The effect of Fe-EDDHA on shoot multiplication and in vitro rooting of Carlina onopordifolia Besser

• Autorzy: Trejgell A. , Libront I. , Tretyn A.
• Języki publikacji: EN

Abstrakty

EN

The effect of two different iron chelates and iron concentration on multiplication, shoot growth, chlorophyll content and rooting of Carlina onopordifolia were studied in in vitro culture. FeEDTA presented in MS basal medium was replaced by FeEDDHA, which was applied in three concentrations: 93.5, 187.0 and 280.5 mg dm⁻³ (5.6 mg dm⁻³, 11.2 and 16.8 mg dm⁻³ Fe ions, respectively). Changing chelate or iron concentration in the medium had no effect on axillary shoot number proliferation, but growth of shoots was significantly inhibited by a two- and three-fold increase in concentration of FeEDDHA in the medium. Supplementation of the medium with FeEDDHA as Fe source significantly increased the level of chlorophyll in the leaves. After treatment of shoots with IBA for 5 s and growing them on the MS medium supplemented with FeEDTA, the number of roots per shoot was significantly higher than on medium containing FeEDDHA. Increasing the concentration of Fe ions in the medium after a short pulse (5 s) of IBA had no effect on shoot rooting. After 30 s of 1-g dm⁻³ IBA treatment, growth of roots on medium with FeEDDHA was stimulated. The survival rate was relatively low and did not depend on the type and concentration of iron chelate in the rooting medium.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 5
• Opis fizyczny: p.2051-2055,fig.,ref.

Twórcy

autor

Trejgell A.

• Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Gagarina 9, 87-100 Torun, Poland

autor

Libront I.

• Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Gagarina 9, 87-100 Torun, Poland

autor

Tretyn A.

• Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Gagarina 9, 87-100 Torun, Poland

Bibliografia

• Antonopoulos C, Dimassi K, Therios I, Chatzissavvidis C, Papadakis I (2007) The effect of Fe–EDDHA and of ascorbic acid on in vitro rooting of the peach rootstock GF-677 explants. Acta Physiol Plant 29:559–561
• Arnon DI (1949) Copper enzymes in isolated chloroplasts polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–14
• Castillo B, Smith MAL, Madhavi DL (1997) Interactions of irradiance level and iron chelate source during shoot tip culture of Carica papaya L. Hort Science 32:1120–1123
• Christensen B, Sriskandarajah S, Serek M, Müller R (2008) In vitro culture of Hibiscus rosa-sinensis L.: Influence of iron, calcium and BAP on establishment and multiplication. Plant Cell Tiss Org Cult 93:151–161
• Curie C, Cassin G, Couch D, Divol F, Higuchi K, Le Jean M, Misson J, Schikora A, Czernic P, Mari S (2009) Metal movement within the plant: contribution of nicotianamine and yellow stripe 1-like transporters. Ann Bot 103:1–11
• Dalton CC, Iqbal K, Turver DA (1983) Iron phosphate precipitation in Murashige and Skoog media. Physiol Plant 57:472–476
• Dimassi K, Chouliaras V, Diamantidis G, Therios I (2003) Effect of iron and auxin on peroxidase activity and rooting performance of three Citrus rootstocks in vitro. J Plant Nutr 26:1023–1034
• Gaspar T, Kevers C, Hausman JF, Berthon JY, Ripetti V (1992) Practical use of peroxidase activity as a predictive marker of rooting performance of micropropagated shoots. Agronomie 12:757–765
• Gómez-Gallego M, Pellico D, Ramírez-López P, Mancheño MJ, Romano S, de la Torre MC, Sierra MA (2005) Understanding of the mode of action of FeIII-EDDHA as iron chlorosis corrector based on its photochemical and redox behavior. Chem Eur J 11:5997–6005
• Grubišić D, Šavikin-Fodulović K, Mišić D, Giba Z, Konjević R (2004) In vitro stem elongation of stemless carline thistle. Plant Growth Regul 44:65–69
• Guerinot ML (2001) Improving rice yields—ironing out the details. Nature Biotech 19:417–418
• Hazarika BN (2006) Morpho-physiological disorders in in vitro culture of plants. Sci Hort 108:105–120
• Lombardi L, Sebastiani L, Vitagliano C (2003) Physiological, biochemical, and molecular effects of in vitro induced iron deficiency in peach rootstock Mr.S 2/5. J Plant Nutr 26:2149–2163
• Marchant S, Dreyfuss BW (1998) Post-translational assembly of photosynthetic metalloproteins. Annu Rev Plant Physiol Plant Mol Biol 49:25–51
• Molassiotis AN, Dimassi K, Therios I, Diamantidis G (2003/2004) Fe-EDDHA promotes rooting of rootstock GF-677 (Prunus amygdalus 9 P. persica) explants in vitro. Biol Plant 1:141–144
• Molassiotis AN, Dimassi K, Diamantidis G, Therios I (2004) Changes in peroxidases and catalase activity during in vitro rooting. Biol Plant 48:1–5
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:437–497
• Nenova VR (2009) Growth and photosynthesis of pea plants under different iron supply. Acta Physiol Plant 31:385–391
• Ramage CM, Williams RR (2003) Mineral uptake in tobacco leaf discs during different developmental stages of shoot organogenesis. Plant Cell Rep 21:1047–1053
• Romera FJ, Alcantara E, De La Guardia MD (1999) Ethylene production by Fe-deficient root and its involvement in the regulation of Fe-deficiency stress responses by strategy I plants. Ann Bot 83:51–55
• Schwambach J, Fadanelli C, Fett-Neto (2005) Mineral nutrition and adventitious rooting in microcuttings of Eucalyptus globulus. Tree Physiol 25:487–494
• Shibli RA, Mohammad MJ, Ajlouni ZI (2002) Growth and micronutrient acquisition of in vitro grown bitter almond and sour orange in response to iron concentration from different iron chelates. J Plant Nutr 25(7):1599–1606
• Stasinopoulos TC, Hangarter RP (1990) Preventing photochemistry in culture media by long-pass light filters alters growth of cultured tissue. Plant Physiol 93:1365–1369
• Thomas P, Mythili Jb, Shivashankara KS (2000) Effects of photooxidative loss of FeNa2EDTA and of higher iron supply on chlorophyll content, growth and propagation rate in triploid watermelon cultures. In Vitro Cell Dev Biol Plant 36:537–542
• Trejgell A, Tretyn A (2011) Shoot multiplication and in vitro rooting of Carlina onopordifolia Basser. Acta Biol Cracov 53(2):68–72
• Trejgell A, Bednarek M, Tretyn A (2009a) Micropropagation of Carlina acaulis L. Acta Biol Cracov 51:97–103
• Trejgell A, Da˛browska G, Tretyn A (2009b) In vitro regeneration of Carlina acaulis subsp. Simplex from seedling explants. Acta Physiol Plant 31:445–453
• Tsao WV, Reed BM (2002) Gelling agents, silver nitrate, and sequestrene iron influence adventitious shoot and callus formation from Rubus leaves. In vitro Cell Dev Biol Plant 38:29–32
• Van der Salm TPM, Van der Toorn CJG, Hanish ten Cate CH, Dubois LAM, De Vries DP, Dons HJM (1994) Importance of the iron chelate for micropropagation of Rosa hybrida L. ‘Moneyway’. Plant Cell Tiss Org Cult 37:73–77
• Zawadzka M, Orlikowska T (2006a) Factors modifying regeneration in vitro of adventitious shoots in five red raspberry cultivars. J Fruit Ornam Plant Res 14:105–115
• Zawadzka M, Orlikowska T (2006b) The influence of Fe-EDDHA in red raspberry cultures during shoot multiplication and adventitious regeneration from leaf explants. Plant Cell Tiss Org Cult 85:145–149

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