Kot B., Trafny E.A., Jakubczak A.: Application of multiplex PCR for monitoring colonization of pig tonsils by Yersinia enterocolitica, including biotype 1A, and Yersinia pseudotuberculosis. J Food Prot 2007, 70, 1110- 1115.
  • 9. Kuehni-Boghenbor K., On S.L.W., Kokotovic B., Baumgartner A., Wassenaar T.M., Wittwer M., Bissig- Choisat B., Frey J.: Genotyping of human and porcine Yersinia enterocolitica. Yersinia intermedia, and Yersinia bercovieri strains from Switzerland by amplified fragment length polymorphism analysis. Appl Environ Microbiol 2006, 6, 4061-4066.
  • 10. Laukkanen R., Martinez P.O., Siekkinen K.M., Ranta J., Maijala R., Korkeala H.: Contamination of carcasses with human pathogenic Yersinia enterocolitica 4/O:3 originates from pigs infected on farms. Foodborne Pathog Dis 2009, 6, 681-688.
  • 11. Laukkanen R., Hakkinen M., Lundén J., Fredriksson- Ahomaa M., Johansson T., Korkeala H.: Evaluation of isolation methods for pathogenic Yersinia enterocolitica from pig intestinal content. J Appl Microbiol 2010, 108, 956-964.
  • 12. Milnes A.S., Stewart I., Clifton-Hadley F.A., Davies R.H., Newell D.G., Sayers A.R., Cheasty T., Cassar C., Ridley A., Cook A.J., Evans S.J., Teale C.J., Smith R.P., McNally A., Toszeghy M., Futter R., Kay A., Paiba G.A.: Intestinal carriage of verocytotoxigenic Escherichia coli O157, Salmonella, thermophilic Campylobacter and Yersinia enterocolitica, in cattle, sheep and pigs at slaughter in Great Britain during 2003. Epidemiol Infect 2008, 136, 739-751.
  • 13. Nesbakken T., Iversen T., Eckner K., Lium B.: Testing of pathogenic Yersinia enterocolitica in pig herds based on the natural dynamic of infection. Int J Food Microbiol 2006, 111, 99-104.
  • 14. Nesbakken T., Iversen T., Lium B.: Pig herds free from human pathogenic Yersinia enterocolitica. Emerg Infect Dis 2007, 13, 1860-1864.
  • 15. Platt-Samoraj A.: Pigs and other animal species as a reservoir and source of Yersinia enterocolitica infection. Doctoral thesis, Faculty of Veterinary Medicine, UW-M Olsztyn, Poland, 1995.
  • 16. Polski Komitet Normalizacyjny: Polska Norma PN-EN ISO 10273 Mikrobiologia żywności i pasz. Horyzontalna metoda wykrywania przypuszczalnie chorobotwórczych Yersinia enterocolitica, 2004.
  • 17. Sihvonen L.M., Haukka K., Kuusi M., Virtanen M.J., Siitonen A.: Yersinia enterocolitica and Y. enterocolitica- like species in clinical stool specimens of humans: identification and prevalence of bio/serotypes in Finland. Eur J Clin Microbiol Infect Dis 2009, 28, 757-765.
  • 18. Singh I., Bhatnagar S., Virdi J.S.: Isolation and characterization of Yersinia enterocolitica from diarrhoeic human subjects and other sources. Curr Sci 2003, 84. 1353-1355.
  • 19. Wehebrink T., Kemper N., Grosse Beilage E., Krieter J.: Prevalence of Campylobacter spp. and Yersinia spp. in the pig production. Berl Munch Tierarzil Wochenschr 2008, 121, 27-32.
  • 20. Wesley I.V., Bhaduri S., Bush E.: Prevalence of Yersinia enterocolitica in market weight hogs in the United States. J Food Prot 2008, 71, 1162-1168.
  • 21. www.efsa.europa.eu/fr/scdocs/doc/1496.pdf The European Food Safety Authority summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in the European Union in 2008.
  • 22. Zaremba M., Grala-Kałużna A.: Epizootiology of yersiniosis. Medycyna Wet 1981, 37, 281-284.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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