Effect of growth regulators and other components of culture medium on morphogenesis of sugar beet (Beta vulgaris L.) in unfertilised ovule in vitro cultures

• Autorzy: Tomaszewska-Sowa M.

Warianty tytułu

PL

Wpływ regulatorów wzrostu i innych składników pożywki na morfogenezę buraka cukrowego (Beta vulgaris L.) w kulturach in vitro niezapłodnionych zalążków

• Języki publikacji: EN

Abstrakty

EN

The aim of the present research was to optimise the process of plant regeneration from unfertilised ovules in sugar beet (Beta vulgaris L.). A two-stage method was applied which involved preculture in liquid MS medium and culture on solid media. The study evaluated the effectiveness of different groups of growth regulators, agar and gelrite on the morphogenesis of explants. To do so, the culture was maintained on 21 MS media containing auxins (NAA, IBA, 2,4-D), cytokinins (BAP, KIN, TDZ), antiauxin (TIBA) as well as solidifying substances at different concentrations and combinations. Varied effectiveness of callus and shoot formation was observed depending on the medium used. The highest efficiency of shoot formation was recorded on the media containing 4.4 μM BAP, 0.09 M sucrose, and 0.04% gelrite. The formation of roots was induced when exposed to 14.8 μM IBA and 0.049 μM 2iP, and the percentage of rooted shoots ranged from 0.0 to 65.2%, depending on the growth regulator combination used for shoot regeneration.

PL

Celem przeprowadzonych badań była optymalizacja procesu regeneracji roślin z niezapłodnionych zalążków buraka cukrowego (Beta vulgaris L.). Zastosowano metodę dwufazową obejmującą prekulturę w płynnej pożywce MS i kulturę na pożywkach stałych. Oceniono skuteczność oddziaływania różnych grup regulatorów wzrostu oraz agaru i gerlitu na morfogenezę eksplantatów. W tym celu kulturę prowadzono na 21 pożywkach MS zawierających auksyny (NAA, IBA, 2,4-D), cytokininy (BAP, KIN, TDZ), antyauksynę (TIBA) a także substancje zestalające w różnych stężeniach i kombinacjach. Zaobserwowano zróżnicowaną efektywność formowania kalusa i pędów w zależności od zastosowanego podłoża. Najwyższą wydajność formowania pędów uzyskano na pożywkach zawierających 4,4 μM BAP, 0,09 M sacharozy i 0,04% gerlitu. Formowanie korzeni indukowano w obecności 14,8 μM IBA i 0,049 μM 2iP, a udział ukorzenionych pędów wahał się od 0,0 do 65,2%, w zależności od wariantu doświadczenia.

• Wydawca: -
• Czasopismo: Acta Agrobotanica
• Rocznik: 2012
• Tom: 65
• Numer: 4
• Opis fizyczny: p.91-100,fig.,ref.

Twórcy

autor

Tomaszewska-Sowa M.

• Department of Plant Physiology and Fundamentals of Biotechnology, University of Technology and Life Sciences in Bydgoszcz, Bernardynska 6, 85-029 Bydgoszcz, Poland

Bibliografia

• Bo s s o u t r o t D. , Ho s ema n s D. 1985. Gynogenesis in Beta vulgaris L.: from in vitro culture of unpollinated ovules to the production of doubled haploid plants in soil. Plant Cell Rep. 4: 300–303. http://dx.doi.org/10.1007%2FBF00269883
• D’Ha l l u i n K. , Ke ime r B. 1986. Production of haploid sugar beets (Beta vulgaris L.) by ovule culture. [In:] Genetic Manipulation in Plant Breeding, W. Horn, C.J. Jerser, W. Odenbach, O.Schieder, (eds). Walter de Gruyter Berlin, New York. 307–309.
• Do c t r i n a l M ., S a ngwa n R.S., S a ngwa n-No r - r e e l B . S . 1989. In vitro gynogenesis in Beta vulgaris L.: Effects of plant growth regulators, temperature, genotypes and season. Plant Cell Tiss. Org. Cult. 17: 1–12. http://dx.doi.org/10.1007%2F978-3-642-61499-6_17
• Do c t r i n a l M . , S a ngwa n R .S, S a ngwa n-No r r e e l B. S . 1990. Sugarbeet (Beta vulgaris L.): In vitro induction of haploids. Biotechnol. Agric. For. Haploids in Crop Improvement, 12: 346–357.
• Do v z h e n k o A . , K o o p H .U. 2003. Sugarbeet (Beta vulgaris L.): shoot regeneration from callus and protoplasts. Planta, 217: 374–381. http://dx.doi.org/10.1007%2Fs00425-003-1006-7
• F e r r a n t V. , Bo u h a r mo n t J . 1994. Origin of gynogenetic embryos of Beta vulgaris L. Sexual Plant Rep. 7: 12–16.
• Font e s M.A., O t on i W.C ., C a r ol i no S.M.B. , Brommons c h e n k el S . H ., Font e s E .P.B. , F a r i M , L u o r o R . P. 1999. Hyperhydricity in pepper plants regeneration in vitro: involvement of BiP (Binding Protein) and ultrastructural aspects. Plant Cell Rep. 19: 81–87. http://dx.doi.org/10.1007%2Fs002990050714
• Gamb o r g O. L . , M i l l e r R .A. , O j iwa K . 1968. Nutrient requirements of suspension culture of soybean root callus. Exp. Cell Res. 50: 151–158.
• Goś k a M. 1997. Haploidy i podwojone haploidy buraka cukrowego (Beta vulgaris L.) oraz możliwości ich wykorzystania w hodowli. / Haploids and doubled haploids of sugar beet (Beta vulgaris L.) and their use in breeding. Monografie i rozprawy naukowe. IHAR, 2: 1–81. (in Polish)
• Goś k a M. 2004. Wpływ cytokinin na morfogenezę buraka cukrowego (Beta vulgaris L.) w kulturach in vitro. / Effect of cytokinins on morphogenesis of sugar beet (Beta vulgaris L.) in in vitro cultures. Biotechnologia, 2 (65): 137–145. (in Polish).
• Goś k a M. , J a s s em B. 1988. Histological observations of sugar-beet ovules in in vitro cultures. Bull. Pol. Acad. Sci. Biol. Sci. 36: 171–175.
• Gü r e l S . , G ü r e l E . , K a y a Z . 2000. Doubled haploid plant production from unpollinated ovules of sugar beet (Beta vulgaris L.). Plant Cell Rep. 19: 1155–1159.
• Ha ns en A., G e r t z A., Jo e r sbo M., A nde r s e n S.B. 1998. Antimicrotubule herbicides for in vitro chromosome doubling in Beta vulgaris L. ovule culture. Euphytica, 101: 231–237.
• Ha n s e n A .L., G e r t z A ., Jo e r sb o M., A n d e r s e n S . B. 2000. Chromosome doubling in vitro with mamiprophos methyl in Beta vulgaris ovule culture. Acta Agric. Scand. 50: 89–95.
• Ho s ema n s D. , Bo s s o u t r o t D. 1983. Induction of haploid plants from in vitro culture of unpollinated beet ovules (Beta vulgaris L.). Z. Pflanzenzchtg. 91: 74–77.
• J o y c e S . , C a s s e l l s A .C . , J a i n S .M. 2003. Stress and aberrant phenotypes in in vitro culture. Plant Cell, Tiss. Org. Cult. 74: 103–121.
• Kuykend a l l L .D. , S au nd e r s J .W. , S t o c k e t t T.M. 2003. Rhizobium radiobacter conjugation and callus independent shoot regeneration used to introduce the cercosporin export gene cfp from cercospora into sugar beet (Beta vulgaris L.). Biotechnol. Lett. 25: 739–744.
• L u x H . , H e r r ma n n L . , We t z e l C . 1990. Production of haploid sugar beet (Beta vulgaris L.) by culturing unpollinated ovules. Plant Breed. 104: 177–183. http://dx.doi.org/10.1111%2Fj.1439-0523.1990.tb00420.x
• Me z e i S . , K o v a c e v L . , N a g l N . 2006. Sugar beet micropropagation. Biotechnol. & Biotechnol. Eq. 20: 9–14.
• Mu r a s h i g e T. , S k o o g F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15: 473–497.
• Piq u e r a s A . , C o r t i na M . , S e r na M . D. , C a s a s J .L. 2002. Polyamines and hyperhydricity in micropropagated carnation plants. Plant Sci. 162: 671–678. http://dx.doi.org/10.1016%2FS0168-9452%2802%2900007-9
• R a q u i n C . 1982. Analyse des s gr gations de g nes impliqu s dans la pigmentation florale des p tunias issus d’androgen se / Analysis of genes segregation involved in floral pigmentation in petunias from androgenesis. C. R. Acad. Sc. Paris, 294: 335–338.
• S c h e n k R .U., H i ld e b r a n d t A .C . 1972. Medium and techniques for the induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50: 199–204. http://dx.doi.org/10.1139%2Fb72-026
• S t e f a n i a k B. 2004. Roślinne kultury in vitro. Komórki roślinne w warunkach stresu. / Plant in vitro cultures. Plant cells under stress conditions. In: A. Woźny, K. Przybyło (eds.). UAM Poznań, Poland: 14–91. (in Polish).
• Te t u T. , S a ngwa n R .S. , S a ngwa n-No r r e e l B .S. 1987. Hormonal control of organogenesis and somatic embryogenesis in Beta vulgaris callus. J. Exp. Bot. 38: 506–517.
• Va n G ey t J , Sp o ckma n n J r. G . J. , H a l lu i n K .D. , J a c o b s M. 1987. In vitro induction of haploid plants from unpollinated ovules and ovaries of the sugarbeet (Beta vulgaris L.). Theor. Appl. Genet. 73: 920–925.
• Wiś n i ews k a E . 2006. Charakterystyka cytologiczna i genetyczna komórek o wysokim i niskim potencjale do regeneracji roślin in vitro (Nicotiana tabacum L. Vs Beta vulgaris L.). / Cytological and genetic characteristics of cells with high and low potential for in vitro regeneration of plants (Nicotiana tabacum L. Vs. Beta vulgaris L.). IHAR. PhD Thesis, 1–149. (in Polish).
• Yu M.H. 1992. Growth and reproduction performance of ovule-induced sugarbeet plants. Sabrao J. 24: 47–55.
• Zh a ng C .L. , C h e n D.F. , E l l i o t M .C ., S l a t e r A . 2001. Thidiazuron-induced organogenesis and somatic embryogenesis in sugar beet (Beta vulgaris L.). In Vitro Cell. Dev. Biol. Plant. 37: 305–310. http://dx.doi.org/10.1007%2Fs11627-001-0054-6
• Z h u Z .C . , Wu H . 1979. In vitro induction of haploid plants from the unpollinated ovaries of Triticum aestivum and Nicotiana tabacum. Acta Genet. Sin. 6: 181–183.

Uwagi

Rekord w opracowaniu