The influence of three endodontic sealers on bone healing: an experimental study

• Autorzy: Chisnoiu R. , Moldovan M. , Pastrav O. , Delean A. , Chisnoiu A.M.
• Języki publikacji: EN

Abstrakty

EN

Background: The aim of this experimental study is to assess the bone healing phenomenon produced in the presence of 3 dental materials used for the root canal obturation. Materials and methods: The biocompatibility of 3 endodontic sealers (a self-curing epoxy resin — AH Plus, a dual cure urethane dimethacrylate resin — RealSeal and a new dual cure endodontic hydroxyapatite based filling material) was investigated after intra-osseous implantation of the materials in rats’ calvaria. Tissue reaction was studied at 2, 4, 6, 8, 10 and 12 weeks after implantation using calibrated image retrieval by Olympus. We took into consideration the presence of inflammatory cells (polymorphonuclear leukocytes, macrophages, plasma cells, lymphocytes and giant cells) and classified the aspects of the histological samples according to the following scale: 0 — no inflammation, 1 — mild, isolated inflammation, 2 — moderate, localised inflammatory reaction, 3 — severe, diffuse and intense inflammatory reaction. Results: The inflammatory reaction was present at the 6 intervals for all the tested materials, but a decrease of the inflammatory infiltrate, statistically significant, until extinction for all tested materials was observed at the end of the experimental period. The reaction of bone tissue recovery was most intense in the case of the control area. Lower intensity osteogenesis phenomenon was observed in case of all 3 tested sealers at the end of the experimental period. Conclusions: Biocompatibility and bone healing induction of the new hydroxyapatite based endodontic filling material is comparable to other commercial materials, AH Plus and RealSeal. (Folia Morphol 2016; 75, 1: 14–20)

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2016
• Tom: 75
• Numer: 1
• Opis fizyczny: p.14-20,fig.,ref.

Twórcy

autor

Chisnoiu R.

• Department of Odontology, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania

autor

Moldovan M.

• “Raluca Ripan” Institute for Research in Chemistry, “Babes Bolyai” University, Cluj-Napoca, Romania

autor

Pastrav O.

• Department of Odontology, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania

autor

Delean A.

• Department of Odontology, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania

autor

Chisnoiu A.M.

• Department of Prosthodontics, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania

Bibliografia

• 1. Batista RF, Hidalgo MM, Hernandes L, Consolaro A, Velloso TR, Cuman RK (2007) Microscopic analysis of subcutaneous reactions to endodontic sealer implants in rats. J Biomed Mater Res A, 81: 171–177. doi: 10.1002/jbm.a.30918.
• 2. Bouillaguet S, Wataha JC, Tay FR, Brackett MG, Lockwood PE (2006) Initial in vitro biological response to contemporary endodontic sealers. J Endod, 32: 989–992. doi: 10.1016/j.joen.2006.05.006.
• 3. Develioglu H, Unver Saraydin S, Kartal U (2009) The bone-healing effect of a xenograft in a rat calvarial defect model. Dent Mater J, 28: 396–400. doi: 10.4012/dmj.28.396.
• 4. Farhad AR, Hasheminia S, Razavi S, Feizi M (2011) Histopathologic evaluation of subcutaneous tissue response to three endodontic sealers in rats. J Oral Science, 53: 15–21. doi: 10.2334/josnusd.53.15.
• 5. Gopikrishna V, Suresh Chandra B (2014) Grossman’s endodontic practice. 13th ed. LWW.
• 6. Jie Zhao, Yu Liu, Wei-bin Sun, Hai Zhang (2011) Amorphous calcium phosphate and its application in dentistry. Chem Cent J, 5: 35–40. doi: 10.1186/1752-153X-5-40.
• 7. Kikuchi M (2013) Hydroxyapatite/collagen bone-like nanocomposite. Biol Pharm Bull, 36: 1666–1669. doi: 10.1248/bpb.b13-00460.
• 8. Lodiene G, Morisbak E, Bruzell E, Orstavik D (2008) Toxicity evaluation of root canal sealers in vitro. IntEndod J, 41: 72–77. doi: 10.1111/j.1365-2591.2007.01321.x.
• 9. McNamara RP, Henry MA, Schindler WG, Hargreaves KM (2010) Biocompatibility of accelerated mineral trioxide aggregate in a rat model, J Endod, 36: 1851–1855. doi: 10.1016/j.joen.2010.08.021.
• 10. Moretton TR, Brown CE, Legan JJ, Kafrawy AH (2000) Tissue reactions after subcutaneous and intraosseous implantation of mineral trioxide aggregate and ethoxybenzoic acid cement. J Biomed Mater Res, 52: 528–533. doi: 10.1002/1097-4636(20001205)52:3<528::AID-JBM11>3.0.CO;2-9.
• 11. Olsson B, Sliwkowski A, Langeland K (1981) Subcutaneous implantation for the biological evaluation of endodontic materials. J Endod, 7: 355–336. doi: 10.1016/S0099-2399(81)80003-9.
• 12. Onay EO, Ungor M, Ozdemir BH (2007) In vivo evaluation of the biocompatibility of a new resin-based obturation system, Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 104: e60–66. doi: 10.1016/j.tripleo.2007.03.006.
• 13. Porto GG, Vasconcelos BC, Andrade ES, Carneiro SC, Frota MS (2012) Is a 5 mm rat calvarium defect really critical? Acta Cir Bras, 27: 757–760. doi: 10.1590/S0102-86502012001100003.
• 14. Schmalz G., Arenholt-Bindslev D (2009) Biocompatibility of dental materials. Springer-Verlag, Berlin Heidelberg.
• 15. Sousa CJ, Montes CR, Pascon EA, Loyola AM, Versiani MA (2006) Comparison of the intraosseous biocompatibility of AH Plus, EndoREZ, and Epiphany root canal sealers. J Endod, 32: 656–662. doi: 10.1016/j.joen.2005.12.003.
• 16. Tienen TG, Heijkants RG, de Groot JH, Schouten AJ, Pennings AJ, Veth RP, Buma P (2006) Meniscal replacement in dogs. Tissue regeneration in two different materials with similar properties. J Biomed Mater Res B Appl Biomater, 76: 389–396. doi: 10.1002/jbm.b.30406.
• 17. Wataha JC, Rueggeberg FA, Lapp CA (1999) In vitro cytotoxicity of resin-containing restorative materials after aging in artificial saliva. Clin Oral Investigat, 3: 144–149. doi: 10.1007/s007840050093.

Identyfikatory

DOI

10.5603/FM.a2015.0079