Screening of Actinomycetes from mangrove ecosystem for L-asparaginase activity and optimization by response surface methodology

• Autorzy: Usha R. , Mala K. K. , Venil C. K. , Palaniswamy M.
• Języki publikacji: EN

Abstrakty

EN

Marine actinomycetes were isolated from sediment samples collected from Pitchavaram mangrove ecosystem situated along the southeast coast of India. Maximum actinomycete population was noted in rhizosphere region. About 38% of the isolates produced L-asparaginase. One potential strain KUA106 produced higher level of enzyme using tryptone glucose yeast extract medium. Based on the studied phenotypic characteristics, strain KUA106 was identified as Streptomyces parvulus KUA106. The optimization method that combines the Plackett-Burman design, a factorial design and the response surface method, which were used to optimize the medium for the production of L-asparaginase by Streptomycetes parvulus. Four medium factors were screened from eleven medium factors by Plackett-Burman design experiments and subsequent optimization process to find out the optimum values of the selected parameters using central composite design was performed. Asparagine, tryptone, d))extrose and NaCl components were found to be the best medium for the L-asparaginase production. The combined optimization method described here is the effective method for screening medium factors as well as determining their optimum level for the production of L-asparaginase by Streptomycetes parvulus KUAP106.

Słowa kluczowe

EN

mangrove ecosystem; L-asparaginase; response surface methodology; actinomycete; Streptomyces parvulus; bacterial flora; marine actinomycete; sediment sample; Pitchavaram mangrove ecosystem; India; determination

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2011
• Tom: 60
• Numer: 3
• Opis fizyczny: p.213-221,fig.,ref.

Twórcy

autor

Usha R.

• Department of Microbiology, School of Life Sciences, Karpagam University, Tamil Nadu, India

autor

Mala K. K.

autor

Venil C. K.

autor

Palaniswamy M.

Bibliografia

• Abdel-Fattah Y.R., H.M. Saeed, Y. Gohar and E.L. Baz-ma. 2005. Improved production of Pseudomonas aeruginosa uricase by optimization of process parameters through statistical experimental designs. Process Biochem. 40: 1707–1714.
• Aguilar G., J. Morlon-Guyot, B. Trejo-Aguilar and J.P. Guyot. 2000. Purification and characterization of an extra cellular alpha amylase produced by Lactobacillus manihotivorans LMG 1801 (T), an amylolytic lactic acid bacterium. Enzyme Microb. Technol. 27: 406–413.
• Box G. and J.S. Hunter. 1957. Multi-factor experimental designs for exploring response surfaces. Ann. Math. Stat. 28: 195–241.
• Box G. and K.B.Wilson. 1951. On the experimetal designs for exploring response surfaces. Ann. Math. Stat. 13: 1–45.
• Cowan S.T. 1974. Cowan and Steel Manual for the Identification of Medical Bacteria 2 . Edition Cambridge, Univ. Press. Dhevandran K. and K. Annie. 1999. Antibiotic and L-asparaginase of Streptomycetes isolated from fish, shellfish, and sediments of veli estuarine along Kerala coast. Indian J. Mar. Sci. 28: 335–337.
• Gordon R.E. 1966. Some criteria for the recognition of Nocardia madura (Vincent) Blanchord. J. Gen. Microbiol. 45: 355–364.
• Gulati R., R.K. Saxena and R. Gupta. 1973. A rapid screening for L-asparaginase and glutaminase activities of microorganisms. J. Gen. Microbiol. 76: 85–99.
• Jones K. 1949. Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristics. J. Bacteriol. 57: 141–145.
• Kim H.O, J.M. Lim, J.H. Joo, S.W. Kim, H.J. Hwang, J.W. Choi and J.W. Yun. 2005. Optimization of submerged culture condition for the production of mycelial biomass and exopolysaccharides by Agrocybe cylindracea. Bioresour. Technol. 96: 1175–1182.
• Khan A.S and M.S. Ali. 2007. Mangroves- an ecosystem in peril. J. Curr. Sci. 10: 419–420.
• Kawato M and R. Shinobu. 1959. A simple technique for the microscopical observation. Mem. Osaka Unit. Lib. Arts. Educ. B Nat. Sci. 8: 114–119.
• Lechevalier M.P. and H.A. Lechevalier. 1968. Chemical composition as a criterion in the classification of aerobic actinomycetes. J. Sys. Bacteriol. 20: 435–443.
• Le Mense E.H., J. Corman, J.M. Van Lanen and A.F. Langlykke. 1947. Production of mold amylases in submerged culture. Biochem. 54: 149–159.
• Lee K.M. and D.F. Gilmore. 2005. Formulation and process modeling of biopolymer (polyhydroxyalkanoates: PHAs) production from industrial wastes by novel crossed experimental design. Process Biochem 40: 229–246.
• Locci R. 1989. Streptomyces and related genera. Bergey’s manual of systematic bacteriology. William and Wilkins company. Baltimore 4: 2451–2508.
• Majumdar A. and A. Goyal. 2008. Enhanced production of exocellular glucansucrase from Leuconostoc dextranicum NRRL B-1145 using response surface method. Bioresour. Technol. 99, 3685–3691.
• McTigue M.A., C.T. Kelly, W.M. Fogarty and E.M. Doyle. 1994. Production studies on the alkaline amylases of three alkalophilic Bacillus sp. Biotechnology Lett. 16: 569–574.
• Nawani N.N. and B.P. Kapadnis. 2005. Optimization of chitinase production using statistics based experimental designs. Process Biochem. 40: 651–660.
• Paul J.H. 1982. Isolation and characterization of a chlamydomonas L-asparaginase. Biochem. J. 203: 109–115.
• Plackett R.L. and J.P. Burman. 1946. The design of optimum multifactorial experiments. Biometrika 33: 305–325.
• Pridham T.G., P. Anderson, C. Foley, L.A. Lindenfelser, C.W. Hesselting and R.G. Benedict. 1957. A section of media for maintenance and taxonomic study of Streptomycetes. Antibiotics Ann., pp. 947–953.
• Pridham T.G. and D. Gottlieb. 1948. The utilization of carbon compounds by some actinomycetes as an aid for species determination. J. Bacteriol. 56: 107–114.
• Sahoo K. and N.K. Dhal. 2009. Potential microbial diversity in mangrove ecosystems. A review. Indian J. Marine Sci. 38: 249–256.
• Santamaria R.I., G. Del Rio, G. Saab, M.E. Rodriguez, X. Soberon and A. Lopez Marguia. 1999. Alcoholysis reactions from starch with alpha-amylases. FEBS Lett. 452: 346–350.
• Savitri A.N. and W. Azmi. 2003. Microbial L asparaginase: A potent antitumor enzyme. Indian. J. Biotechnol. 2: 184–194.
• Senthilkumar S.R., B. Ashokkumar, K. Raj Chandra and P. Gunasekaran. 2005. Optimization of medium composition for alkali-stable xylanase production by Aspergillus fischeri Fxn 1 in solid state fermentation using central composite rotary design. Bioresour Technol. 96: 1380–1386.
• Shirling E.B. and D. Gottlieb. 1966. Methods for characterization of Streptomyces species. Int. J. Syst. Bacteriol. 16: 313–340.
• Venil C.K., K. Nanthakumar, K. Karthikeyan and P. Lakshmanaperumalsamy. 2009. Production of L-asparaginase by Serratia marcescens SB08: Optimization by response surface methodology. Iranian J. Biotechnol. 7: 10–18.
• Wang Y.X. and Z.X. Lu. 2005. Optimization of processing parameters for the mycelial growth and extracellular polysaccharide production by Boletus spp. ACCC 50328. Process Biochem. 40: 1043–1051.
• Wriston J.C and T.O. Yellin. 1973. L-asparaginase: a review. Adv. Enzymol. 39: 185–248.