Influence of thidiazuron (TDZ) pretreatment of shoot tips on shoot multiplication and ex vitro acclimatization of Harpagophytum procumbens

• Autorzy: Grabkowska R. , Sitarek P. , Wysokinska H.
• Języki publikacji: EN

Abstrakty

EN

The effects of thidiazuron (TDZ) pretreatment of shoot tips on Harpagophytum procumbens shoot proliferation and successive stages of micropropagation, i.e. rooting of regenerated shoots and acclimatization of plantlets to ex vitro conditions, were described in the present study. The best response in terms of shoot proliferation (about seven shoots/explant) and shoot length (3.2 ± 0.4 cm) was obtained when explants pretreated with 25 μmol L⁻¹ TDZ for 6 h were cultured on Schenk and Hildebrandt medium containing indole-3-acetic acid (IAA) (0.57 μmol L⁻¹) and 6-benzylaminopurine (BAP) (8 μmol L⁻¹). Under these conditions, a 330 % increase in shoot multiplication over TDZ non-pretreatment culture was achieved and TDZ pretreatment shoots were longer compared to those in control culture (2.6 ± 0.3 cm). The TDZ pretreatment did not affect the percentage of rooted shoots, length of roots and number of roots formed per shoot. The rooted plantlets were transplanted from in vitro to pots with soil and grown during 1 year in the greenhouse. The hardening process was difficult and time-consuming. We found that the plants developed from the TDZ pretreated culture were superior to plants from non-pretreated culture in terms of survival rate and morphological features, such as shoot length, leaf size, flowering and earlier root tuberisation. Random amplified polymorphic DNA and inter-simple sequence repeat analyses of pretreatment with TDZ plants showed genetic similarity to non-pretreatment plants. We conclude that applying the strategy of initial explant pretreatment with TDZ may be valuable for the improvement in H. procumbens in vitro propagation.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 07
• Opis fizyczny: p.1661-1672,fig.,ref.

Twórcy

autor

Grabkowska R.

• Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland

autor

Sitarek P.

• Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland

autor

Wysokinska H.

• Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland

Bibliografia

• Bairu MW, Jain N, Stirk WA, Doležal K, Van Staden J (2009) Solving the problem of shoot-tips necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium. South Afr J Bot 75:122–127. doi:10.1016/j.sajb.2008.08.006
• Boje K, Lechtenberg M, Nahrstedt A (2003) New and known iridoidand phenylethanoid glycosides from Harpagophytum procumbens and their in vitro inhibition of human leukocyte elastase. Planta Med 69:820–825. doi:10.1055/s-2003-43225
• Burger JFW, Brandt EV, Ferreira D (1987) Iridoid and phenolic glycosides from Harpagophytum procumbens. Phytochemistry 26:1453–1457
• Cruz de Carvalho MH, Van Le B, Zuily-Fodil Y, Thi ATP, Van KTT (2000) Efficient whole plant regeneration of common bean (Phaseolus vulgaris L.) using thin-cell-layer culture and silver nitrate. Plant Sci 159:223–232
• ESCOP (European Scientific Cooperative on Phytotherapy) Monographs Harpagophyti radix (2003) 2nd edn. Thieme Verlag, Stuttgart, pp 233–240
• Estrada-Luna AA, Davies FT, Egilla JN (2001) Physiological changes and growth of micropropagated chile ancho pepper plantlets during acclimatization and post-acclimatization. Plant Cell Tissue Org Cult 66:17–24
• European Pharmacopoeia (2010) 7th edn. vol 2. Council of Europe, Strasbourg
• Grąbkowska R, Wysokińska H (2009) Micropropagation of Harpagophytum procumbens (Burch.) DC. ex Meisn.; the effect of cytokinins on shoot multiplication. Herba Polonica 55:244–250
• Hachfeld B, Schippmann U (2000) Conservation data sheet 2: exploitation, trade and population status of Harpagophytum procumbens in southern Africa. Med Plant Conserv 6:4–9
• Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Org Cult 33:105–119
• Isutsa DK (2004) Rapid micropropagation of passion fruit (Passiflora edulis Sims.) varieties. Sci Hortic 99:395–400. doi:10.1016/j.scienta.2003.08.002
• Jahan AA, Anis M, Aref IM (2011) Preconditioning of axillary buds in thidiazuron-supplemented liquid media improves in vitro shoot multiplication in Nyctanthes arbor-tristis L. Appl Biochem Biotech 163:851–859. doi:10.1007/s12010-010-9089-7
• Jain N, Bairu WM, Stirk WA, Van Staden J (2009) The effect of medium, carbon source and explant on regeneration and control of shoot-tip necrosis in Harpagophytum procumbens. South Afr J Bot 75:117–121. doi:10.1016/j.sajb.2008.08.005
• Kaliamoorthy S, Naidoo G, Achar P (2008) Micropropagation of Harpagophytum procumbens. Biol Plant 52:191–194
• Kozak D (2010) The effect of 6-benzylaminopurine, thidiazuron and the type of explants on in vitro propagation of Yucca elephantipes Regel. Acta Sci Pol Hortorum Cultus 9:211–219
• Kucharska D, Orlikowska T (2009) Enhancement of in vitro organogenetic capacity of rose by preculture of donor shoots on the medium with thidiazuron. Acta Physiol Plant 31:495–500. doi:10.1007/s11738-008-0258-2
• Levieille G, Wilson G (2002) In vitro propagation and iridoid analysis of the medicinal species Harpagophytum procumbens and H. zeyheri. Plant Cell Rep 21:220–225. doi:10.1007/s00299-002-0520-6
• Lin CH-S, Liang CJ, Hsaio HW, Lin MJ, Chang WC (2007) In vitro flowering of green and albino Dendrocalamus latiflorus. New For 34:177–186. doi:10.1007/s11056-007-9045-8
• Malik KA, Saxena PK (1992) Regeneration in Phaseolus vulgaris L.: high-frequency induction of direct shoot formation in intact seedlings by N⁶-benzylaminopurine and thidiazuron. Planta 186:384–389
• Medeiros LA, de Ribeiro RCS, Gallo LA, de Oliveira ET, Demattê ME (2006) In vitro propagation of Notocactus magnificus. Plant Cell Tissue Org Cult 84:165–169. doi:10.1007/s11240-005-9014-x
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497. doi:10.1111/ppl.1962.15.issue-3/issutoc
• Prathanturarug S, Soonthornchareonnon N, Chuakul W, Phaidee Y, Saralamp P (2003) High-frequency shoot multiplication in Curcuma longa L. using thidiazuron. Plant Cell Rep 21:1054–1059. doi:10.1007/s00299-003-0629-2
• Prathanturarug S, Soonthornchareonnon N, Chuakul W, Phaidee Y, Saralamp P (2005) Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron-supplemented liquid medium. Plant Cell Tissue Org Cult 80:347–351
• Pryce S, Lumsden PJ, Berger F, Leifert C (1993) Effect of plant density and macronutrient nutrition on Delphinium shoot cultures. J Hortic Sci 68:807–813
• Qi J, Chen J-J, Cheng Z-H, Zhou J-H, Yu B-Y, Qiu SX (2006) Iridoid glycosides from Harpagophytum procumbens D.C. (devil’s claw). Phytochemistry 67:1372–1377. doi:10.1016/j.phytochem.2006.05.029
• Raimondo D, Donaldson J (2002) The trade, management and biological status of Harpagophytum spp. in Southern African range states. In: 12th meeting of the Plants Committee, Leiden, pp 1–20
• Ramanayake SMSD, Meemaduma VN, Weerawardene TE (2006) In vitro shoot proliferation and enhancement of rooting for the large-scale propagation of yellow bamboo (Bambusa vulgaris ‘Striata’). Sci Hortic 110:109–113. doi:10.1016/j.scientia.2006.06.016
• Rewers M, Kisiala A, Drouin J, Śliwińska E, Cholewa E (2012) In vitro-regenerated wetland sedge Eriophorum vaginatum L. is genetically stable. Acta Physiol Plant 34:2197–2206. doi:10.1007/s11738-012-1020-0
• Schenk RW, Hildebrandt AC (1972) Medium and techniques for induction of growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:199–204
• Shushu DD (2001) In vitro regeneration of the Kalahari devil’s claw, Harpagophytum procumbens, an important medicinal plant. South Afr J Bot 67:378–380
• Siddique I, Anis M (2007) Rapid micropropagation of Ocimum basilicum using shoot tip explants pre-cultured in thidiazuron supplemented liquid medium. Biol Plant 51:787–790
• Singh SK, Syamal MM (2001) A short pre-culture soak in thidiazuron or forchlorfenuron improves axillary shoot proliferation in rose micropropagation. Sci Hortic 91:169–177
• Singh M, Jaiswal U, Jaiswal VS (2000) Thidiazuron-induced in vitro flowering in Dendrocalamus strictus Nees. Curr Sci 79:1529–1530
• Singh M, Jaiswal U, Jaiswal VS (2001) Thidiazuron-induced shoot multiplication and plant regeneration in bamboo (Dendrocalamus strictus Nees). J Plant Biochem Biotechnol 10:133–137
• Song G-Q, Sink KC (2005) Optimizing shoot regeneration and transient expression factors for Agrobacterium tumefaciens transformation of sour cherry (Prunus cerasus L.) cultivar Montmorency. Sci Hortic 106:60–69. doi:10.1016/j.scientia.2005.02.018
• Thomas TD (2007) Pretreatment in thidiazuron improves the in vitro shoot induction from leaves in Curculigo orchioides Gaertn., an endangered medicinal plant. Acta Physiol Plant 29:455–461. doi:10.1007/s11738-007-0055-0
• Wichtl M (2004) Herbal drugs and phytopharmaceuticals. Medpharm Scientific Publishers, Stuttgart, pp 271–273

Identyfikatory

DOI

10.1007/s11738-014-1541-9