Genome size and phenotypic variation of Nymphaea (Nymphaeaceae) species from Eastern Europe and temperate Asia

• Autorzy: Dabrowska M.A. , Rola K. , Volkova P. , Suda J. , Zalewska-Galosz J.
• Języki publikacji: EN

Abstrakty

EN

Despite long-term research, the aquatic genus Nymphaea still possesses major taxonomie challenges. High phenotypic plasticity and possible interspecific hybridization often make it impossible to identify individual specimens. The main aim of this study was to assess phenotypic variation in Nymphaea taxa sampled over a wide area of Eastern Europe and temperate Asia. Samples were identified based on species-specific genome sizes and diagnostic morphological characters for each taxon were then selected. A total of 353 specimens from 32 populations in Poland, Russia and Ukraine were studied, with nine biometric traits being examined. Although some specimens morphologically matched N. xborealis (a hybrid between N. alba and N. Candida) according to published determination keys, only one hybrid individual was revealed based on genome size data. Other specimens with intermediate morphology possessed genome size corresponding to N. alba, N. Candida or N. tetragona. This indicates that natural hybridization between N. alba and N. Candida is not as frequent as previously suggested. Our results also revealed a considerably higher variation in the studied morphological traits (especially the quantitative ones) in N. alba and N. Candida than reported in the literature. A determination key for the investigated Nymphaea species is provided, based on taxonomically-informative morphological characters identified in our study.

Słowa kluczowe

EN

genome size; phenotypic variation; Nymphaea alba; Nymphaea candida; Nymphaea tetragona; Nymphaea x borealis; Nymphaeaceae; morphometric analysis; flow cytometry; interspecies hybridization; Eastern Europe; temperate region; Asia

• Wydawca: -
• Czasopismo: Acta Societatis Botanicorum Poloniae
• Rocznik: 2015
• Tom: 84
• Numer: 2
• Opis fizyczny: p.277-286,fig.,ref.

Twórcy

autor

Dabrowska M.A.

• Department of Plant Taxonomy, Phytogeography and Herbarium, Institute of Botany, Jagiellonian University, Kopernika 27, 31-501 Krakow, Poland

autor

Rola K.

• Department of Plant Taxonomy, Phytogeography and Herbarium, Institute of Botany, Jagiellonian University, Kopernika 27, 31-501 Krakow, Poland

autor

Volkova P.

• Department of Higher Plants, Faculty of Biology, Moscow State University, Vorobyevy Gory, 119899 Moscow, Russian Federation

autor

Suda J.

• Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, 128 01 Prague, Czech Republic
• Institute of Botany, The Czech Academy of Sciences, Zamek 1, 252 43 Pruhonice, Czech Republic

autor

Zalewska-Galosz J.

• Department of Plant Taxonomy, Phytogeography and Herbarium, Institute of Botany, Jagiellonian University, Kopernika 27, 31-501 Krakow, Poland

Bibliografia

• 1.Borsch T, Hilu KW, Wiersema JH, Löhne C, Barthlott W, Wilde V. Phylogeny of Nymphaea (Nymphaeaceae): evidence from substitutions and microstructural changes in the chloroplast trnT-trnF region. Int J Plant Sci. 2007;168(5):639-671. http://dx.doi.org/10.1086/513476
• 2.Gupta PP. Cytogenetics of aquatic ornamentals. VI. Evolutionary trends and relationships in the genus Nymphaea. Cytologia. 1980;45:307-314. http://dx.doi.org/10.1508/cytologia.45.307
• 3.Borsch T, Löhne C, Mbaye MS, Wiersema J. Towards a complete species tree of Nymphaea: shedding further light on subg. Brachy-ceras and its relationships to the Australian water-lilies. Telopea. 2011;13(1-2):193-217.
• 4.Hultén E, Fries M. Atlas of North European vascular plants. North of the tropic of cancer. Vol. I. Königstein: Koeltz Scientific Books, Federal Republic of Germany; 1986.
• 5.Komarov VL. Nymphaea L. In: Komarov VL, editor. Flora of the USSR. Jeruzalem: Israel Program for Scientific 19 Translators; 1970. p. 8-12. (vol 7).
• 6.Tutin TG, Webb DA. Nymphaea. In: Tutin TG, Burges NA, Chater AO, Edmondson JR, Heywood VH, Moore DM, et al., editors. Flora Europaea I. 2nd ed. Cambridge: Cambridge University Press; 1993. p. 246-247.
• 7.Zając M, Zając A. The geographical elements of native flora of Poland. Kraków: Drukarnia Uniwersytetu Jagiellońskiego; 2009.
• 8.Volkova PA, Trávníček P, Brochmann C. Evolutionary dynamics across discontinuous freshwater systems: Rapid expansions and repeated allopolyploid origins in the Palearctic white water-lilies (Nymphaea). Taxon. 2010;59:483-494.
• 9.Rothmaler W. Exkursionsflora. Kritischer Band. Berlin: Volk und Wissen Volkseigener Verlag Berlin; 1976.
• 10.Tomšovic P. Nymphaeaceae. In: Hejný S, Slavík B, editors. Květena České socialistické republiky. Praha: Academia; 1988. p. 355-359.
• 11.Szańkowski M, Kłosowski S. Habitat conditions of nymphaeid associations in Poland. Hydrobiologia. 1999;415:177-185. http://dx.doi. org/10.1007/978-94-017-0922-4_25
• 12.Gąbka M. Habitat requirements of nymphaeids in humic lakes of the Wielkopolska region (western Poland). Biodivers Res Conserv. 2006;3-4:357-360.
• 13.Neuhäusl R, Tomšovic P. Rod Nymphaea (L.) Smith v Čechoslovensku. Preslia. 1957;29:225-249.
• 14.Jasiewicz A. Flora Polski. Rośliny naczyniowe. Warszawa: Państwowe Wydawnictwo Naukowe; 1985. (vol 4).
• 15.Szafer W, Kulczyński S, Pawłowski B. Rośliny polskie. Część I. Warszawa: Państwowe Wydawnictwo Naukowe; 1988.
• 16.Kubát K. Klíč ke květně České republiky. Praha: Academia; 2002.
• 17.Rutkowski L. Klucz do oznaczania roślin naczyniowych Polski niżowej. Warszawa: Wydawnictwo Naukowe PWN; 1998.
• 18.Werner K, Hellwig F. Hybridization between Nymphaea alba and Nymphaea candida investigated by AFLP fingerprinting and morphological data. In: Abstracts of 17th symposium "Biodiversity and evolutionary biology". Bonn; 2006. p. 227.
• 19.Volkova PA, Shipunov AB. Morphological variation of Nymphaea (Nymphaeaceae) in European Russia. Nord J Bot. 2007;25:329-338. http://dx.doi.org/10.1111/j.0107-055X.2007.00140.x
• 20.Kabátová K, Vít P, Suda J. Species boundaries and hybridization in central-European Nymphaea species inferred from genome size and morphometric data. Preslia. 2014;86:131-154.
• 21.Caspary R. Neue und seltene Pflanzen Preussens, gefunden 1870. Schriften der Köniüichen Physikalisch-Ökonomischen Gesellschaft zu Königsberg. 1871:61-64.
• 22.Camus EG. Statistique ou catalogue des plantes hybrids spontanées de la flore européenne. Journal de Botanique. 1898;12:97-103.
• 23.Gąbka M, Owsianny PM, Sobczyński T. Acid lakes in the Wielkopolska region - physic-chemical properties of water, bottom sediments and the aquatic micro- and macrovegetation. Limnological Review. 2004;4:81-88.
• 24.Ejankowski W, Małysz B. Morphological variability of the water lily (Nymphaea) in the Polesie Zachodnie region, Eastern Poland. Biologia. 2011;66(4):604-609. http://dx.doi.org/10.2478/s11756-011-0055-9
• 25.Ciecierska H. Makrofity jako wskaźniki stanu ekologicznego jezior. Rozprawy i monografie. Olsztyn: Wydawnictwo Uniwersytetu Warmińsko-Mazurskiego; 2008.
• 26.Muntendam JB, Povel GDE, van der Velde G. Morphometric patterns in the Nymphaea alba-candida complex. Acta Botanica Neerlandica. 1996;45(3):279-302. http://dx.doi.org/10.1111/j.1438-8677.1996. tb00517.x
• 27.Nowak A, Nowak S. The problem of the occurrence of Nymphaea candida C. Presl in the Opole Silesia. Nat J (Opole). 2007;40:25-33.
• 28.Conrad HS. The waterlilies: a monograph of the genus Nymphaea. Washington, DC: Carnegie Institution of Washington; 1905. http:// dx.doi.org/10.5962/bhl.title.23557
• 29.Heslop-Harrison Y. Nymphaea L. em. Sm. (nom. conserv.). J Ecol. 1955;43:719-734. http://dx.doi.org/10.2307/2257032
• 30.Les DH, Philbrick CT. Studies of hybridization and chromosome number variation in aquatic plants: evolutionary implications. Aquat Bot. 1993;44:181-228. http://dx.doi.org/10.1016/0304-3770(93)90071-4
• 31.Wayda M. The distribution of Nymphaea candida C. Presl (Nymphaeaceae) in Poland. Acta Soc Bot Pol. 2000;69(1):75-79. http://dx.doi. org/10.5586/asbp.2000.009
• 32.Nowak A, Nobis M, Dajdok Z, Zalewska-Gałosz J, Nowak S, Nobis A, et al. Revision of Nymphaea candida range - new data on the distribution and habitat preferences of the species in southern Poland. Acta Soc Bot Pol. 2010;79(4):333-350. http://dx.doi.org/10.5586/asbp.2010.041
• 33.Otto F. DAPI staining of fixed cells for high-resolution flow cytometry of nuclear DNA. In: Crissman HA, Darzynkiewicz Z, editors. Methods in cell biology. New York, NY: Academic Press; 1990. p: 105-110. (vol 33). http://dx.doi.org/10.1016/S0091-679X(08)60516-6
• 34.Suda J, Trávníček P. Reliable DNA ploidy determination in dehydrated tissues of vascular plants by DAPI flow cytometry: new prospects for plant research. Cytometry A. 2006;69A(4):273-280. http://dx.doi. org/10.1002/cyto.a.20253
• 35.Doležel J, Sgorbati S, Lucretti S. Comparison of three DNA fluo-rochromes for flow cytometric estimation of nuclear DNA content in plants. J Plant Physiol. 1992;85:625-631. http://dx.doi. org/10.1111/j.1399-3054.1992.tb04764.x
• 36.Sokal RR, Sneath PH. Principles of numerical taxonomy. San Francisco, CA: W.H. Freeman; 1963.
• 37.Sneath PH, Sokal RR. Numerical taxonomy. San Francisco, CA: W.H. Freeman; 1973.
• 38.Cattell RB. The scree test for the number of factors. Multivariate Behav Res. 1966;1:245-276. http://dx.doi.org/10.1207/s15327906mbr0102_10
• 39.Gower JC. A general coefficient of similarity and some of its properties. Biometrics. 1971;27:857-874. http://dx.doi.org/10.2307/2528823
• 40.Marhold K. Multivariate morphometrics and its application to monography at specific and infraspecific levels. In: Stuessy TF, Lack HW, editors. Monographic plant systematics: fundamental assessment of plant biodiversity. Ruggell: Gantner; 2011. p. 73-99.
• 41.Kovach WL. MVSP - A Multivariate Statistical Package for Windows, ver. 3.1. Pentraeth: Kovach Computing Services; 1999.
• 42.Glück H. Biologische und morphologische Untersuchungen über Wasser-und Sumpfgewächse. Vierter Teil: Untergetauchte und Nymphaea hybrids is masking the decline of wild-type Nymphaea Schwimmblattflora. Jem: G. Р^Лег; 1924. alba in Hesse, Germany. Flora. 2014;209(2):122-130. http://dx.doi.
• 43. Nierbauer KU, Kanz B, Zizka G. The widespread naturalisation of org/10.1016/j.flora.2013.12.005

Identyfikatory

DOI

10.5586/asbp.2015.016