In vitro IL-4, IL-12, and IFN-gamma production by splenocytes from Ancylostoma ceylanicum infected hamsters

• Autorzy: Dlugosz E. , Wisniewski M. , Wojcik L. , Wedrychowicz H.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was the production of chicken polyclonal antibodies recognising hamster IL-4, IL-12p35, and IFN-γ, which were further used to estimate cytokine concentrations in splenocyte culture supernatants, obtained from three age groups of Ancylostoma ceylanicum infected hamsters. Worm burdens differed significantly among age groups; the younger animals were the more heavily infected. Th1 and Th2 type cytokines were detected only in cultures containing spleen cells from the oldest hamsters, which were the most resistant to hookworm infection. In cultures of cells from young more susceptible hamsters, the presence of IL-4 and IL-12 but not IFN-γ were observed.

Słowa kluczowe

EN

Ancylostoma ceylanicum; animal disease; hamster; in vitro; infected animal; interleukin 12; interleukin 4; splenocyte; cytokine concentration; nematode; dog; cat; infection; immune response

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 3
• Opis fizyczny: p.321-325,fig.,ref

Twórcy

autor

Dlugosz E.

• Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, 02-787 Warsaw, Poland

autor

Wisniewski M.

autor

Wojcik L.

autor

Wedrychowicz H.

Bibliografia

• 1. Alkazmi L., Behnke J.M.: The mucosal response to secondary infection with Ancylostoma ceylanicum in hamsters immunized by abbreviated primary infection. Parasite Immunol 2010, 32, 47-56.
• 2. Baggio V., Ott F., Fischer R.W., Gram H., Peele J., Spreng D., Schmokel H., Jungi T.W.: Production of antibodies to canine IL-lß and canine TNF to assess the role of proinflammatory cytokines. Vet Immunol Immunopathol 2005, 107, 27-39.
• 3. Behnke J.M., Guest J., Rose R.: Expression of acquired immunity to the hookworm Ancylostoma ceylanicum in hamsters. Parasite Immunol 1997, 19, 309-318.
• 4. Bethony J., Brooker S., Albonico M., Geiger S.M., Loukas A., Diemert D., Hotez P.J.: Soil-transmitted helminth infections: ascariasis, trichuriasis and hookworm. Lancet 2006, 367, 1521-1532.
• 5. Bungiro R.D., Sun T., Harrison L.M., Shoemaker C.B., Cappello M.: Mucosal antibody responses in experimental hookworm infection. Parasite Immunol 2008, 30, 293-303.
• 6. Cooper E.S., Whyte-Alleng C.A., Finzi-Smith J.S., MacDonald T.T.: Intestinal nematode infections in children: the pathophysiological price paid. Parasitology 1992, 104 (suppl.), S91-S103.
• 7. Correa-Oliveira R., Caldas I.R., Gazzinelli G.: Natural versus drug-induced resistance in Schistosoma mansoni infection. Parasitol Today 2000, 16, 397-399.
• 8. de Vries E., de Groot R., de Bruin-Versteeg S., Comans- Bitter W.M., van Dongen J.J.M.: Analysing the developing lymphocyte system of neonates and infants. Eur J Pediatr 1999, 158, 611 -617.
• 9. Dlugosz E.: Hookworm infections in human and laboratory animals - differences and similarities in immune responses. Wiad Parazytol 2005, 51, 219-225.
• 10. Fleming A.F.: Iron deficiency in the tropics. Clin Haematol 1982, 11, 365-288.
• 11. Fujiwara R.T., Geiger S.M., Bethony J., Mendez S.: Comparative immunology of human and animal models of hookworm infection. Parasite Immunol 2006, 28, 285- 293.
• 12. Fujiwara R.T., Zhan B., Mendez S., Loukas A., Bueno L.L., Wang Y., Plieskatt J., Oksov Y., Lustigman S., Bottazzi M.E., Hotez P., Bethony J.M.: Reduction of worm fecundity and canine host blood loss mediates protection against hookworm infection elicited by vaccination with recombinant Ac-16. Clin Vaccine Immunol 2007, 14, 281-287.
• 13. Garside P., Behnke J.M., Rose R.A.: Acquired immunity to Ancylostoma ceylanicum in hamsters. Parasite Immunol. 1990, 12, 247-258.
• 14. Garside P., Behnke J.M.: Ancylostoma ceylanicum: observations on host-parasite relationship during primary hookworm infection. Parasitology 1989, 98, 283-289.
• 15. Geiger S.M., Caldas I.R., Mc Glone B.E., Campi- Azevedo A.C., De Oliveira M., Brooker S., Diemert D., Correa-Oliveira R., Bethony J.M.: Stage-specific immune responses in human Necator americanus infection. Parasite Immunol 2007, 29, 347-358.
• 16. Geiger S.M., Massara C.L., Bethony J., Soboslay P.T., Carvalho O.S., Correa-Oliveira R.: Cellular responses and cytokine profiles in Ascaris lumbricoides and Trichuris trichiura infected patients. Parasite Immunol 2002, 24, 499-509.
• 17. Geiger S.M., Massara C.L., Bethony J., Soboslay P.T., Correa-Oliveira R.: Cellular responses and cytokine production in post-treatment hookworm patients from an endemic area in Brazil. Clin Exp Immunol 2004, 36, 334-340.
• 18. Ghosh K., Wu W., Antoine A.D., Bottazzi M.E., Valenzuela J.G., Hotez P.J., Mendez S.: The impact of concurrent and treated Ancylostoma ceylanicum hookworm infections on the immunogenicity of a recombinant hookworm vaccine in hamsters. J Infect Dis 2006, 193, 155-162.
• 19. Graham E.M., Jarrett O., Flynn J.N.: Development of antibodies to feline IFN-γ as tools to elucidate the cellular immune responses to FeLV. J Immunol Methods 2003, 279, 69-78.
• 20. Labiano-Abello N., Canese J., Velazquez M.E., Hawdon J.M., Wilson M.L., Hotez P.J.: Epidemiology of hookworm infection in Itagua, Paraguay: a cross sectional study. Mem Inst Oswaldo Cruz 1999, 94, 583-586.
• 21. Lewis D.B., Yu C.C., Meyer J., English B.K., Kahn S.J., Wilson C.B.: Cellular and molecular mechanisms for reduced interleukin 4 and interferon-γ production by neonatal T cells. J Clin Invest 1991, 87, 194-202.
• 22. Loukas A., Bethony J.M., Mendez S., Fujiwara R.T., Goud G.N., Ranjit N., Zhan B., Jones K., Bottazzi M.E., Hotez P.J.: Vaccination with recombinant aspartic hemoglobinase reduces parasite load and blood loss after hookworm infection in dogs. PLOS Medicine 2005, 2, 1008-1017.
• 23. Loukas A., Prociv P.: Immune responses in hookworm infections. Clin Microbiol Rev 2001, 14, 689-703.
• 24. Mendez S., Valenzuela J.G., Wu W., Hotez P.J.: Host cytokine production, lymphoproliferation, and antibody responses during the course of Ancylostoma ceylanicum infection in the Golden Syrian hamster. Infect Immun 2005, 73, 3402-3407.
• 25. Miller T.A.: Influence of age and sex on susceptibility of dogs to primary infection with Ancylostoma caninum. J Parasitol 1965,51,701-704.
• 26. Morar D., Tijhaar E., Negrea A., Hendriks J., van Haarlem D., Godfroid J., Michel A.L., Rutten V.P.M.G.: Cloning, sequencing and expression of white rhinoceros (Ceratotherium simum) interferon-γ (IFN-γ) and the production of rhinoceros IFN-γ specific antibodies. Vet Immunol Immunopathol 2007, 115, 146-154.
• 27. Quinnell R.J., Pritchard D.I., Raiko A., Brown A.P., Shaw M.A.: Immune responses in human necatoriasis: association between interleukin-5 responses and resistance to reinfection. J Infect Dis 2004, 190, 430-438.
• 28. Turaga P.S.D., Tierney T.J., Bennett K.E., McCarthy M.C., Simonek S.C., Enyong P.A., Moukatte D.W., Lustigman S.: Immunity to onchocerciasis: cells from putatively immune individuals produce enhanced levels of interleukin-5, γ-interferon, and granulocyte- macrophage colony-stimulating factor in response to Onchocerca volvulus larval and male worm antigens. Infect Immun 2000, 68, 1905-1911.
• 29. Wędrychowicz H., Orzeł A., Behnke J.M.: Host antibody recognition of surface and somatic antigens of the parasitic developmental stages of Ancylostoma ceylanicum. Acta Parasitol 1996, 41, 43-49.