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2018 | 67 | 4 |
Tytuł artykułu

Comparison of the photosensitivity of biofilms of different genera of cariogenic bacteria in tooth slices

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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
This study compared the outcome of photosensitization on the viability of four different cariogens in planktonic form as well as biofilms in human dentine. Photodynamic therapy was carried out with a gallium aluminium arsenide laser (670 nm wavelength) using Toluidine blue O (TBO) as the photosensitizer. Cariogenic bacteria (Streptococcus mutans, Lactobacillus casei, Streptococcus salivarius and Actinomyces viscosus) were exposed to TBO and then to the laser for 1 minute in planktonic suspension. Then, tooth slices previously incubated for 24 hours with broth cultures of broth culture of the four cariogenic organisms were exposed to antimicrobial photosensitization. The control samples consisted of planktonic and sessile cells that were exposed to TBO alone, laser alone and the bacterial cells that were not treated with TBO or laser. The results showed significant reductions in the viability of S. mutans, L.casei and A. viscosus in both planktonic form (to 13%, 30%, and 55%, respectively) and sessile form hosted in dentinal tubules (to 19%, 13% and 52%, respectively), relative to the controls. S.salivarius was the least affected in planktonic (94% viability) and sessile form (86% viability). In conclusion, sensitivity to photosensitization is species-dependent and sessile biofilm cells are affected to the same extent as their planktonic counterparts.
Wydawca
-
Rocznik
Tom
67
Numer
4
Opis fizyczny
p.455-461,fig.,ref.
Twórcy
autor
  • Department of Applied Biology, Faculty of Faculty of Science and Arts, Jordan University of Science and Technology, Irbid, Jordan
  • Department of Biology, College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia
  • Department of Restorative Dentistry, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan
  • Department of Physics, Faculty of Science and Arts, Jordan University of Science and Technology, Irbid, Jordan
Bibliografia
  • Aas JA, Griffen AL, Dardis SR, Lee AM, Olsen I, Dewhirst FE, Leys EJ, Paster BJ. 2008. Bacteria of dental caries in primary and permanent teeth in children and young adults. J Clin Microbiol. 46:1407–1417.
  • Burns T, Wilson M, Pearson GJ. 1994. Killing of cariogenic bacteria by light from a gallium aluminium arsenide diode laser. J Dent. 22:273–278.
  • Burns T, Wilson M, Pearson GJ. 1995. Effect of dentine and collagen on the lethal photosensitization of Streptococcus mutans. Caries Res. 29:192–197.
  • Chan Y, Lai CH. 2003. Bactericidal effects of different laser wavelengths on periodontopathic germs in photodynamic therapy. Lasers Med Sci. 18:51–55.
  • Chávez De Paz LE, Hamilton IR, Svensäter G. 2008. Oral bacteria in biofilms exhibit slow reactivation from nutrient deprivation. Microbiology. 154:1927–1938.
  • Chen YY, Clancy KA, Burne RA. 1996. Streptococcus salivarius urease: genetic and biochemical characterization and expression in a dental plaque streptococcus. Infect Immun. 64:585–592.
  • Chen YY, Weaver CA, Burne RA. 2000. Dual functions of Streptococcus salivarius urease. J Bacteriol. 182:4667–4669.
  • Decker EM, Klein C, Schwindt D, Von Ohle C. 2014. Metabolic activity of Streptococcus mutans biofilms and gene expression during exposure to xylitol and sucrose. Int J Oral Sci. 6:195–204.
  • Do T, Damé-Teixeira N, Naginyte M, Marsh PD. 2017. Root surface biofilms and caries. Monogr Oral Sci. 26:26–34.
  • Edwardsson S. 1987. Bacteriology of dentine caries. In: Thylstrup A, Leach SA, Qvist V, editors. Dentine and dentine reactions in the oral cavity. Oxford (UK): IRL Press. p. 95–102.
  • Giusti JS, Santos-Pinto L, Pizzolito AC, Helmerson K, Carvalho-Filho E, Kurachi C, Bagnato VS. 2008. Antimicrobial photodynamic action on dentine using a light-emitting diode light source. Photomed Laser Surg. 26:281–287.
  • Gross EL, Beall CJ, Kutsch SR, Firestone ND, Leys EJ, Griffen AL. 2012. Beyond Streptococcus mutans: dental caries onset linked to multiple species by 16S rRNA community analysis. PLoS ONE 7:e47722.
  • Jakubovics NS, Gill SR, Iobst SE, Vickerman MM, Kolenbrander PE. 2008. Regulation of gene expression in a mixed-genus community: stabilized arginine biosynthesis in Streptococcus gordonii by coaggregation with Actinomyces naeslundii. J Bacteriol. 190:3646–3657.
  • Jakubovics NS, Kolenbrander PE. 2010. The road to ruin: the formation of disease-associated oral biofilms. Oral Dis. 16: 729–739.
  • Krzyściak W, Kościelniak D, Papież M, Vyhouskaya P, Zagórska-Świeży K, Kołodziej I, Bystrowska B, Jurczak A. 2017. Effect of a Lactobacillus salivarius probiotic on a double-species Streptococcus mutans and Candida albicans caries biofilm. Nutrients. 9(11):1242.
  • Lee YH, Park HW, Lee JH, Seo HW, Lee SY. 2012. The photodynamic therapy on Streptococcus mutans biofilms using erythrosine and dental halogen curing unit. Int J Oral Sci. 4:196–201.
  • Love RM, McMillan MD, Park Y, Jenkinson HF. 2000. Coinvasion of dentinal tubules by Porphyromonas gingivalis and Streptococcus gordonii depends upon binding specificity of streptococcal antigen I/II adhesin. Infect Immun. 68:1359–1365.
  • Mang TS, Tayal DP, Baier R. 2012. Photodynamic therapy as an alternative treatment for disinfection of bacteria in oral biofilms. Lasers Surg Med. 44:588–596.
  • Marsh PD. 2004. Dental plaque as a microbial biofilm. Caries Res. 38:204–211.
  • Melo MAS, Rolim JPML, Passos VF, Lima RA, Zanin ICJ, Codes MB, Rocha SS, Rodrigues LKA. 2015. Photodynamic antimicrobial chemotherapy and ultra-conservative caries removal linked for management of deep caries lesions. Photodiagnosis Photodyn Ther. 12:581–586.
  • Metcalf D, Robinson C, Devine D, Wood S. 2006. Enhancement of erythrosine-mediated photodynamic therapy of Streptococcus mutans biofilms by light fractionation. J Antimicrob Chemother. 58:190–192.
  • Munson MA, Banerjee A, Watson TF, Wade WG. 2004. Molecular analysis of the microflora associated with dental caries. J Clin Microbiol. 42:3023–3029.
  • O’Neill F, Hope CK, Wilson M. 2002. Oral bacteria in multi-species biofilms can be killed by red light in the presence of toluidine blue. Lasers Surg Med. 31:86–90.
  • Paulino TP, Ribeiro KF, Thedei GJR, Tedesco AC, Ciancaglini P. 2005. Use of hand held photopolymerizer to photoinactivate Streptococcus mutans. Arch Oral Biol. 50:353–359.
  • Ricatto LGO, Conrado LAL, Turssi CP, França FMG, Basting RT, Amaral FLB. 2014. Comparative evaluation of photodynamic therapy using LASER or light emitting diode on cariogenic bacteria: An in vitro study. Eur J Dent. 8:509–514.
  • Ruby JD, Li Y, Luo Y, Caufield PW. 2002. Genetic characterization of the oral actinomyces. Arch of Oral Biol. 47:457–463.
  • Samaranayake LP. 2012. Essential microbiology for dentistry. 4th ed. London (UK): Churchill Livingstone.
  • Schoop U, Kluger W, Moritz A, Nedjelik N, Georgopoulos A, Sperr W. 2004. Bactericidal effect of different laser systems in the deep layers of dentine. Lasers Surg Med. 35:111–116.
  • Seal DJ, Ng YL, Spratt D, Bhatti M, Gulabivala K. 2002. An in vitro comparison of the bactericidal efficacy of lethal photosensitization or sodium hyphochlorite irrigation on Streptococcus intermedius biofilms in root canals. Int Endod J. 35:268–274.
  • Smith DJ. 2002. Dental caries vaccines: prospects and concerns. Crit Rev Oral Biol Med. 13:335–349.
  • Socransky SS, Haffajee AD. 1997. The nature of periodontal diseases. Ann Periodontol. 2:3–10.
  • Tanzer JM, Livingston J, Thompson AM. 2001. The microbiology of primary dental caries in humans. J Dent Educ. 65:1028–1037.
  • Ten Cate JM. 2013. Contemporary perspective on the use of fluoride products in caries prevention. Br Dent J. 214:161–167.
  • Tonon CC, Paschoal MA, Correia M, Spolidório DM, Bagnato VS, Giusti JS, Santos-Pinto L. 2015. Comparative effects of photodynamic therapy mediated by curcumin on standard and clinical isolate of Streptococcus mutans. J Contemp Dent Pract. 16:1–6.
  • Van Acker H, Coenye T. 2016. The role of efflux and physiological adaptation in biofilm tolerance and resistance. J Biol Chem. 291:12565–12572.
  • van Ruyven FOJ, Lingstrom P, van Houte J, Kent R. 2000. Relationship among mutans streptococci, “low-pH” bacteria, and iodophilic polysaccharide-producing bacteria in dental plaque and early enamel caries in humans. J Dent Res. 79:778–784.
  • Welin-Neilands J, Svensater G. 2007. Acid tolerance of biofilm cells of Streptococcus mutans. Appl Environ Microbiol. 73: 5633–5638.
  • Whittaker CJ, Klier CM, Kolenbrander PE. 1996. Mechanisms of adhesion by oral bacteria. Annu Rev Microbiol. 50:513–552.
  • Williams AJ, Pearson GJ, Colles MJ, Wilson M. 2004. The photoactivated antibacterial action of toluidine blue O in a collagen matrix and in carious dentine. Caries Res. 38:530–536.
  • Williams J A, Pearson G J, Colles M J, Wilson M. 2003. The effect of variable energy input from a novel light source on the photoactivated bactericidal action of toluidine blue O on Streptococcus mutans. Caries Res. 37:190–193.
  • Wilson M, Burns T, Pratten J, Pearson GJ. 1995. Bacteria in supragingival plaque samples can be killed by low-power laser light in the presence of a photosensitizer. J Appl Bacteriol. 78:569–574.
  • Zanin IC, Lobo MM, Rodrigues LK, Pimenta LA, Hofling JF, Goncalves RB. 2006. Photosensitization of in vitro biofilms by toluidine blue O combined with a light-emitting diode. Eur J Oral Sci. 114:64–69.
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Bibliografia
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