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2011 | 51 | 3 |

Tytuł artykułu

Pectobacterium carotovorum ssp. carotovorum groźnym patogenem cukinii

Warianty tytułu

EN
Pectobacterium carotovorum ssp. carotovorum – a serious zucchini pathogen

Języki publikacji

PL

Abstrakty

EN
The aim of the presented studies was to determine a casual agent of zucchini soft rot under the field conditions in the region of Kujawy. In 2009 and 2010, after heavy rain and high temperatures, a mass occurrence of soft rot on the zucchini stems was recorded, leading to a significant reduction in the yield. From zucchini diseased tissue with the soft rot symptoms, some cultures of bacteria were isolated. The isolates giving a positive reaction in pathogenicity test were marked as C1, C2 and C3. Phenotypic characterization of the C1 and C3 isolates allowed to classify them into Erwinia genus and the isolate C2 into Corynebacterium. The further identification using BIOLOG system and sequencing 16S rDNA partial genes, allowed finally to identicate the isolates C1 and C3 as Pectobacterium carotovorum ssp. carotovorum and isolate C2 as Corynebacterium. Studied isolates caused strong soft rot on cucumber and zucchini slices in less than 24 hours after inoculation. The same isolates were reisolated from rotting tissue what confirmed their identity with those ones used for inoculation.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

51

Numer

3

Opis fizyczny

s.1150-1154,tab.,bibliogr.

Twórcy

Bibliografia

  • Bhat N.A., Bhat S.D., Masoodi S.A., Mir M.Y., Zargar P., Sheikh A. 2010. Studies on status and host range of soft rot disease of cabbage (Brassica oleracea var. capitata) in Kashmir Valley J. Phytology 2 (10): 55–59. www.journal-phytology.com: 2075–6240.
  • Edwards U., Rogall T., Blöcker H., Emde M., Böttger E.C. 1989. Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res. 17: 7843–7853.
  • Cupples D., Kelman A. 1974. Evaluation of selective media for isolation of soft-rot bacteria from soil and plant tissue. Phytopathology 64: 468–475.
  • Gracia-Garza J.A., Blom T.J., Brown W., Allen W. 2002. Pre- and post-plant applications of copper-based compounds to control Erwinia soft rot of calla lilies. Can. J. Plant Pathol. 24: 274–280.
  • Kotoujansky A. 1987. Molecular genetics of pathogenesis by soft rot Erwinia. Ann. Rev. Phytopathol. 25: 405–430.
  • Mikiciński A., Sobiczewski P., Berczyński S. 2010. Nowe możliwości biologicznej i chemicznej ochrony roślin przed zarazą ogniową (Erwinia amylovora). Prog. Plant Protection/Post. Ochr. Roślin 50 (3): 1347–1351.
  • Pérombelon M.C.M., Salmond G.P.C. 1995. Bacterial soft rots. p. 1–20. In: „Pathogenesis and Host Specificity in Plant Diseases” Vol. 3 (R.P. Singh, ed.). Elsevier Science, Oxford, 417 pp.
  • Schaad N.W., Jones J.B., Chun W. 2001. Laboratory Guide for Identification of Plant Pathogenic Bacteria. 3rd ed. The APS, St. Paul, MN, 371 pp.
  • Snijder R.C., Van Tuyl J.M. 2002. Evaluation of test of determine resistance of Zantedeschia spp. (Araceae) to soft rot caused by Erwinia carotovora subsp. carotovora. Eur. J. Plant Pathol. 108: 565–571.
  • Wright P.J., Burge G.K., Triggs C.M. 2002. Effects of cessation of irrigation and time of lifting of tubers on bacterial soft rot of calla (Zantedeschia spp.) tubers. N.Z. J. Crop Hort. Sci. 30: 265–272.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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