A two-step strategy for molecular typing of multidrug-resistant Mycobacterium tuberculosis clinical isolates from Poland

• Autorzy: Jagielski T. , Augustynowicz-Kopec E. , Pawlik K. , Dziadek J. , Zwolska Z. , Bielecki J.
• Języki publikacji: EN

Abstrakty

EN

Tuberculosis (TB) continues to be one of the most challenging public health problems in the world. An important contributor to the global burden of the disease is the emergence and spread of drug-resistant and particularly multidrug-resistant Mycobacterium tuberculosis strains (MDR), defined as being resistant to at least isoniazid and rifampicin. In recent years, the introduction of different DNA-based molecular typing methods has substantially improved the knowledge of the epidemiology of TB. The purpose of this study was to employ a combination of two PCR-based genotyping methods, namely spoligotyping and IS6110-Mtb1/Mtb2 PCR to investigate the clonal relatedness of MDR M. tuberculosis clinical isolates recovered from pulmonary TB patients from Poland. Among the 50 isolates examined, 28 (56%) were clustered by spoligotyping, whereas IS6110-Mtb1/Mtb2 PCR resulted in 16 (32%) clustered isolates. The isolates that clustered in both typing methods were assumed to be clonally related. A two-step strategy consisting of spoligotyping as a first-line test, performed on the entire pool of isolates, and IS6110-Mtb1/Mtb2 PCR typing as a confirmatory subtyping method, performed only within spoligotype-defined clusters, is an efficient approach for determining clonal relatedness among M. tuberculosis clinical isolates.

Słowa kluczowe

EN

two-step strategy; molecular typing; Mycobacterium tuberculosis; clinical isolate; Poland; multidrug resistance; spoligotyping; tuberculosis; health problem; patient; public health

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2011
• Tom: 60
• Numer: 3
• Opis fizyczny: p.233-241,fig.,ref.

Twórcy

autor

Jagielski T.

• Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikova 1, 02-096 Warsaw, Poland

autor

Augustynowicz-Kopec E.

autor

Pawlik K.

autor

Dziadek J.

autor

Zwolska Z.

autor

Bielecki J.

Bibliografia

• Allix-Beguec C., M. Fauville-Dufaux and P. Supply. 2008. Threeyear population-based evaluation of standardized mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing of Mycobacterium tuberculosis. J. Clin. Microbiol. 46: 1398–1406.
• Augustynowicz-Kopeć E., M. Kozińska, A. Zabost, S. Brzezińska, M. Anielak, T. Jagielski, D. Krawiecka, R. Grzesica, W. Szymkowicz, L. Maciak and Z. Zwolska. 2007. Molecular epidemiology of pulmonary tuberculosis among closely related people (in Polish). Pneumonol. info 4: 14–22.
• Augustynowicz-Kopeć E., T. Jagielski and Z. Zwolska. 2008a. Genetic diversity of isoniazid-resistant Mycobacterium tuberculosis isolates collected in Poland and assessed by spoligotyping. J. Clin. Microbiol. 46: 4041–4044.
• Augustynowicz-Kopeć E., T. Jagielski, M. Kozińska, A. Zabost, M. Klatt and Z. Zwolska. 2008b. Molecular analysis of drug-resistant Mycobacterium tuberculosis isolates collected in central Poland. Clin. Microbiol. Infect. 14: 605–607.
• Bauer J., A.B. Andersen, K. Kremer and H. Miorner. 1999. Usefulness of spoligotyping to discriminate IS6110 low-copy-number Mycobacterium tuberculosis complex strains cultured in Denmark. J. Clin. Microbiol. 37: 2602–2606.
• Brudey K., I. Filliol, S. Ferdinand, V. Guernier, P. Duval, B. Maubert, C. Sola and N. Rastogi. 2006a. Long-term populationbased genotyping study of Mycobacterium tuberculosis complex isolates in the French departments of the Americas. J. Clin. Microbiol. 44: 183–191.
• Brudey K., J.R. Driscoll, L. Rigouts, W.M. Prodinger, A. Gori, S.A. Al-Hajoj, C. Allix, L. Aristimuño, J. Arora, V. Baumanis and others. 2006b. Mycobacterium tuberculosis complex genetic diversity: mining the fourth international spoligotyping database (SpolDB4) for classification, population genetics and epidemiology. BMC Microbiol. 6: 23.
• David S., D.R. Ribeiro, A. Antunes, C. Portugal, L. Sancho and J.G. de Sousa. 2007. Contribution of spoligotyping to the characterization of the population structure of Mycobacterium tuberculosis isolates in Portugal. Infect. Genet. Evol. 7: 609–617.
• Friedman C.R., M.Y. Stoeckle, W.D. Johnson and L.W. Riley. 1995. Double-repetitive-element PCR method for subtyping Mycobacterium tuberculosis clinical isolates. J. Clin. Microbiol. 33: 1383–1384.
• Glynn J.R., J. Bauer, A.S. de Boer, M.W. Borgdorff, P.E.M. Fine, P. Godfrey-Faussett and E. Vynnycky. 1999. Interpreting DNA fingerprint clusters of Mycobacterium tuberculosis. Int. J. Tuberc. Lung Dis. 3: 1055–1060.
• Goguet de la Salmonière Y.O., H.M. Li, G. Torrea, A. Bunschoten, J.D.A. van Embden and B. Gicquel. 1997. Evaluation of spoligotyping in a study of the transmission of Mycobacterium tuberculosis. J. Clin. Microbiol. 35: 2210–2214.
• Gori A., A. Bandera, G. Marchetti, A. Degli Esposti, L. Catozzi, G.P. Nardi, L. Gazzola, G. Ferrario, J.D.A. van Embden, D. van Soolingen and others. 2005. Spoligotyping and Mycobacterium tuberculosis. Emerg. Infect. Dis. 11: 1242–1248.
• Goyal M., N.A. Saunders, J.D.A. van Embden, D.B. Young and R.J. Shaw. 1997. Differentiation of Mycobacterium tuberculosis isolates by spoligotyping and IS6110 restriction fragment length polymorphism. J. Clin. Microbiol. 35: 647–651.
• Ijaz K., Z. Yang, H.S. Matthews, J.H. Bates and M.D. Cave. 2002. Mycobacterium tuberculosis transmission between cluster members with similar fingerprint patterns. Emerg. Infect. Dis. 8: 1257–1259.
• Kamerbeek J., L. Schouls, A. Kolk, M. van Agterveld, D. van Soolingen, S. Kuijper, A. Bunschoten, H. Molhuizen, R. Shaw, M. Goyal and J.D.A. van Embden. 1997. Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J. Clin. Microbiol. 35: 907–914.
• Kotłowski R., I.C. Shamputa, N.A. El Aila, A. Sajduda, L. Rigouts, A. van Deun and F. Portaels. 2004. PCR-based genotyping of Mycobacterium tuberculosis with new GC-rich repeated sequences and IS6110 inverted repeats used as primers. J. Clin. Microbiol. 42: 372–377.
• Kremer K., D. van Soolingen, R. Frothingham, H.W. Haas, P.W. Hermans, C. Martin, P. Palittapongarnpim, B.B. Plikaytis, L.W. Riley, M.A. Yakrus and others. 1999. Comparison of methods based on different molecular epidemiological markers for typing of Mycobacterium tuberculosis complex strains: interlaboratory study of discriminatory power and reproducibility. J. Clin. Microbiol. 37: 2607–2618.
• Lari N., L. Rindi, D. Bonanni, N. Rastogi, C. Sola, E. Tortoli and C. Garzelli. 2007. Three-year longitudinal study of genotypes of Mycobacterium tuberculosis isolates in Tuscany, Italy. J. Clin. Microbiol. 45: 1851–1857.
• Mathema B., N.E. Kurepina, P.J. Bifani and B.N. Kreiswirth. 2006. Molecular epidemiology of tuberculosis: current insights. Clin. Microbiol. Rev. 19: 658–685.
• Oelemann M.C., R. Diel, V. Vatin, W. Haas, S. Rusch-Gerdes, C. Locht, S. Niemann and P. Supply. 2007. Assessment of an optimized mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing system combined with spoligotyping for population-based molecular epidemiology studies of tuberculosis. J. Clin. Microbiol. 45: 691–697.
• Sajduda A., A. Brzostek, M. Popławska, N. Rastogi, C. Sola, E. Augustynowicz-Kopeć, Z. Zwolska, J. Dziadek and F. Portaels. 2004. Molecular epidemiology of drug-resistant Mycobacterium tuberculosis strains isolated from patients with pulmonary tuberculosis in Poland: a 1-year study. Int. J. Tuberc. Lung Dis. 8: 1448–1457.
• Sajduda A., J. Dziadek, R. Kotłowski and F. Portaels. 2006. Evaluation of multiple genetic markers for typing drug-resistant Mycobacterium tuberculosis strains from Poland. Diagn. Microbiol. Infect. Dis. 55: 59–64.
• Sola C., I. Filliol, E. Legrand, S. Lesjean, C. Locht, P. Supply and N. Rastogi. 2003. Genotyping of the Mycobacterium tuberculosis complex using MIRUs: association with VNTR and spoligotyping for molecular epidemiology and evolutionary genetics. Infect. Genet. Evol. 3: 125–133.
• Sola C., L. Horgen, J. Maisetti, A. Devallois, K.S. Goh and N. Rastogi. 1998. Spoligotyping followed by double-repetitive-element PCR as rapid alternative to IS6110 fingerprinting for epidemiological studies of tuberculosis. J. Clin. Microbiol. 36: 1122–1124.
• Supply P., E. Mazars, S. Lesjean, V. Vincent, B. Gicquel and C. Locht. 2000. Variable human minisatellite-like regions in the Mycobacterium tuberculosis genome. Mol. Microbiol. 36: 762–771.
• Supply P., S. Lesjean, E. Savine, K. Kremer, D. van Soolingen and C. Locht. 2001. Automated high-throughput genotyping for study of global epidemiology of Mycobacterium tuberculosis based on mycobacterial interspersed repetitive units. J. Clin. Microbiol. 39: 3563–3571.
• Thierry D., A. Brisson-Noël, V. Vincent-Lévy-Frébault, S. Nguyen, J.L. Guesdon and B. Gicquel. 1990. Characterization of a Mycobacterium tuberculosis insertion sequence, IS6110, and its application in diagnosis. J. Clin. Microbiol. 28: 2668–2673.
• van Embden J.D.A., M.D. Cave, J.T. Crawford, J.W. Dale, K.D. Eisenach, B. Gicquel, P. Hermans, C. Martin, R. McAdam, T.M. Schinnick and P.M. Small. 1993. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J. Clin. Microbiol. 31: 406–409.
• Vitol I., J. Driscoll, B. Kreiswirth, N. Kurepina and K.P. Bennett. 2006. Identifying Mycobacterium tuberculosis complex strain families using spoligotypes. Infect. Genet. Evol. 6: 491–504.
• World Health Organization (WHO) & International Union against Tuberculosis and Lung Disease (IUATLD). The WHO/IUATLD Global Project on Anti-Tuberculosis Drug Resistance Surveillance 2002–2007: anti-tuberculosis drug resistance in the world: 4th global report. WHO, Geneva, Switzerland, 2008. WHO/HTM/TB/2008.394.
• World Health Organization (WHO). 2006. Global tuberculosis control: surveillance, planning, financing. WHO, Geneva, Switzerland.
• Zwolska Z., E. Augustynowicz-Kopeć and M. Klatt. 2000. Primary and acquired drug resistance in Polish tuberculosis patients: results of a study of the national drug resistance surveillance programme. Int. J. Tuberc. Lung Dis. 4: 832–838.