Identyfikacja grzybów rodzaju Fusarium techniką SCAR-PCR

• Autorzy: Baturo A. , Lukanowski A. , Lenc L. , Sadowski C.

Warianty tytułu

EN

Identification of fungi of the genus Fusarium using SCAR-PCR technique...........................

• Języki publikacji: PL

Abstrakty

EN

The identification concerned 71 isolates obtained from potato tubers which were identified on the basis of morphological features as Fusarium sambucinum, and 46 isolates from wheat, barley rye, triticale and potato. Which were identified as F. culmorum. Molecular analyses using SCARPCR revealed incorrect identification of 5 isolates obtained from potato: two were earlier identified as F. sambucinum and 3 as F. culmorum. This indicates the fact that F. culmorum and F. sambucinum occurring on potato can be mistaken while using a traditional microscopic identification. The research confirmed the usefulness of species-specific primers in diagnostics of plant pathogens. It was stated that for obtaining fully reliable results, it is advisable to use, besides a microscopic method, a molecular analysis.

Słowa kluczowe

PL

badania mikroskopowe; diagnostyka molekularna; Fusarium culmorum; Fusarium sambucinum; grzyby chorobotworcze; identyfikacja; lancuchowa reakcja polimerazy; markery SCAR

EN

microscopy research; molecular diagnostics; Fusarium culmorum; Fusarium sambucinum; pathogenic fungi; identification; fungi; polymerase chain reaction; SCAR marker

• Wydawca: -
• Czasopismo: Progress in Plant Protection
• Rocznik: 2010
• Tom: 50
• Numer: 1
• Opis fizyczny: s.15-18,fot.,bibliogr.

Twórcy

autor

Baturo A.

• Katedra Fitopatologii i Mikologii Molekularnej, Uniwersytet Technologiczno-Przyrodniczy im. Jana i Jędrzeja Śniadeckich w Bydgoszczy, ul.Koreckiego 20, 85-225 Bydgoszcz

autor

Lukanowski A.

autor

Lenc L.

autor

Sadowski C.

Bibliografia

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