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2010 | 66 | 11 |

Tytuł artykułu

Bakteriologia i epizootiologia brucelozy świń

Warianty tytułu

EN
Bacteriology and epizootiology of porcine brucellosis

Języki publikacji

PL

Abstrakty

EN
The paper presents the most recent data and opinions concerning Brucella (B.) suis and porcine brucellosis. Included are up-to-date results concerning the classification of the genus Brucella into 9 species, among them B. suis. Five biovars of this species are mentioned. Biovars 1, 2 and 3 (particularly 1 and 3) are highly pathogenic for swine and for humans. The source of infection for swine are, besides swine, wild boar and hare. The global distribution of swine brucellosis is presented with particular reference to the European Union member countries. Since at present with the closed system swine production in the EU B. suis infection is not diagnosed, the main goal of veterinary services is the complex action against the introduction of B. suis into the national swine herds. The main risk factors are: importing of infected pigs from abroad and transmission of B. suis from the wild boar or the hare to the domestic swine. In order to exclude these possibilities, besides available directives and instructions, diagnostic laboratory investigations are of crucial importance. In the paper direct and indirect tests for the identification of B. suis infection are described and assessed with respect to their diagnostic value. Concerning direct diagnostic tests, besides traditional assays based on phenotypic properties of the microorganism, several genotypic methods and among them particularly the polymerase chain reaction (PCR) are characterized. Following this, indirect diagnostic methods, being serological tests used in the diagnosis of porcine brucellosis, are discussed. Their diagnostic value is evaluated, including the possibility of false positive and false negative reactions. The Brucellin allergic skin test, as a confirmatory test of the serological results, is presented. In conclusion it is stated that the present epizootic situation concerning porcine brucellosis in the EU member countries is satisfactory. In connection with this the importance of prophylactic activity of the veterinary services against transmission of B. suis infection to domestic pigs of this region is stressed.

Wydawca

-

Rocznik

Tom

66

Numer

11

Opis fizyczny

s.728-731,bibliogr.

Twórcy

  • Zakład Chorób Świń, Państwowy Instytut Weterynaryjny - Państwowy Instytut Badawczy, Al.Partyzantów 57, 24-100 Puławy
autor

Bibliografia

  • 1.Anon.: OIE, World Organization for Animal Health. Manual of diagnostic tests and vaccines for Terrestrial Animals. 6th Edition. OIE, Paris, France 2008.
  • 2.Anon.: Porcine brucellosis (Brucella suis). Scientific Opinion of the Panel on Animal Health and Welfare. The EFSA Journal 2009, 1144, 1-112.
  • 3.Bricker B. J.: PCR as a diagnostic tool for brucellosis. Vet. Microbiol. 2002, 90, 435-446.
  • 4.Chain P. S. G., Comerci D. J., Tolmasky M. E., Larimer F. W., Malftti S. A., Vergez L. M., Aguero F., Land M. L., Ugalde R. A., Garcia E.: Whole-genome analyses of speciation events in pathogenic Brucelllae. Infect. Immun. 2005, 73, 8353-8361.
  • 5.Cvetnic Z., Toncic J., Spicic S., Lojkic M., Terzic S., Jemersi L., Humski A., Curic S., Mitak M., Habrun B., Brstilo M., Ocepek M., Krt B.: Brucellosis in wild boar (Sus scrofa) in the Republic of Croatia. Veterinární Medicína 2004, 49, 115-122.
  • 6.Dedek J.: Untersuchungen zur epizootologischen Bedeutung der Hasenbrucellose. Humboldt-Universität zu Berlin 1997.
  • 7.Duggan D. J., Bittner M., Chen Y., Meltzer P., Trent J. M.: Expression profiling using cDNA microarrays. Nature Genetics 1999, 21, 10-14.
  • 8.Foster G., Osterman B. S., Godfroid J., Jacques I., Cloeckaert A.: Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hoss. International J. System. Evolution. Microbiol. 2007, 57, 2688-2693.
  • 9.Fretin D., Whatmore A. M., Al Dahouk S., Neuybauer H., Garin-Bastuji B., Albert D., Van Hessche M., Menart M., Godfroid J., Walravens K., Wattiau P.: Brucella suis identification and biovar typing by real-time PCR. Vet. Microbiol. 2008, 131, 376-385.
  • 10.Garin Bastuji B., Hars J., Thiébaud M., Artois M.: Brucellosis of domestic pigs. Reemergence of Brucella suis biovar 2 in France. Epidémiol. Santé Anim. 2000, 38, 1-5.
  • 11.Garin-Bastuji B., Zanella G., Cau C., Drapeau A., Antras V., Pozzi N.: Assessment of EIA, RBT and FPA for the diagnosis of Brucella infection in domestic pigs. Brucellosis 2008 International Research Conference (including the 61th Brucellosis Research Conference), London 10-13 September 2008.
  • 12.Godfroid J., Käsbohrer A.: Brucellosis in the European Union and Norway at the turn of the twenty-first century. Vet. Microbiol. 2002, 90, 135-145.
  • 13.Gopaul K. K., Koylass M. S., Smith C. J., Whatmore A. M.: Rapid identification of Brucella isolates to the species level by real time PCR based single nucleotide polymorphism (SNP) analysis. BMC Microbiology 2008, 8, 86.
  • 14.Kattar M. M., Jaafar R. F., Araj G. F., Le Flèche P., Matar G. M., Abi Rached R., Khalife S., Vergnaud G.: Evaluation of a multilocus variable-number tandem-repeat analysis scheme for typing human Brucella isolates in a region of brucellosis endemicity. J. Clin. Microbiol. 2008, 46, 3935-3940.
  • 15.López-Goñi I., García-Yoldi D., Marín C. M., de Miguel M. J., Muñoz P. M., Blasco J. M., Jacques I., Grayon M., Cloeckaert A., Ferreira A. C., Cardoso R., Corrêa de Sá M. I., Walravens K., Albert D., Garin-Bastuji B.: Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species and of the vaccine strains. J. Clin. Microbiol. 2008, 46, 3484-3487.
  • 16.Szulowski K., Iwaniak W., Pilaszek J., Murat J.: Wild boars and hares as reservoirs of Brucela suis biovar 2 and in Poland. Brucellosis 2008 International Research Conference (including the 61th Brucellosis Research Conference), London 10-13 September 2008.
  • 17.Szulowski K., Pilaszek J., Iwaniak W.: Bruceloza świń. Medycyna Wet. 2003, 59, 283-286.
  • 18.Truszczyński M., Wijaszka T.: Monitoring i nadzór oraz inne określenia stosowane w epidemiologii weterynaryjnej. Medycyna Wet. 2007, 63, 487-488.

Typ dokumentu

Bibliografia

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Identyfikator YADDA

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