Overexpression of heat shock protein gene PfHSP21.4 in Arabidopsis thaliana enhances heat tolerance

• Autorzy: Zhang L. , Zhang Q. , Gao Y. , Pan H. , Shi S. , Wang Y.
• Języki publikacji: EN

Abstrakty

EN

Nuclear-encoded chloroplast small heat shock proteins (Cp-sHSPs) play important roles in plant stress tolerance due to their abundance and diversity. Their functions in Primula under heat treatment are poorly characterized. Here, expression analysis showed that the Primula Cp-sHSP gene, PfHSP21.4, was highly induced by heat stress in all vegetative and generative tissues in addition to constitutive expression in certain development stages. PfHSP21.4 was introduced into Arabidopsis, and its function was analysed in transgenic plants. Under heat stress, the PfHSP21.4 transgenic plants showed increased heat tolerance as shown by preservation of hypocotyl elongation, membrane integrity, chlorophyll content and photosystem II activity (Fv/Fm), increased seedling survival and increase in proline content. Alleviation of oxidative damage was associated with increased activity of superoxide dismutase and peroxidase. In addition, the induced expression of HSP101, HSP70, ascorbate peroxidase and Δ1-pyrroline-5-carboxylate synthase under heat stress was more pronounced in transgenic plants than in wild-type plants. These results support the positive role of PfHSP21.4 in response to heat stress in plants.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 06
• Opis fizyczny: p.1555-1564,fig.,ref.

Twórcy

autor

Zhang L.

• Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, China National Engineering Research Centre for Floriculture School of Landscape Architecture, Beijing Forestry University, 100083 Beijing, China

autor

Zhang Q.

• Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, China National Engineering Research Centre for Floriculture School of Landscape Architecture, Beijing Forestry University, 100083 Beijing, China

autor

Gao Y.

• Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, China National Engineering Research Centre for Floriculture School of Landscape Architecture, Beijing Forestry University, 100083 Beijing, China

autor

Pan H.

• Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, China National Engineering Research Centre for Floriculture School of Landscape Architecture, Beijing Forestry University, 100083 Beijing, China

autor

Shi S.

• Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, China National Engineering Research Centre for Floriculture School of Landscape Architecture, Beijing Forestry University, 100083 Beijing, China

autor

Wang Y.

• Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, China National Engineering Research Centre for Floriculture School of Landscape Architecture, Beijing Forestry University, 100083 Beijing, China

Bibliografia

• Barua D, Downs CA, Heckathorn SA (2003) Variation in chloroplast small heat-shock protein function is a major determinant of variation in thermotolerance of photosynthetic electron transport among ecotypes of Chenopodium album. Funct Plant Biol 30:1071–1079
• Beyer WF, Fridovich Y (1987) Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal Biochem 161:559–566
• Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
• Charng YY, Liu HC, Liu NY, Chi WT, Wang CN, Chang SH, Wang TT (2007) A heat-inducible transcription factor, HsfA2, is required for extension of acquired thermotolerance in Arabidopsis. Plant Physiol 143:251–262
• Chauhan H, Khurana N, Nijhavan A, Khurana JP, Khurana P (2012) The wheat chloroplastic small heat shock protein (sHSP26) is involved in seed maturation and germination and imparts tolerance to heat stress. Plant Cell Environ 35:1912–1931
• Chen Q, Vierling E (1991) Analysis of conserved domains identifies a unique structural feature of a chloroplast heat shock protein. Mol Gen Genet 226:425–431
• Downs CA, Jones LR, Heckathorn SA (1999a) Evidence for a novel set of small heat shock proteins that associates with the mitochondria of murine PC12 cells and protects NADH: ubiquinone oxidoreductase from heat and oxidative stress. Arch Biochem Biophys 365:344–350
• Downs CA, Ryan SL, Heckathorn SA (1999b) The chloroplast small heat shock protein: evidence for a general role in protecting photosystem II against oxidative stress and photoinhibition. J Plant Physiol 155:488–496
• Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930
• Guo SJ, Zhou HY, Zhang XS, Li XG, Meng QW (2007) Overexpression of CaHSP26 in transgenic tobacco alleviates photoinhibition of PSII and PSI during chilling stress under low irradiance. J Plant Physiol 164:126–136
• Haq NU, Raza S, Luthe DS, Heckathorn SA, Shakeel S (2013) A dual role for the chloroplast small heat shock protein of Chenopodium album including protection from both heat and metal stress. Plant Mol Biol Rep 31:398–408
• Heckathorn SA, Downs CA, Sharkey TD, Coleman JS (1998) The small, methionine-rich chloroplast heat-shock protein protects photosystem II electron transport during heat stress. Plant Physiol 116:439–444
• Heckathorn S, Ryan SL, Baylis JA, Wang D, Hamilton EW, Cundiff L, Luthe DS (2002) In vivo evidence from an Agrostis stolonifera selection genotype that chloroplast small heat-shock proteins can protect photo-system II during heat stress. Funct Plant Biol 29:933–944
• Jiang CH (2009) Cloning and functional analysis of RcHSP17.8 gene encoding a small heat shock protein in Rosa Chinensis. Dissertation, University of Fudan
• Jiang C, Xu J, Zhang H, Zhang X, Shi J, Li M, Ming F (2009) A cytosolic class I small heat shock protein, RcHSP17. 8, of Rosa chinensis confers resistance to a variety of stresses to Escherichia coli, yeast and Arabidopsis thaliana. Plant Cell Environ 32:1046–1059
• Kim KH, Alam I, Kim YG, Sharmin SA, Lee KW, Lee SH, Lee BH (2012) Overexpression of a chloroplast-localized small heat shock protein OsHSP26 confers enhanced tolerance against oxidative and heat stresses in tall fescue. Biotechnol Lett 34:371–377
• Lea PJ, Blackwell RD (1993) Photosynthesis and production in a changing environment: a field and laboratory manual. Chapman and Hall, UK, pp 313–336
• Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Meth Enzymol 148:350–382
• MacAdam JW, Nelson CJ, Sharp RE (1992) Peroxidase activity in the leaf elongation zone of tall fescue. Plant Physiol 99:872–878
• Marangoni AG, Palma T, Stanley DW (1996) Membrane effects in postharvest physiology. Postharvest Biol Technol 7:193–217
• Maxwell K, Johnson GN (2000) Chlorophyll fluorescence–a practical guide. J Exp Bot 345:659–668
• Osteryoung KW, Vierling E (1994) Dynamics of small heat shock protein distribution within the chloroplasts of higher plants. J Biol Chem 269:28676–28682
• Pang CH, Li K, Wang BS (2011) Overexpression of SsCHLAPXs confers protection against oxidative stress induced by high light in transgenic Arabidopsis thaliana. Physiol Plant 143:355–366
• Preczewski P, Heckathorn SA, Downs CA, Coleman JS (2000) Photosynthetic thermotolerance is positively and quantitatively correlated with production of specific heat-shock proteins among nine genotypes of tomato. Photosynthetica 38:127–134
• Quan RD, Shang M, Zhang H, Zhao YX, Zhang JR (2004) Improved chilling tolerance by transformation with betA gene for the enhancement of glycinebetaine synthesis in maize. Plant Sci 166:141–149
• Sanmiya K, Suzuki K, Egawa Y, Shono M (2004) Mitochondrial small heat-shock protein enhances thermotolerance in tobacco plants. FEBS Lett 557:265–268
• Sato Y, Yokoya S (2008) Enhanced tolerance to drought stress in transgenic rice plants overexpressing a small heat-shock protein, sHSP17.7. Plant Cell Rep 27:329–334
• Scarpeci TE, Zanor MI, Valle EM (2008) Investigating the role of plant heat shock proteins during oxidative stress. Plant Signal Behav 3:856–857
• Shakeel S, Haq NU, Heckathorn SA, Hamilton EW, Luthe DS (2011) Ecotypic variation in chloroplast small heat-shock proteins and related thermotolerance in Chenopodium album. Plant Physiol Biochem 49:898–908
• Shakeel SN, Haq NU, Heckathorn S, Luthe DS (2012) Analysis of gene sequences indicates that quantity not quality of chloroplast small HSPs improves thermotolerance in C4 and CAM plants. Plant Cell Rep 31:1943–1957
• Sharom M, Willemot C, Thompson JE (1994) Chilling injury induces lipid phase changes in membranes of tomato fruit. Plant Physiol 105:305–308
• Stewart GR, Robertson BD, Young DB (2004) Analysis of the function of mycobacterial DnaJ proteins by overexpression and microarray profiling. Tuberculosis 84:180–187
• Tarantino D, Vianelli A, Carraro L, Soave C (1999) A nuclear mutant of Arabidopsis thaliana selected for enhanced sensitivity to light-chill stress is altered in PSII electron transport activity. Plant Physiol 107:361–371
• Torok Z, Goloubinoff P, Horvath I, Tsvetkova NM, Glatz A, Balogh G, Varvasovszki V, Los DA, Vierling E, Crowe JH, Vigh L (2001) Synechocystis HSP17 is an amphitropic protein that stabilizes heat-stressed membranes and binds denatured proteins for subsequent chaperone-mediated refolding. Proc Natl Acad Sci USA 98:3098–3103
• Ukaji N, Kuwabara C, Takezawa D, Arakawa K, Yoshida S, Fujikawa S (1999) Accumulation of small heat-shock protein homologs in the endoplasmic reticulum of cortical parenchyma cells in mulberry in association with seasonal cold acclimation. Plant Physiol 120:481–490
• Valcu CM, Lalanne C, Plomion C, Schlink K (2008) Heat induced changes in protein expression profiles of Norway spruce (Picea abies) ecotypes from different elevations. Proteomics 8(20): 4287–4302
• Volkov RA, Panchuk II, Mullineaux PM, Schoffl F (2006) Heat stress-induced H₂O₂ is required for effective expression of heat shock genes in Arabidopsis. Plant Mol Biol 61:733–746
• Wang D, Luthe DS (2003) Heat sensitivity in a bentgrass variant. Failure to accumulate a chloroplast heat shock protein isoform implicated in heat tolerance. Plant Physiol 133:319–327
• Wang WX, Vinocur B, Shoseyov O, Altman A (2004a) Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. Trends Plant Sci 9:244–252
• Wang YH, Ying Y, Chen J, Wang XC (2004b) Transgenic Arabidopsis overexpressing Mn-SOD enhanced salt-tolerance. Plant Sci 167:671–677
• Wang Y, Wisniewski M, Meilan R, Cui M, Fuchigami L (2006) Transgenic tomato (Lycopersicon esculentum) overexpressing cAPX exhibits enhanced tolerance to UV-B and heat stress. J Appl Hortic 8:87–90
• Wang Y, Ye Q, Zhang M, Yang C (2012) Involvement of Arabidopsis CPR5 in thermotolerance. Acta Physiol Plant 34:2093–2103
• Waters ER, Rioflorido I (2007) Evolutionary analysis of the small heat shock proteins in five complete algal genomes. J Mol Evol 5:162–174
• Waters ER, Vierling E (1999) Chloroplast small heat shock proteins: evidence for atypical evolution of an organelle-localized protein. Proc Natl Acad Sci 96:14394–14399
• Waters ER, Aevermann BD, Sanders-Reed Z (2008) Comparative analysis of the small heat shock proteins in three angiosperm genomes identifies new subfamilies and reveals diverse evolutionary patterns. Cell Stress Chaperones 13:127–142
• Xue Y, Peng R, Xiong A, Li X, Zha D, Yao Q (2010) Overexpression of heat shock protein gene hsp26 in Arabidopsis thaliana enhances heat tolerance. Biol Plant 54:105–111
• Zhang Y, Mian MAR, Chekhovskiy K, So S, Kupfer D, Lai H, Roe BA (2005) Differential gene expression in Festuca under heat stress conditions. J Exp Bot 56:897–907
• Zhou Y, Chen H, Chu P, Li Y, Tan B, Ding Y, Huang S (2012) NnHSP17.5, a cytosolic class II small heat shock protein gene from Nelumbo nucifera, contributes to seed germination vigor and seedling thermotolerance in transgenic Arabidopsis. Plant Cell Rep 31:379–389
• Zoran R, Urska B, Prasad PV (2007) Correlation between heat stability of thylakoid membranes and loss of chlorophyll in winter wheat under heat stress. Crop Sci 47:2067–2073

Identyfikatory

DOI

10.1007/s11738-014-1531-y