First in vitro isolation of Neospora caninum from a naturally infected adult dairy cow in Slovakia

• Autorzy: Reiterova K. , Spilovska S. , Cobadiova A. , Mucha R.
• Języki publikacji: EN

Abstrakty

EN

The impact of in vitro isolation and molecular characterisation of Neospora caninum as well as sequence analyses was studied. The brain homogenate of a naturally Neospora-infected dairy cow (positive in ELISA and Western blot) was intraperitoneally inoculated into Mongolian gerbils (Meriones unguiculatus). The brain of gerbils on day 60 post-inoculation was homogenized, and, after trypsin-digestion, cultured on Vero cells. Neospora-like tachyzoites were first observed after 77 days of cultivation. The parasite was confirmed by polymerase chain reaction (PCR) using Neospora-specific primers Np21 and Np6. The PCR product of the first Slovak isolate (NC-SKB1) was subsequently sequenced and published in GenBank under accession number GU300774. Sequencing and BLAST search identified the isolate as N. caninum.

Słowa kluczowe

EN

Neospora caninum; infection; dairy cow; cow; brain homogenate; Mongolian gerbil; Meriones unguiculatus; brain; parasite; polymerase chain reaction; immunoblot; Slovakia

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2011
• Tom: 56
• Numer: 2
• Opis fizyczny: p.111-115,fig.,ref.

Twórcy

autor

Reiterova K.

• Parasitological Institute of the Slovak Academy of Sciences, Hlinkova 3, 040 01 Kosice, Slovak Republic

autor

Spilovska S.

autor

Cobadiova A.

autor

Mucha R.

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