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2010 | 62 | 4 |
Tytuł artykułu

Fenotypowe cechy pałeczek Pseudomonas putida izolowanych z różnych środowisk

Autorzy
Warianty tytułu
EN
Phenotypic characteristics of Pseudomonas putida isolated from different environments
Języki publikacji
PL
Abstrakty
PL
Badaniami objęto 34 szczepy P. putida, z których 27 izolowano z gleby zanieczyszczonej pochodnymi ropy naftowej, 3 z biopreparatów stosowanych w procesach biodegradacji zanieczyszczeń oraz 4 z ran i owrzodzeń. Podjęto próbę wewnątrzgatunkowego różnicowania szczepów P. putida wykorzystując ich aktywność biochemiczną, wzory lekowrażliwości i określając zymotypy. Stwierdzono przydatność tych metod w diagnostyce mikrobiologicznej pałeczek P. putida.
EN
Various typing methods have been suggested to differentiate isolates of P. putida species with the aim of developing epidemiological tools. 34 P. putida strains were isolated from the samples of crude oil and oil derivates contaminated soil (n = 27), biopreparates used for biodegradation of soil contamination (n = 3) and hospital materials ( n = 4). The biochemical typing was assessed using ID32GN tests (bio-Merieux). The strains were grouped into 11 biotypes. Antibiotic-containing discs were used for routine antibiograms by disc diffusion assay. The strains were most resistant to cefoperazone and ticarcillin (29,4% and 26,5% of strains, respectively). The intracellular esterases of P. putida were separated by Polyacrylamid gel electrophoresis and stained with Fast Blue using alpha-naphthyl acetate, beta-naphthyl propionate and indoxyl acetate as substrates. On the basis of the indicators dye migration the electrophoresis process was observed. By calculating the RF (retention factor) the distribution of stripes was obtained with great accuracy. The use of biochemical tests, sensitivity tests and zymotyping provides the possibility to inter- and intraspecies differentiation of P. putida.
Wydawca
-
Rocznik
Tom
62
Numer
4
Opis fizyczny
s.361-368,tab.,fot.,bibliogr.
Twórcy
autor
  • Wielkopolskie Centrum Onkologii im.Marii Skłodowskiej Curie
Bibliografia
  • 1. Abbott Y, Leonard FC, Markey BK. Detection of three distinct genetic lineages in methicillin- resistant Staphylococcus aureus (MRSA) isolates from animals and veterinary personnel. Epidemiol Infect 2010; 138: 764-71.
  • 2. Bogiel T, Mikucka A, Deptuła A, Gospodarek E. Wykorzystanie metod fenotypowych do wewnątrzgatunkowego różnicowania meticylinoopornych szczepów Staphylococcus epidermidis - analiza porównawcza. Med Dośw Mikrobiol 2009; 61:11 -20.
  • 3. Branger C, Goullet P, Boutonnier A, Fournier JM. Correlation between esterase electrophoretic types and capsular polysaccharide types 5 and 8 among methicillin-susceptible and methicillin- resistant strains of Staphylococcus aureus. J Clin Microbiol 1990; 28: 150-1.
  • 4. Carpenter RJ, Hartzell JD, Forsberg JA i inni. Pseudomonas putida war wound infection in a US Marine: A case report and review of the literature. J Infect 2008; 56: 234-40.
  • 5. Cybulski Z, Dziurla E, Kaczorek E, Olszanowski A. The influence of emulsifiers on hydrocarbon biodégradation by Pseudomonadaceae and Bacillaceae strains. Spill Sci Technol Bull 2003; 8: 503-7.
  • 6. Cybulski Z, Kjaeldgaard P. Resistance to oral antibiotics in urinary coliform strains isolated between 2000 and 2008 in Sonderborg area, Denmark. Int J Antimicrob Agents 2010; 35: 516-7.
  • 7. Cybulski Z, Krzemińska-Jaśkowiak E, Majewski P i inni. Enzyme production and biotypes of vaginal Candida albicans. Pol J Microbiol 2005; 54: 227-31.
  • 8. Jiang Y, Ye J, Wu H, Zhang H. Cloning and expression of the polyhydro- xyalkanote depolymerase gene from Pseudomonas putida, and characterization of the gene product. Biotechnol Lett 2004; 26: 1585-8.
  • 9. Jung HC, Kwon SJ, Pan JG. Display of a thermostable lipase on the surface of a solvent-resistant bacterium, Pseudomonas putida GM730, and its applications in whole-cell biocatalysis. BMC Biotechnol 2006; 6: 23.
  • 10. Kaczorek E, Olszanowski A, Cybulski Z. Analysis of surface tension during biodegradation of hydrocarbons. Pol J Environ Stud 2005; 2: 185-9.
  • 11. Lee YL, Thrupp L. Genotyping by restriction endonuclease analysis compared to phenotyping by antibiogram for typing methicillin-resistant Staphylococcus aureus strains colonizing patients in a nursing home. Infect Control Hosp Epidemiol 2000; 21:218-21.
  • 12. Lei Y, Mulchandani A, Chen W. Improved degradation of organophosphorus nerve agents and p-nitrophenol by Pseudomonas putida JS444 with surface-expressed organophosphorus hydrolase. Biotechnol Prog 2005; 21: 678-81.
  • 13. Międzobrodzki J, Malachowa N, Markiewski T i inni. Różnicowanie izolatów Staphylococcus aureus w oparciu o cechy fenotypowe. Postępy Hig Med Dośw 2008; 62: 322-7.
  • 14. National Committee for Clinical Laboratory Standards. Performance Standards for Antimicrobial Susceptibility: Eleventh Informational Supplement M100-S11. NCCLS Wayne PA USA 2001.
  • 15. Nishi J, Yoshinaga M, Miyanohara H i inni. An epidemiologic survey of methicillin-resistant Staphylococcus aureus by combined use of mec-HVR genotyping and toxin genotyping in a university hospital in Japan. Infect Control Hosp Epidemiol 2002; 23: 506-10.
  • 16. Rolston KV, Kontoyiannis DP, Yadegarynia D, Raad II. Nonfermentative gram-negative bacilli in cancer patients: increasing frequency of infection and antimicrobial susceptibility of clinical isolates to fluoroquinolones. Diagn Microbiol Infect Dis 2005; 51: 215-8.
  • 17. Rossney AS, Lawrence MJ, Morgan PM i inni. Epidemiological typing of MRSA isolates from blood cultures taken in Irish hospitals participating in the European Antimicrobial Resistance Surveillance System (1999-2003). Eur J Clin Microbiol Infect Dis 2006; 25: 79-89.
  • 18. Sandoval A, Arias-Barrau E, Bermejo F. Production of 3-hydroxy-n-phenylalkanoic acids by a genetically engineered strain of Pseudomonas putida. Appl Microbiol Biotechnol 2005; 67: 97-105.
  • 19. Silva CM, de Castro VL, de Oliveira PR, Maia Ade H. Influence of Pseudomonas putida AF7 inoculation on soil enzymes. Ecotoxicology 2009; 18: 1182-7.
  • 20. Zhanel GG, DeCorby M, Laing N. Antimicrobial-resistant pathogens in intensive care units in Canada: results of the Canadian National Intensive Care Unit (CAN-ICU) study, 2005-2006. Antimicrob Agents Chemother 2008;52: 1430-7.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.dl-catalog-31c80eb3-3055-4a4d-bf89-58a1f8294e5c
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