Expression and immunogenicity study of a novel mhp183 gene fragment of Mycoplasma hyopneumoniae

• Autorzy: Xu M. , Zheng J. , Hu S. , Wang G.
• Języki publikacji: EN

Abstrakty

EN

A highly immunogenic nucleotide fragment (195bp) was selected from the Mhp183 gene of Mycoplasma hyopneumoniae using information software technology and was named Mhp183195bp. Three Mhp183195bp were linked to form a new nucleotide sequence called Mhp183615bp. Mhp183615bp was directly synthesized and cloned into a pET100 vector and expressed in Escherichia coli. After purification, the proteins were successfully validated using SDS-PAGE and Western blot. BALB/c mice were injected with purified proteins on the first, eighth, and fifteenth days of feeding, respectively; serum samples were collected from mice on the day of immunization and on the 22nd day after immunization. The antibody level in mouse serum was detected by Western blotting using purified expressed proteins as antigens. IL-2, TNF-α and IFN-γ were simultaneously detected in mouse serum by ELISA. The 30 kDa protein was successfully expressed and reacted specifically with the specific serum Mhp His-Tag mouse monoclonal antibody and pig antibody. The expressed recombinant protein was immunogenic. The expression levels of IFN-γ, IL-2 and TNF-α were found to be significantly higher on day 22 than in the control group. This study suggests that the expressed recombinant protein could be used as one of the novel vaccine candidates for Mhp.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2021
• Tom: 24
• Numer: 4
• Opis fizyczny: p.553-561,fig.,ref.

Twórcy

autor

Xu M.

• College of Veterinary Medicine, Hunan Agricultural University, Road 1#, Changsha, 410000, China

autor

Zheng J.

• College of Veterinary Medicine, Hunan Agricultural University, Road 1#, Changsha, 410000, China

autor

Hu S.

• College of Veterinary Medicine, Hunan Agricultural University, Road 1#, Changsha, 410000, China

autor

Wang G.

• College of Veterinary Medicine, Hunan Agricultural University, Road 1#, Changsha, 410000, China

Identyfikatory

DOI

10.24425/pjvs.2021.139980