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2012 | 56 | 4 |

Tytuł artykułu

Occurrence and expression of toxin genes in Clostridium perfringens isolates from pigs

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Clostridium perfringens isolates were obtained from pigs of five porcine farms in Poland. The presence of C. perfringens was detected in 92% of faeces samples and its number ranged from 1.0 x 10¹ cfu/g to 1.2 x 10⁷ cfu/g. All the isolates belonged to type A and 48.7% of them contained cpb2 gene. The qualitative assessment of toxin genes expression by type A subtype ß2 isolates showed expression of cpa gene in 100% of strains and cpb2 gene in 71% of the analysed strains. The isolate from one-day-old piglets demonstrated also the expression of cpa and cpb2 genes.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

56

Numer

4

Opis fizyczny

p.495-498,fig.,ref.

Twórcy

autor
  • Department of Hygiene of Animal Feeding Stuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland
  • Department of Hygiene of Animal Feeding Stuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Hygiene of Animal Feeding Stuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Hygiene of Animal Feeding Stuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

  • 1. Baums C.G., Schotte U., Amtsberg G., Goethe R.: Diagnostic multiplex PCR for toxin genotyping of Clostridium perfringens isolates. Vet Microbiol 2004, 100, 11-16.
  • 2. Bueschel D.M., Jost B.H., Billington S.J., Trinh H.T., Songer J.G.: Prevalence of cpb2, encoding beta2 toxin, in Clostridium perfringens field isolates: correlation of genotype with phenotype. Vet Microbiol 2003, 94, 121- 129.
  • 3. Crespo R., Fisher D.J., Shivaprasad H.L., Fernandez- Miyakawa M.E., Uzal F.A.: Toxinoypes of Clostridium perfringens isolated from sick and healthy avian species. J Vet Diagn Invest 2007, 19, 329-333.
  • 4. Garmory, H.S., Chanter, N., French, N.P., Bueschel, D., Songer, J.G., Titball, R.W., Occurrence of Clostridium perfringens beta2-toxin amongst animals, determined using genotyping and subtyping PCR assays. Epidemiol Infect 2000, 124, 61-67.
  • 5. Gibert M., Jolivet-Renaud C., Popoff M.R.: Beta2 toxin, a novel toxin produced by Clostridium perfringens. Gene 1997, 203, 65-73.
  • 6. Grave K., Jensen V.F., Odensvik K., Wierup M., Bangen M.: Usage of veterinary therapeutic antimicrobials in Denmark, Norway and Sweden following termination of antimicrobial growth promoter use. Prev Vet Med 2006, 75, 123-132.
  • 7. Herholz C., Miserez R., Nicolet J., Frey J., Popoff M., Gibert M., Gerber H., Straub R.: Prevalence of ß₂ - toxinogenic Clostridium perfringens in horses with intestinal disorders. J Clin Microbiol 1999, 37, 358-361.
  • 8. Johansson A., Aspan A., Bagge E., Baverud V., Engström B.E., Johansson K.E.: Genetic diversity of Clostridium perfringens type A isolates from animals, food poisoning outbreaks and sludge. BMC Microbiol 2006, 6, 1-34.
  • 9. Keyburn A.L., Boyce J.D., Vaz P., Trudi L., Bannam T.L., Mark E., Ford M.E., Parker D., Rubbo A.D., Rood J.I., Moore R.J. NetB, a new toxin that is associated with avian necrotic enteritis caused by Clostridium perfringens. PLOS Pathogens 2008, 4, 1-11.
  • 10. Keyburn A.L., Trudi L., Bannam T.L., Immerseel F.V., Rood J.I., Moore R.J.: Association between avian necrotic enteritis and Clostridium perfringens strains expressing NetB toxin. Vet Res 2010, 41, 1-8.
  • 11. PN-EN 13401:2000 "Microbiology of food and animal feeding stuffs. Horizontal method for enumeration of Clostridium perfringens".
  • 12. PN-R-64791:1994 "Feeding stuffs - Requirements and microbiological examination".
  • 13. Sawires Y.S., Songer J.G.: Clostridium perfringens: Insight into virulence evolution and population structure. Anaerobe 2006, 12, 23-43.
  • 14. Sipos W., Fischer L., Schindler M., Schmoll F.: Genotyping of Clostridium perfringens isolated from domestic and exotic ruminants and swine. J Vet Med B Infect Dis Vet Public Health 2003, 50, 360-362.
  • 15. Songer J.G.: Clostridial enteric diseases of domestic animals. Clin Microbiol Rev 1996, 9, 216-234.
  • 16. Songer J.G., Miskimmins D.W.: Clostridium perfringens type E enteritis in calves: two cases and brief review of the literature. Anaerobe 2004, 10, 239-242.
  • 17. Wasiński B., Pejsak Z.: Evaluation of phenotypic and genotypie features of Clostridium perfringens strains isolated from suckling piglets. Medycyna Wet 2008, 64, 791-795.
  • 18. Waters M., Savoie A., Garmory H.S., Bueschel D., Popoff M.R., Songer J.G., Titball R.W., McClane B.A., Sarker M.R.: Genotyping and phenotyping of beta2- toxigenic Clostridium perfringens fecal isolates associated with gastrointestinal diseases in piglets. J Clin Microbiol 2003, 41, 3584-3591.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-fb05704e-c886-41a6-8194-83b28b9ed507
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