Effect of high dietary copper on the expression of hypothalamic appetite regulators in weanling pigs

• Autorzy: Zhu D. , Yu B. , Ju C. , Mei S. , Chen D.
• Języki publikacji: EN

Abstrakty

EN

The current study evaluated appetite-related gene expression of neuropeptide Y (NPY), orexin, type 2 orexin receptor (OrexinR2), proopiomelanocortin (POMC), melanocortin-4 receptor (MC4R), AMP-activated protein kinase α2 subunit (AMPKα2) and long-form leptin receptor (LeptinRb) in hypothalamus in response to copper (Cu) supplementation. One hundred crossbred pigs were assigned to four groups of 25 pigs, each comprising five replicates of 5 animals. Groups were then randomly assigned to treatments consisted of 1. control (10 mg/kg CuSO4), 2. 100 mg/kg CuSO4, 3. 175 mg/kg CuSO4, 4. 250 mg/kg CuSO4. On d 21 of the experiment 5 pigs from each group were slaughtered and the hypothalami were collected for determination of appetite-regulating genes mRNA expression level. The results showed that average daily feed intake and average daily gain were higher (P<0.05) in 250 and 175, 250 mg/kg Cu supplemented groups, respectively, than in the 10 mg/kg group. Feed:gain ratio was lower in pigs fed the diets with 250 mg/kg Cu (P<0.05) than in the 10 mg/kg group. Furthermore, the abundances of NPY mRNA in hypothalamus were higher in 250 mg/kg Cu supplemented groups (P<0.05), whereas the abundances of POMC and LeptinRb mRNA were significantly reduced (P<0.05) in 170, 250 mg/kg and 100, 175, 250 mg/kg Cu supplemented groups, respectively. No difference was found for AMPKα2, MC4R, orexin, OrexinR2 mRNA expression (P>0.05). These data suggest that down-regulation of LeptinRb mRNA expression might contribute to the stimulation of feeding of high dietary Cu supplementation via regulation of NPY and POMC mRNA expression.

• Wydawca: -
• Czasopismo: Journal of Animal and Feed Sciences
• Rocznik: 2011
• Tom: 20
• Numer: 1
• Opis fizyczny: p.60-70,fig.,ref.

Twórcy

autor

Zhu D.

• Institute of Animal Nutrition, Sichuan Agricultural University, Key Laboratory of Animal Disease-resistant Nutrition, Ministry of Education, Ya'an, Sichuan, 625014, P.R. China

autor

Yu B.

autor

Ju C.

autor

Mei S.

autor

Chen D.

Bibliografia

• Adrian M.S., Staszkiewicz J., Ptitsyn A., Argyropoulos G., 2007. Circadian expression of genes regulating food intake. Obesity 15, 607-615
• Ai-Jun L., Thu T.D., Sue R., 2008. Hyperphagia and obesity produced by arcuate injection of NPY-saporin do not require upregulation of lateral hypothalamic orexigenic peptide genes. Peptides 29, 1732-1739
• Armstrong T.A., Cook D.R., Ward M.M., Williams C.M., Spears J.W., 2004. Effect of dietary copper source (cupric citrate and cupric sulfate) and concentration on growth performance and fecal copper excretion in weanling pigs. J. Anim. Sci. 82, 1234-1240
• Choi B.S., Zheng W., 2009. Copper transport to the brain by the blblood-brain barrier and blood-CSF barrier. Brain Res. 1248, 14-21
• Cromwell G.L., Stahly T.S., Monegue H.J., 1989. Effects of source and level of copper on performance and liver copper stores in weanling pigs. J. Anim. Sci, 67, 2996-3002
• Edmonds M.S., Izquierdo O.A., Baker D.H., 1985. Feed additive studies with newly weaned pigs. efficacy of supplemental copper, antibiotics, and organic acids. J. Anim. Sci. 60, 462-469
• Fakler T.M., 1999. Copper complexes improve nursery pig performance. Trace Miner Focus 5, 1-2
• Garcia de Yebenes E., Li S., Fournier A., St-Pierre S., Pelletier G., 1995. Regulation of proopiomelanocortin gene expression by neuropeptide Y in the rat arcuate nucleus. Brain Res. 674, 112-116
• Gehlert D.R., 1999. Role of hypothalamic neuropeptide Y in feeding and obesity. Neuropeptides 33, 329-338
• Li J.,Yan L., Zheng X., Liu G., Zhang N.S., Wang Z., 2008. Effect of high dietary copper on weight gain and neuropeptide Y level in the hypothalamus of pigs. J.Trace Elem. Med. Biol. 22, 33-38
• Minokoshi Y., Alquier T., Furukawa N., Kim Y.B., Lee A., Xue B., Mu J., Foufelle F., Ferré P., Birnbaum M.J., Stuck B.J., Kahn B.B., 2004. AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus. Nature 428, 569-574
• Morton G.J., Cummings D.E., Baskin D.G., Barsh G.S., Schwartz M.W., 2006. Central nervous system control of food intake and body weight. Nature 443, 289-295
• Niswender K.D., Morton G.J., Stearns W.H., Rhodes C.J., Myers M.G., Schwartz J.W., 2001. Intracellular signalling. Key enzyme in leptin-induced anorexia. Nature 413, 794-795
• Pinto S., Roseberry A.G., Liu H., Diano S., Shanabrough M., Cai X., Friedman J.M., Horvath T.L., 2004. Rapid rewiring of arcuate nucleus feeding circuits by leptin. Science 304, 110-115
• Rutkoski N.J., Fitch C.A., Yeiser E.C., Dodge J., Trombley P.Q., Levenson C.W., 1999. Regulation of neuropeptideY mRNA and peptide concentrations by copper in rat olfactory bulb. Brain Res. Mol. Brain Res. 65, 80-86
• Schwartz M.W., Woods S.C., Porte Jr. D., Seeley R.J., Baskin D.G., 2000. Central nervous system control of food intake. Nature 404, 661-671
• Seeley R.J., Yagaloff K.A., Fisher S.L., Burn P., Thiele T.E., van Dijk G., Baskin D.G., Schwartz M. W., 1997. Melanocortin receptors in leptin effects. Nature 390, 349
• Thornton J.E., Cheung C.C., Clifton D.K., Steiner R.A., 1997. Regulation of hypothalamic proopiomelanocortin mRNA by leptin in ob/ob mice. Endocrinology 138, 5063-5066
• Williamson P.S., Browning J.D., Sullivan M.J., 2002. Neuropeptide Y fails to normalize food intake in zinc-deficient rats. Nutr. Neurosci. 5, 19-25
• Yaswen L., Diehl N., Brennan M.B., Hochgeschwender U., 1999. Obesity in the mouse model of pro-opiomelanocortin deficiency responds to peripheral melanocortin. Nature Med. 5, 1066-1070

Uwagi

Rekord w opracowaniu