Exercise-induced biochemical alterations in the blood of horses involved in recreational horseback riding in Pomeranian Region (Northern Poland)

• Autorzy: Tkachenko H. , Witaszek M. , Pazontko-Lipinski P.

Warianty tytułu

PL

Zmiany biochemiczne wywołane wysiłkiem fizycznym we krwi koni biorących udziiał w rekreacyjnej jeździe konnej w regionie pomorskim (północna Polska)

• Języki publikacji: EN

Abstrakty

EN

The aim of our study was to determine changes in some biochemical indices (alanine (ALT) and aspartate aminotransferase (AST), lactate dehydrogenase (LDH)) as well as lactate and pyruvate level in the blood of horses exercised in recreational horseback riding from Pomeranian regions during a training session. Measurement of values of liver biomarkers (AST, ALT) and muscle damage indicator (LDH), followed by a variety of training programs, can help to better understand the acute and chronic effects of resistance training. Thirteen healthy adult horses from central Pomeranian region in Poland (village Strzelinko, N54°30'48.0" E16°57'44.9"), aged 9.5±2.4 years old, were used in this study. All horses participated in recreational horseback riding. Training started at 10.00 AM, lasted 1 hour and consisted from a ride of cross country by walking (5 min), trotting (15 min), walking (10 min), trotting (10 min), walking (5 min), galloping (5 min), and walking (10 min). Blood was drawn from jugular veins of the animals in the morning, 90 minutes after feeding, while the horses were in the stables (between 8.30 and 10.00 AM), and immediately after exercise session (between 11.00 AM and 2.00 PM). Blood was stored in tubes with K3-EDTA and sodium citrate (3.8%) and held on ice until centrifugation at 3,000 rpm for 15 minutes. The plasma was removed. Plasma was used for the determination of aminotransferases and lactate dehydrogenase activity; whole blood was used for determination of lactate and pyruvate level. The regular training lead to adaptive processes which provoke changes in biochemical indices. In our research, non-significant alterations of AST and LDH activities in horses involved in recreational horseback riding were observed. This may indicate a normal course of aerobic-anaerobic glycolysis in horses involved in recreational horseback riding during a training session. Moreover, ALT activity was decreased by 20.6% (p = 0.000) during a training session. Increased blood lactate level in horses involved in recreational horseback riding during training session could be explained by increasing lactate formation via the reduction of pyruvate catalyzed by LDH as a result of anaerobic energy supply. Based on these results, it is concluded that the endurance exercises lead to specific metabolic changes accompanied by a redistribution of energy supply for improving resistance to exercises and athletic performance of horses. Therefore, the present data can be useful to assess the status of athletes and the degree of their training adaptability providing an opportunity to modify the training schedule to achieve the desired performance.

PL

Celem niniejszej pracy było określenie zmian wybranych wskaźników biochemicznych (aminotransferaza alaninowa (ALT) i asparaginianowa (AST), dehydrogenaza mleczanowa (LDH)) oraz poziomu mleczanu i pirogronianu we krwi koni z regionu Pomorza biorących udział w rekreacyjnych jazdach konnych. Pomiar wartości biomarkerów wątrobowych (AST, ALT) oraz wskaźnika uszkodzenia mięśni (LDH), a następnie różnorodne programy treningowe, mogą być pomocne w lepszym zrozumieniu ostrych i przewlekłych skutków treningu odpornościowego. W badaniach wykorzystano trzynaście zdrowych dorosłych koni z centralnego Pomorza w Polsce (wieś Strzelinko; N54°30'48,0" E16°57'44,9"), w wieku 9,5±2,4 lat. Wszystkie konie brały udział w rekreacyjnej jeździe konnej. Ćwiczenia rozpoczynały się o godz. 10 rano, trwały 1 godzinę i obejmowały: chód (5 min), kłus (15 min), chód (10 min), kłus (10 min), chód (5 min), galop (5 min) i chód (10 min). Krew od zwierząt pobierano z żył szyjnych rano, 90 minut po karmieniu (między godziną 8.30 a 10.00), podczas gdy konie były w stajni, oraz natychmiast po treningu (między godziną 11.00 a 14.00). Krew przechowywano w probówkach z K3-EDTA i cytrynianem sodu (3,8%) na lodzie do momentu odwirowania przy 3000 obrotów przez 15 minut. Osocze zostało zlane. Osocze stosowano do oznaczenia aminotransferaz i aktywności dehydrogenazy mleczanowej; do oznaczania poziomu mleczanu i pirogronianu zastosowano pełną krew. Regularne treningi prowadzą do procesów adaptacyjnych, które prowokują do zmian wskaźników biochemicznych. W naszych badaniach zaobserwowano nieistotne zmiany aktywności AST i LDH u koni uczestniczących w rekreacyjnej jeździe konnej. Może to wskazywać na normalny przebieg tlenowej/beztlenowej glikolizy u koni podczas treningu. Ponadto aktywność ALT została zmniejszona o 20,6% (p = 0,000) podczas sesji treningowej. Zwiększony poziom mleczanu we krwi u koni biorących udział w rekreacyjnej jeździe konnej można wytłumaczyć wzmożonym wytwarzaniem mleczanu poprzez redukcję pirogronianu katalizowanego przez LDH w efekcie beztlenowego dostarczania energii. Na podstawie naszych wyników można stwierdzić, że ćwiczenia wytrzymałościowe prowadzą do określonych zmian metabolicznych, którym towarzyszy redystrybucja zaopatrzenia w energię w celu poprawy odporności na ćwiczenia i wyniki sportowe koni. Dlatego też niniejsze dane mogą być przydatne w ocenie statusu koni sportowych i stopnia ich zdolności adaptacyjnych do treningów, dając możliwość modyfikacji harmonogramu treningu, by osiągnąć pożądaną wydajność.

Słowa kluczowe

EN

Pomeranian region; recreational riding; horseback riding; horse; blood; biochemical alteration; biochemical index; aminotransferase; alanine aminotransferase; aspartate aminotransferase; lactate dehydrogenase; lactate; pyruvate

• Wydawca: -
• Czasopismo: Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline
• Rocznik: 2018
• Tom: 22
• Opis fizyczny: p.91-108,fig.,ref.

Twórcy

autor

Tkachenko H.

• Institute of Biology and Environmental Protection, Pomeranian University in Slupsk, ul. Arciszewskiego 22B, 76-200 Slupsk, Poland

autor

Witaszek M.

• Institute of Biology and Environmental Protection, Pomeranian University in Slupsk, ul. Arciszewskiego 22B, 76-200 Slupsk, Poland

autor

Pazontko-Lipinski P.

• Institute of Biology and Environmental Protection, Pomeranian University in Slupsk, ul. Arciszewskiego 22B, 76-200 Slupsk, Poland

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