Characterization of omega-secalin genes from rye, triticale, and a wheat 1BL/1RS translocation line

• Autorzy: Jiang Q-T. , Wei Y-M. , Andre L. , Lu Z-X. , Pu Z-E. , Peng Y-Y. , Zheng Y-L.
• Języki publikacji: EN

Abstrakty

EN

Sixty-two DNA sequences for the coding regions of omega-secalin (ω-secalin) genes have been characterized from rye (Secale cereale L.), hexaploid and octoploid triticale (× Triticosecale Wittmack), and wheat (Triticum aestivum L.) 1BL/1RS translocation line. Only 19 out of the 62 ω-secalin gene sequences were full-length open reading frames (ORFs), which can be expressed into functional proteins. The other 43 DNA sequences were pseudogenes, as their ORFs were interrupted by one or a few stop codons or frameshift mutations. The 19 ω-secalin genes have a typical primary structure, which is different from wheat gliadins. There was no cysteine residue in ω-secalin proteins, and the potential celiac disease (CD) toxic epitope (PQQP) was identified to appear frequently in the repetitive domains. The ω-secalin genes from various cereal species shared high homology in their gene sequences. The ω-secalin gene family has involved fewer variations after the integration of the rye R chromosome or whole genome into the wheat or triticale genome. The higher Ka/Ks ratio (i.e. non-synonymous to synonymous substitutions per site) in ω-secalin pseudogenes than in ω-secalin ORFs indicate that the pseudogenes may be subject to a reduced selection pressure. Based on the conserved sequences of ω-secalin genes, it will be possible to manipulate the expression of this gene family in rye, triticale, or wheat 1BL/1RS translocation lines, to reduce its negative effects on grain quality.

Słowa kluczowe

EN

grain quality; Secale cereale; storage protein; Triticum aestivum; Triticosecale; omega-secalin; 1BL-1RS translocation; rye; wheat; octoploid triticale; hexaploid triticale; gene sequence; chromosome

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2010
• Tom: 51
• Numer: 4
• Opis fizyczny: p.403-411,fig.,ref.

Twórcy

autor

Jiang Q-T.

• Triticeae Research Institute, Sichuan Agricultural University, Ya'an, Sichuan, 625014, China
• Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada
• Key Laboratory of Southwestern Crop Germplasm Utilization, Ministry of Agriculture, Ya'an, Sichuan, China

autor

Wei Y-M.

• Triticeae Research Institute, Sichuan Agricultural University, Ya'an, Sichuan, 625014, China

autor

Andre L.

• Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada

autor

Lu Z-X.

• Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada

autor

Pu Z-E.

• Triticeae Research Institute, Sichuan Agricultural University, Ya'an, Sichuan, 625014, China

autor

Peng Y-Y.

• Triticeae Research Institute, Sichuan Agricultural University, Ya'an, Sichuan, 625014, China

autor

Zheng Y-L.

• Triticeae Research Institute, Sichuan Agricultural University, Ya'an, Sichuan, 625014, China
• Key Laboratory of Southwestern Crop Germplasm Utilization, Ministry of Agriculture, Ya'an, Sichuan, China

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