Sodium nitroprusside, a nitric oxide donor, fails to bypass the block of neuronal differentiation in PC12 cells imposed by a dominant negative Ras protein

• Autorzy: Bator J. , Varga J. , Berta G. , Barbakadze T. , Mikeladze D. , Ramsden J. , Szeberenyi J.
• Języki publikacji: EN

Abstrakty

EN

Nitric oxide (NO) is a mediator of a diverse array of inter- and intracellular signal transduction processes. The aim of the present study was to analyze its possible role as a second messenger in the process of neuronal differentiation of PC12 pheochromocytoma cells. Upon NGF treatment wildtype PC12 cells stop dividing and develop neurites. In contrast, a PC12 subclone (designated M-M17-26) expressing a dominant-negative mutant Ras protein keeps proliferating and fails to grow neurites after NGF treatment. Sodium nitroprusside (SNP), an NO donor, was found to induce the p53 protein and to inhibit proliferation of both PC12 and M-M17-26 cells, but failed to induce neuronal differentiation in these cell lines. Key signaling pathways (the ERK and Akt pathways) were also not affected by SNP treatment, and the phosphorylation of CREB transcription factor was only slightly stimulated. It is thus concluded from the results presented in this paper that NO is unable to activate signaling proteins acting downstream or independent of Ras that are required for neuronal differentiation.

Słowa kluczowe

EN

sodium nitroprusside; nitric oxide; bypass; block; neuronal differentiation; PC12 cell; Ras protein; nerve growth factor; p53 protein; ERK protein; Akt protein; CREB protein; nitric oxide synthase; signal transduction

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2012
• Tom: 17
• Numer: 3
• Opis fizyczny: p.323-332,fig.,ref.

Twórcy

autor

Bator J.

• Department of Medical Biology, Medical School, University of Pecs, 7624 Pecs, Szigeti u.12, Hungary

autor

Varga J.

autor

Berta G.

autor

Barbakadze T.

autor

Mikeladze D.

autor

Ramsden J.

autor

Szeberenyi J.

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