Three dimensional visualisation and morphometry of bone samples studied in microcomputed tomography (micro-CT)

• Autorzy: Leszczynski B. , Skrzat J. , Kozerska M. , Wrobel A. , Walocha J.
• Języki publikacji: EN

Abstrakty

EN

This article highlights the utility of micro-computed tomography (micro-CT) for characterising microscale bone morphology. For this purpose we tested selected samples of the human bones (Wormian bone, rib, lumbar vertebra) to reconstruct external and internal morphological features. Selected bony samples were investigated using a micro-CT scanner (Skyscan 1172, N.V., Aartselaar, Belgium). The image resolution of scans varied from 5 to 27 µm/pixel depending on the bone sample. We used CTvox software (by Skyscan) to perform volume rendering of the samples. Further, 3-dimensional geometrical models were reconstructed using the CTvol application. Such models enabled graphical distinction between osseous components of various morphology and were used to visualise the Haversian canal system inside the compact bone of the rib. Applying a modified transfer function for volume rendering we presented the overall morphology of the Wormian bone and small vascular channels penetrating its interior. As an example of quantitative analysis based on micro-CT scans we compared the trabecular structure of the lumbar vertebrae with CTAn software. Significant differences in percent bone volume (BV/TV) were determined. Micro-CT was found to be a very accurate and helpful method to study small anatomical structures of the bones in micro scale. (Folia Morphol 2014; 73, 4: 422–428)

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2014
• Tom: 73
• Numer: 4
• Opis fizyczny: p.422-428,fig.,ref.

Twórcy

autor

Leszczynski B.

• Department of Medical Physics, M. Smoluchowski Institute of Physics, Jagiellonian University, Krakow, Poland

autor

Skrzat J.

• Department of Anatomy, Jagiellonian University, Collegium Medicum, Krakow, Poland

autor

Kozerska M.

• Department of Anatomy, Jagiellonian University, Collegium Medicum, Krakow, Poland

autor

Wrobel A.

• Department of Medical Physics, M. Smoluchowski Institute of Physics, Jagiellonian University, Krakow, Poland

autor

Walocha J.

• Department of Anatomy, Collegium Medicum, Jagiellonian University, Krakow, Poland

Bibliografia

• 1. Bouxsein ML, Boyd SK, Christiansen BA, Guldberg RE, Jepsen KJ, Müller R (2010) Guidelines for assessment of bone microstructure in rodents using micro-computed tomography. J Bone Miner Res, 25: 1468–1486.
• 2. Durand EP, Rüegsegger P (1992) High-contrast resolution of CT images for bone structure analysis. Med Phys, 19: 569–573.
• 3. Fajardo RJ, Ryan TM, Kappelman J (2002) Assessing the accuracy of high-resolution X-ray computed tomography of primate trabecular bone by comparison with histological sections. Am J Phys Anthropol, 118: 1–10.
• 4. Feldkamp LA, Davis LC, Kress JW (1984) Practical conebeam algorithm. J Opt Soc Am A, 1: 612–619.
• 5. Flohr TG, Raupach R, Bruder H (2009) Cardiac CT: How much can temporal resolution, spatial resolution, and volume coverage be improved? JCCT, 3: 143–152.
• 6. Genant HK, Jiang Y (2006) Advanced imaging assessment of bone quality. Ann N Y Acad Sci, 1068:410–428.
• 7. Hahn M, Vogel M, Pompesius-Kempa M, Delling G (1992) Trabecular bone pattern factor: a new parameter for simple quantification of bone microarchitecture. Bone, 13: 327–330.
• 8. Klink T, Nagel H, Schwartz B, Regier M, Adam G, Begemann PG (2012) 256-MSCT image acquisition with sequential axial scans: evaluation of image quality and resolution in a phantom study. Rofo, 184: 248–255.
• 9. Matsubara K, Koshida H, Sakuta K, Takata T, Horii J, Iida H, Koshida K, Ichikawa K, Matsui O (2012) Radiation dose and physical image quality in 128-section dual-source computed tomographic coronary angiography: a phantom study. J Appl Clin Med Phys, 13: 3959.
• 10. Poznyakovskiy AA, Zahnert T, Kalaidzidis Y, Schmidt R, Fischer B, Baumgart J, Yarin YM (2008) The creation of geometric three-dimensional models of the inner ear based on micro computer tomography data. Hearing Research 243: 95–104.
• 11. Rüegsegger P, Koller B, Müller R (1996) A microtomographic system for the nondestructive evaluation of bone architecture. Calcif Tissue Int, 58: 24–29.
• 12. Scherf H, Tilgner R (2009) A new high-resolution computed tomography (CT) segmentation method for trabecular bone architectural analysis. Am J Phys Anthropol, 14: 39–51.
• 13. Stauber M, Müller R (2005) Volumetric spatial decomposition of trabecular bone into rods and plates: a new method for local bone morphometry. Bone, 38: 475–484.
• 14. Scanco (2013) XtremeCT Scanco Medical. Available from: http://www.scanco.ch/fileadmin/webmaster_img/Brochures/XtremeCT_v3.pdf.
• 15. Skyscan (2013) CTvox Quick Start Guide (for Software Version 2.6). Availbale from: http://www.skyscan.be/next/CTvox_QuickStartGuide.pdf.
• 16. Skyscan (2013) Structural parameters measured by the SkyscanTM CT-analyser software. Availbale from: http://www.skyscan.be/next/ctan_ctvol_02.pdf.

Identyfikatory

DOI

10.5603/FM.2014.0064