Identyfikacja translokacji 1BL/1RS i substytucji 1B/1R za pomocą markerów STS-PCR oraz prążków C w pszenicy

• Autorzy: Kowalczyk K. , Chrzastek M. , Miazga D.

Warianty tytułu

EN

Identification of 1BL/1RS translocation and 1B/1R substitution using STS-PCR markers and C-banding in wheat

• Języki publikacji: PL

Abstrakty

PL

W pracy identyfikowano translokację 1BL/1RS w polskich odmianach pszenicy oraz substytucję 1B/1R w linii 'Grana'/'Dańkowskie Złote', stosując markery cytologiczne i molekularne. Analizując rozmieszczenie prążków heterochromatynowych w chromosomach, zidentyfikowano substytucję 1B/1R w linii 'Grana'/'Dańkowskie Złote' oraz translokację 1BL/1RS w odmianach 'Aleta', 'Jubilatka', 'Lama', 'Lanca', 'Olma', 'Weneda' i "Wilga'. Wyniki te potwierdzono przy pomocy metody STS-PCR. Zastosowano dwa startery J07IF1 (5'-TAAGCC- GTAAAGCATGGTGCAC-3') i J07IR1 (5 '-CTTCAACGAATGTTTTCCTC- TTC-3') specyficzne dla krótkiego ramienia chromosomu 1R. Po zakończeniu reakcji i rozdzieleniu za pomocą elektroforezy stwierdzono obecność prążka o wielkości 1200 par zasad (pz) tylko w odmianach: 'Aleta', 'Jubilatka', 'Lama', 'Lanca', 'Olma', 'Weneda' i 'Wilga' oraz w linii substytucyjnej 'Grana'/'Dańkowskie Złote' a także w życie odmiany 'Dańkowskie Złote'.

EN

In the paper the translocation 1BL/1RS in Polish wheat cultivars and substitution 1B/1R in 'Grana'/'Dańkowskie Złote' line were identificated using cytological and molecular markers. On the basis C-banding method the substitution 1B/1R in 'Grana'/'Dańkowskie Złote' line and the translocation 1BL/1RS in 'Aleta', 'Jubilatka', 'Lama', 'Lanca', 'Olma', 'Weneda' and 'Wilga' cultivars were identificated. These results were confirmed using the STS-PCR method. Two primers J07IF1 (5'-TAAGCCGTAAAGCATGGTGCAC-3') and J07IR1 (5'- CTTCAACGAATGTTTTCCTCTTC-3'), specific to short arm of 1R chromosome were used. After the reaction and the electrophoretic separation, the presence of a fragment of the size 1200 bp was found only in the cultivars 'Aleta', 'Jubilatka', 'Lama', 'Lanca', 'Olma', "Weneda' and 'Wilga' as well as in 'Grana'/'Dańkowskie Złote' substitution line and in 'Dańkowskie Złote' rye.

• Wydawca: -
• Czasopismo: Zeszyty Problemowe Postępów Nauk Rolniczych
• Rocznik: 2002
• Tom: 488
• Numer: 1
• Opis fizyczny: s.169-177,fot.,tab.,bibliogr.

Twórcy

autor

Kowalczyk K.

• Instytut Genetyki i Hodowli Roślin, Akademia Rolnicza, ul.Akademicka 15, 20-394 Lublin

autor

Chrzastek M.

• Instytut Genetyki i Hodowli Roślin, Akademia Rolnicza, ul.Akademicka 15, 20-394 Lublin

autor

Miazga D.

• Instytut Genetyki i Hodowli Roślin, Akademia Rolnicza, ul.Akademicka 15, 20-394 Lublin

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