Usefulness of PCR/RFLP and ERIC PCR techniques for epidemiological study of Haemophilus parasuis infections in pigs

• Autorzy: Jablonski A. , Zebek S. , Kolacz R. , Pejsak Z.
• Języki publikacji: EN

Abstrakty

EN

Haemophilus parasuis belongs to opportunistic microorganisms of undefined virulence. The purpose of the studies was to compare suitability of PCR/RFLP in our modification and ERIC PCR for epidemiological study of domestic strains of H. parasuis. The results were evaluated taking into account two different aspects: suitability of the tests for isolating the highest possible number of clone groups and subjective evaluation of the method judged with respect to the following criteria: difficulty, availability of equipment and reagents as well as time and cost of the study. The results obtained in the present study show that the two methods used for typing of H. parasuis had high discriminatory power. Taking into account this parameter it can be concluded that ERIC PCR is more suitable than PCR/RFLP. This justifies the use of ERIC PCR for routine epidemiological analyses of mentioned pathogen. Taking into account the complexity of method used, ERIC-PCR based on random amplification of DNA, proved to be comparable to PCR/RFLP. The last mentioned technique is relatively less expensive and labour-consuming, especially when diagnostic PCR method is used for the epidemiological studies.

Słowa kluczowe

EN

animal disease; pig; animal infection; epidemiology; Haemophilus parasuis; genotyping; polymerase chain reaction; virulence; diagnosis; microorganism; DNA fragment; electrophoretic separation; environmental factor; molecular method

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2011
• Tom: 14
• Numer: 1
• Opis fizyczny: p.111-116,fig.,ref.

Twórcy

autor

Jablonski A.

• Swine Diseases Department, National Veterinary Research Institute, 57 Partyzantow Str., 24-100 Pulawy, Poland

autor

Zebek S.

autor

Kolacz R.

autor

Pejsak Z.

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