Reproducibility of two-dimensional electrophoresis gel images of pea (Pisum sativum L.) seed proteins evaluated using scatter plots - a short report

• Autorzy: Dziuba J. , Minkiewicz P. , Nalecz D. , Dziuba M. , Szerszunowicz I.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to evaluate plots showing a linear correlation between the parameters characterising protein content in the spots obtained using two-dimensional electrophoresis (scatter plots). The correlation coefficient of normalized spot volume may be used to determine regions with increased reproducibility. In our experiment, conducted using pea seed proteins, the correlation coefficient reached 0.980 in such regions, compared with 0.917 noted for the entire gel surface. The correlation coefficient calculated based on scatter plots may serve as a tool for the preliminary selection of regions characteristic of a given proteome.

• Wydawca: -
• Czasopismo: Polish Journal of Food and Nutrition Sciences
• Rocznik: 2009
• Tom: 59
• Numer: 2
• Opis fizyczny: p.141-144,fig.,ref.

Twórcy

autor

Dziuba J.

• Chair of Food Biochemistry, University of Warmia and Mazury in Olsztyn, Plac Cieszynski 1, 10-726 Olsztyn-Kortowo, Poland

autor

Minkiewicz P.

autor

Nalecz D.

autor

Dziuba M.

autor

Szerszunowicz I.

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Uwagi

PL

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