Prion protein gene (PRNP) polymorphism in a flock of sheep of Kamieniecka breed

• Autorzy: Szkudlarek-Kowalczyk M. , Wisniewska E. , Milewski S. , Mroczkowski S.
• Języki publikacji: EN

Abstrakty

EN

The objective of this research was to determine allele and genotype frequencies of the prion protein gene (PRNP) in a flock of sheep and categorise thereof into groups by susceptibility to scrapie. Additionally, a mating simulation was conducted, which aimed at decreasing sheep susceptibility to scrapie. Sheep of Kamieniecka breed from a single flock were investigated. Polymorphism in the PrP gene was identified with the use of the PCR-RFLP method. Four of the five alleles were found - ARR, ARQ, ARU. and VRQ, in nine different combinations - ARR/ARR, ARR/ARQ, ARQ/ARQ, ARR/ARH, ARH/VRQ, ARR/VRQ, ARQ/ARH, ARH/ARH, and ARQ/VRQ. The most frequent allele was the one being the most desired, namely ARR, (63.2%), and the least frequent was VRQ (5.4%). Mating simulation aiming at increasing the frequency of the ARR allele in the examined sheep group demonstrated that in the F₆ generation it is possible to obtain as many as 97.7% of homozygotic animals having the lowest susceptibility to scrapie.

Słowa kluczowe

EN

prion protein gene; gene polymorphism; flock; sheep; Kamieniecka breed; polymorphism; scrapie; animal disease; veterinary medicine

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 4
• Opis fizyczny: p.645-649,ref.

Twórcy

autor

Szkudlarek-Kowalczyk M.

• Department of Genetics and General Animal Breeding, University of Technology and Life Sciences in Bydgoszcz, 85-084 Bydgoszcz, Poland

autor

Wisniewska E.

autor

Milewski S.

autor

Mroczkowski S.

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