Effects of lead sulfate on genetic and epigenetic changes, and endogenous hormone evels in corn (Zea mays L.)

• Autorzy: Erturk F.A. , Agar G. , Arslan E. , Nardemir G. , Aydin M. , Taspinar M. S.
• Języki publikacji: EN

Abstrakty

EN

In this research we aimed to evaluate DNA damage levels, DNA methylation, and protein and pytohormone level changes in corn seedlings (Zea mays L.) exposed to 5, 10, 20, and 40 mM concentrations of lead sulfate solution (PbSO4). The results showed that all concentrations of the test material caused a decreasing mitotic index, genomic template stability (GTS), and soluble protein levels, but increased RAPDs profile changes (DNA damage) and DNA hypermethylation. Moreover, in the HPLC (high-pressure liquid chromatography) analyses, it was also observed that Pb contamination caused a decrease in the growth-promoting hormones including gibberellic acid (GA), zeatin (ZA), and indole acetic acid (IAA) levels, in contrast to increasing the absisic acid (ABA) level. The results of this experiment have clearly shown that Pb has a significant impact on the epigenetic mechanisms as well as its genotoxic effects. Some of phytohormone decreases (GA, ZA and IAA) and especially increasing ABA levels under Pb stress may be a part of the defense system against stress.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2014
• Tom: 23
• Numer: 6
• Opis fizyczny: p.1925-1932,fig.,ref.

Twórcy

autor

Erturk F.A.

• Department of Molecular Biology, Faculty of Science, Avrasya University, Trabzon, Turkey

autor

Agar G.

• Department of Biology, Faculty of Science, Ataturk University, Erzurum 25240, Turkey

autor

Arslan E.

• Department of Biology, Faculty of Science, Ataturk University, Erzurum 25240, Turkey

autor

Nardemir G.

• Department of Molecular Biology and Genetics, Faculty of Science, Agri Ibrahim Cecen University, Agri, Turkey

autor

Aydin M.

• Department of Field Crops, Faculty of Agriculture, Ataturk University, Erzurum, Turkey

autor

Taspinar M. S.

• Department of Agricultural Biotechnology, Faculty of Agriculture, Ataturk University, Erzurum, Turkey

Bibliografia

• 1. HERNANDEZ-ALLiCA J., GARBiSU C., BARRUTiA O., BECERRiL J.M. EDTA-induced heavy metal accumulation and phytotoxicity in cardoon plants. Environ. Exp. Bot., 60, 26, 2007.
• 2. SHARMA P., DUBEY R.S. Modulation of nitrate reductase activity in rice seedlings under aluminium toxicity and water stress: role of osmolytes as enzyme protectant. J. Plant Physiol., 162, 854, 2005.
• 3. CENKCi S., CiGERCi i.H., YILDIZ M., OZAY C., BOZ- DAG A., TERZi H. Lead contamination reduces chlorophyll biosynthesis and genomic template stability in Brassica rapa L. Environ. Exp. Bot., 67, 467, 2010.
• 4. ATiCi O., AGAR G., BATTAL P. Changes in phytohormone contents in chickpea seeds germinating under lead or zinc stress. Biol. Plantarum, 49, 215, 2005.
• 5. EKMEKCi Y., TANYOLAC D., AYHAN B. A crop tolerat­ing oxidative stress induced by excess lead: maize. Acta Physiol. Plant., 31, 319, 2009.
• 6. SAMARDAKiEWiCZ S., WOZNY, A. Cell division in Lemna minor roots treated with lead. Aquat. Bot., 83, 289, 2005.
• 7. GE X.C., ZONG H., ZHAN S.X., CHEN J.C., SUN C.R., CAO, K.M. Cloning and expression analysis of a human putative tumor suppressor gene homologue from rice. Acta Bot. Sin., 44, 562, 2002.
• 8. LU Y., RONG T., CAO M. Analysis of DNA methylation in different maize tissues. Journal of Genetics and Genomics, 35, 41, 2008.
• 9. GiCHNER T., ZNiDAR I., SZAKOVA J. Evaluation of DNA damage and mutagenicity induced by lead in tobacco plants. Mutat. Res., 652, 186, 2008.
• 10. MARTiENSSEN R.A., RICHARDS E.J. DNA methylation in eukaryotes. Curr. Opin. Genet. Dev., 5, 234, 1995.
• 11. Si Y., ZHANG C., MENG S., DANE, F. Gene expression changes in response to drought stress in Citrullus colocyn- this. Plant Cell Rep., 28, 997, 2009.
• 12. ZHOU J., WANG X., JiAO Y., QiN Y., LiU X., HE K., CHEN C., MA L., WANG J., XiONG L., ZHANG Q., FAN L., DENG X.W. Global genome expression analysis of rice in response to drought and high-salinity in shoot, flag leaf, and panicle. Plant Mol. Biol., 63, 591, 2007.
• 13. Li X.L., LiN Z.X., NiE Y.C., GUO X.P., ZHANG X.L. Methylation sensitive amplification polymorphism of epige- netics changes in cotton under salt stress. Acta Agronomica Sinica, 35, 588, 2009.
• 14. ZHAO X., CHAl Y., LiU B. Epigenetic inheritance and variation of DNA methylation level and pattern in maize intra-specific hybrids. Plant Sci., 172, 930, 2007.
• 15. WANG C., WANG Z., LUO J., Li Q., Li Y., AHN K., PROWS D.R., WU R. A model for transgenerational imprint­ing variation in complex traits. Plos One, 5, e11396, 2010.
• 16. ZHANG X., YAZAKi J., SUNDARESAN A., COKUS S., CHAN S.W.L., CHEN H., HENDERSON I.R., SHiNN P., PELLEGRiNi M., JACOBSEN S.E., ECKER J.R. Genome-wide High-Resolution-Mapping and Functional Analysis of DNA Methylation in Arabidopsis. Cell, 126, 1189, 2006.
• 17. RUiZ-GARCiA L., CERVERA E.M.T., MARTiNEZ-ZAP- ATER J.M. DNA methylation increases throughout Arabidopsis development. Planta, 222, 301, 2005.
• 18. AKiMOTO K., KATAKAMi H., KiM H.J., OGAWA E., SANO C.M., WADA Y., SANO H. Epigenetic inheritance in rice plants. Ann. Bot-London, 100, 205, 2007.
• 19. POSPtSiLOVA J. Participation of phytohormones in the stomatal regulation of gas exchange during water stress. Biol. Plantarum, 46, 491, 2003.
• 20. ATiCi O., AGAR G. Interaction between endogenous plant hormones and a amylase in germinating chichpea seeds under cadmium exposure. Fresen. Environ. Bull., 12, 781, 2003.
• 21. TURKER M., BATTAL P., AGAR G., GULLUCE M., SAHiN F., EREZ M.E., YiLDiRiM N. Allelopathic effects of plants extracts on physiological and cytological process­es during maize seed germination. Allelopathy Journal, 21, 273, 2008.
• 22. SHULTZ R.W., SETTLAGE S.B., HANLEY-BOWDOIN L. THOMPSON W.F. A trichloroacetic acid-acetone method greatly reduces infrared autofluorescence of protein extracts from plant tissue. Plant Mol. Biol. Rep., 23, 405, 2005.
• 23. ERTURK F.A., AGAR G., ARSLAN E., NARDEMIR G., SAHIN Z. Determination of genomic instability and DNA methylation effects of Cr on maize (Zea mays L.) using RAPD and CRED-RA analysis. Acta Physiologiae Plantarum, 36, 1529, 2014.
• 24. LAEMMLi U.K. Cleavage of structural proteins during assembly of head of Bacteriophage-T4. Nature, 227, 680, 1970.
• 25. KURAiSHi S., TASAKi K., SAKURAi N., SADATOKU K. Changes in levels of cytokinins in etiolated squash seedlings after illumination. Plant Cell Physiol., 32, 585, 1991.
• 26. FUJiOKA S., SAKURAi A., YAMAGUCHi I., MURO- FUSHi N., TAKAHASHi N., KAiHARA S., TAKiMOTO A., CLELAND C.F. Flowering and endogenous levels of plant hormones in Lemna species. Plant Cell Physiol., 271, 927, 1986.
• 27. CAKMAK I., MARSCHNER H., BANGERT F. Effect of zinc nutritional status on growth, protein metabolism and lev- els of indole-3-acetic acid and other phytohormones in bean (Phaseolus vulgaris L.). J. Exp. Bot. 40, 404, 1989.
• 28. UNYAYAR S., ÇELiK A., ÇEKiÇ F.O., GOZEL A. Cadmium-induced genotoxicity, cytotoxicity and lipid per- oxidation in Allium sativum and Vicia faba. Mutagenesis, 21, 77, 2006.
• 29. Li G., QUiROS C.F. Sequence-Related Amplified Polymorphism (SRAP), a new marker system based on a simple PCR reaction: Its application to mapping and gene tagging in Brassica. Theor. Appl. Genet., 103, 455, 2001.
• 30. SAVVA D. Use of DNA fingerprinting to detect genotoxic effects. Ecotox. Environ. Safe., 41, 103, 1998.
• 31. ATiENZAR F.A., JHA A.N. The random amplified poly­morphic DNA (RAPD) assay and related techniques applied to genotoxicity and carcinogenesis studies: A critical review. Mutat. Res.-Rev. Mutat., 613, 76, 2006.
• 32. ATiENZAR F.A., CORDi B., DONKiN M.E. Comparison of ultraviolet-induced genotoxicity detected by random amplified polymorphic DNA with chlorophyll fluorescence and growth in a marine macroalgae, Palnaria palnata. Aquat. Toxicol., 50, 1, 2000.
• 33. TASPiNAR M.S., AGAR G., YiLDlRiM N., SUNAR S., AKSAKAL O., BOZARi S. Evaluation of selenium effect on cadmium genotoxicity in Vicia faba using RAPD. Journal of Food Agriculture and Environment, 7, 857, 2009.
• 34. TASPiNAR M.S., AGAR G., ALPSOY L., YiLDiRiM N., BOZARi S. The protective role of Zinc and Calcium in Vicia faba seedlings subjected to cadmium stres. Toxicol. Ind. Health., 27, 73, 2011.
• 35. SUNAR S., AKSAKAL O., YiLDiRiM N., AGAR G., GULLUCE M., SAHiN F. Genetic diversity and relation­ships detected by FAME and RAPD analysis among Thymus species growing in Eastern Anatolia region of Turkey. Romanian Biotechnological Letters, 144, 267, 2009.
• 36. BASHA M.R., WEi W., BAKHEET S.A., BENiTEZ N., SiDDiQi H.K., GE Y.W., LAHiRi D.K., ZAWiA N.H. The fetal basis of amyloidogenesis: Exposure to lead and latent overexpression of amyloid precursor protein and beta-amy­loid in the aging brain. J. Neurosci., 25, 823, 2005.
• 37. OZFiDAN C., TURKAN I., SEKMEN A.H., SECKiN B. Time course analysis of ABA and non-ionic osmotic stress- induced changes in water status, chlorophyll flourescens and osmotic adjustment in Arabidopsis thaliana wild-type (Colombia) and ABA deficient mutant (aba2). Environ. Exp. Bot., 86, 44, 2013.
• 38. CHiNNUSAMY V., GONG Z., ZHU J.K. Abscisic acid- mediated epigenetic processes in plant development and stress responses. Journal of Integrative Plant Biology, 50, 1187, 2008.
• 39. BRADFORD M.M. A rapid and sensitive method for the quan- titation of microgram quantities of protein utilizing the princi­ple of protein-dye binding. Anal. Biochem., 72, 248, 1976.

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