Cloning, expression, and evolutionary analysis of alpha-gliadin genes from Triticum and Aegilops genomes

• Autorzy: Li J. , Wang S.-L. , Cao M. , Lv D.-W. , Subburaj S. , Li X.-H. , Zeller F. J. , Hsam S. L. K. , Yan Y.-M.
• Języki publikacji: EN

Abstrakty

EN

Fifteen novel α-gliadin genes were cloned and sequenced from Triticum and related Aegilops genomes by allele-specific polymerase chain reaction (AS-PCR). Sequence comparison displayed high diversities in the α-gliadin gene family. Four toxic epitopes and glutamine residues in the two polyglutamine domains facilitated these α-gliadins to be assigned to specific chromosomes. Five representative α-gliadin genes were successfully expressed in Escherichia coli, and their amount reached a maximum after 4 h induced by isopropyl-β-D-thiogalactoside (IPTG), indicating a high level of expression under the control of T7 promoter. The transcriptional expression of α-gliadin genes during grain development detected by quantitative real-time polymerase chain reaction (qRT-PCR) showed a similar up– down regulation pattern in different genotypes. A neighborjoining tree constructed with both full-open reading frame (ORF) α-gliadin genes and pseudogenes further revealed the origin and phylogenetic relationships among Triticum and related Aegilops genomes. The evolutionary analysis demonstrated that α-gliadin genes evolved mainly by synonymous substitutions under strong purifying selection during the evolutionary process.

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2013
• Tom: 54
• Numer: 2
• Opis fizyczny: p.157–167,fig.,ref.

Twórcy

autor

Li J.

• College of Life Science, Capital Normal University, 100048 Beijing, China

autor

Wang S.-L.

autor

Cao M.

autor

Lv D.-W.

autor

Subburaj S.

autor

Li X.-H.

autor

Zeller F. J.

autor

Hsam S. L. K.

autor

Yan Y.-M.

Uwagi

PL

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