Antagonistic effects of Bacillus cereus strain B-02 on morphology, ultrastructure and cytophysiology of Botrytis cinerea

• Autorzy: Li F.-X. , Ma H.-Q. , Liu J. , Zhang C.
• Języki publikacji: EN

Abstrakty

EN

The study on antagonistic mechanism of biocontrol strains gives the premise and basis for efficient and stable biological control. This study aimes to overcome of biocontrol agent in aspects of complicated and diversified mode of action, short-lasting and unstable efficacy in the production processes. This study elucidated the antagonistic mechanism of Bacillus cereus strain B-02 on Botrytis cinerea by detecting changes in morphology, ultrastructure and physiology in affected hyphae of Botrytis cinerea. Which provided certain theoretical and practical significance for biological control of gray mould caused by B. cinerea. B. cereus strain B-02 isolated from tomato rhizosphere mightily suppressed gray mold in tomato caused by B. cinerea. Spore germination and hyphal growth of B. cinerea were inhibited by B. cereus strain B-02. Changes of cell morphology such as distortion, shrinking and swelling were observed by SEM. TEM observation further indicated the ultrastructural alterations of hyphae, including mitochondrion reduction, un-membranous inclusion in cytoplasm, considerable thickening of cell walls, and electronic density enhancement. LSCM observation revealed the fluorescence intensity of nucleus DNA, mitochondrion DNA and reactive oxygen radical in treated hyphae were all stronger than control and the difference was significant (P < 0.01). These results indicated that the antagonistic effects of B. cereus strain B-02 on B. cinerea were likely due to a combination of abnormal synthesis of nucleus DNA and mitochondrion DNA and multifarious ultrastructural alterations in hyphal cell.

Słowa kluczowe

EN

antagonistic effect; Bacillus cereus; B-02 strain; morphology; ultrastructure; cytophysiology; Botrytis cinerea; Botrytis cereus; gray mould; greenhouse; tomato; chemical control

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2012
• Tom: 61
• Numer: 2
• Opis fizyczny: p.119-128,fig.,ref.

Twórcy

autor

Li F.-X.

• School of Life Sciences, Shan Dong University of Technology, Zibo 255049, China

autor

Ma H.-Q.

autor

Liu J.

autor

Zhang C.

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