EN
In Poland, it has been found so far about 260 species of plant and fungi parasitic nematodes. Among the nematodes feeding on plants there are species that have the status of quarantine pests. Before taking the decision to control nematodes, it is necessary to identify the pest species. The identification of nematodes is difficult because of the paucity of easily scorable diagnostic morphological characters. Consequently, molecular identification tools could solve this problem. For molecular identification of species are suitable different PCR-based methods, DNA barcoding and DNA microarrays. The mitochondrial cytochrome oxidase c subunit 1 (COI) gene is one of the most popular markers for species identification across the animal kingdom. Species identification based on COI sequence received name DNA barcoding. DNA microarrays, first created 20 years ago, are well established and able to differentiate hundreds of specimens simultaneously. Microarray technologies, which involve hybridization of short specific probes to target DNA and subsequent detection of the hybridization signal, have been widely used as diagnostic tools.