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2012 | 34 | 2 |

Tytuł artykułu

Pulvinus: an ideal explant for plant regeneration in Caesalpinia bonduc (L.) Roxb., an important ethnomedicinal woody climber

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
This study demonstrates the morphogenic potential of pulvinus, an important organ situated at the base of the petiole or rachis of leguminous plants. Plant regeneration via pulvinus-derived calli of Caesalpinia bonduc has been achieved. Organogenic calli have been derived from the explant 45 days after culture on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) alone or in combination with 6-benzylaminopurine (BA). Optimum callus induction (100%) occurred when the pulvini were cultured on MS medium fortified with 6 mg l⁻¹ 2,4-D and 1 mg l⁻¹ BA. The highest shoot induction was obtained when the calli were transferred to MS medium supplemented with 5 mg l⁻¹ BA and 1 mg l⁻¹ indole-3-acetic acid (IAA). On this medium, 87% cultures responded with an average number of 4.2 shoots per culture. The maximum root induction from the regenerated shoots was observed on half strength MS medium containing 6 mg l⁻¹ indole-3-butyric acid (IBA). Here 100% shoots rooted with a mean number of 6.3 roots per shoot. The regenerated plantlets were acclimatized and subsequently showed normal growth. This efficient protocol will be helpful for propagating elite clones on a mass scale and could be utilized for genetic transformation study.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

34

Numer

2

Opis fizyczny

p.693-699,fig.,ref.

Twórcy

  • Postgraduate and Research Department of Botany, St. Tomas College, Pala, Arunapuram (P.O.), Kottayam 686 574, Kerala, India
autor
  • Postgraduate and Research Department of Botany, St. Tomas College, Pala, Arunapuram (P.O.), Kottayam 686 574, Kerala, India
  • The Rapinat Herbarium and Centre for Molecular Systematics and Taxonomy, St. Joseph's College (Autonomous), Tiruchirappalli 620 002, Tamil Nadu, India
autor
  • Postgraduate and Research Department of Botany, St. Tomas College, Pala, Arunapuram (P.O.), Kottayam 686 574, Kerala, India

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Bibliografia

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