Induction of resistance in tomato plants against Fusarium oxysporum f. sp. lycopersici mediated through salicylic acid and Trichoderma harzianum

• Autorzy: Ojha S. , Chatterjee N. C.
• Języki publikacji: EN

Abstrakty

EN

The effect of a soil application of salicylic acid (SA) and a biocontrol agent, Trichoderma harzianum (TH) on the induction of phenolic accumulation content and defense enzymes in tomato plants infected with Fusarium oxysporum f. sp. lycopersici (F. oxysporum) was investigated. The phenolic content was recorded to be higher in all the treatments viz. F. oxysporum, F. oxysporum + TH, F. oxysporum + SA and F. oxysporum + TH + SA than that of the healthy plants and reached its maximum level in the plants treated with F. oxysporum + TH + SA. Tomato plants treated with different concentrations of SA (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mM) showed significant increases in the activities of both peroxidase and polyphenol oxidase where a prominent enhancement was observed at a 1.5 mM concentration of SA. F. oxysporum infection resulted in the induction of both of the enzyme activities but at a lower level. The activities of both peroxidase and polyphenol oxidase reached their maximum on the 28th day, when the plants were treated with F. oxysporum and SA (1.5 mM). The combined application of SA (1.5 mM) and TH in Fusarium infected tomato plants, also enhanced the activities of both of the enzymes.

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2012
• Tom: 52
• Numer: 2
• Opis fizyczny: p.220-225,ref.

Twórcy

autor

Ojha S.

• Department of Botany, The University of Burdwan Burdwan - 713104, West Bengal, India

autor

Chatterjee N. C.

Bibliografia

• Abo-Elyousr A. M. K., Hussein M. A. M., Allam A. D. A., Hassan A. H. M. 2008. Enhanced onion resistance against stemphylium leaf blight disease, caused by Stemphylium vesicarium, by di-potassium phosphate and benzothiadiazole treatments. Plant Pathol. J. 24 (2): 171-177.
• Benhamou N., Gagne S., Quere D. L., Dehbi L. 2000. Bacterial mediated induced resistance in cucumber beneficial effect of the endophytic bacterium Serratia plymuthica on the protection against infection by Pythium ultimum. Phytopathol. 90 (1): 45-46.
• Bi J. L., Felton G. W. 1995. Foliar oxidative stress and insect herbivory: primary compounds, secondary metabolites and reactive oxygen species as components of induced resistance. J. Chem. Ecol. 21 (9): 1511-1530.
• Cavalcanti F. R., Resende M. L., Lima S. P., Silveira J. A., Oliveira J. T. 2007. Activities of antioxidant enzymes and photosynthetic responses in tomato pre-treated by plant activators and inoculated by Xanthomonas vesicatoria. Physiol. Mol. Plant Pathol. 68 (4-6): 198-208.
• Chakraborty M. R., Chatterjee N. C. 2007. Interaction of Trichoderma harzianum with Furarium solani during its pathogenesis and the associated resistance of the host. Asian J. Exp. Sci. 21 (2): 353-357.
• Chen Z., Lyer S., Caplan A., Klessig D. F., Fan B. 1997. Differential accumulation of salicylic acid-sensitive catalase in different rice tissues. Plant Physiol. 114 (1): 193-201.
• Das S., Aggarwal R., Singh D. V. 2003. Differential induction of defense related enzymes involved in lignin biosynthesis in wheat in response to spot bloth infection. Indian Phytopathol. 56 (2): 129-133.
• Durner J., Shah J., Klessig D. F. 1997. Salicylic acid and disease resistance in plants. Trend. Plant Sci. 2 (7): 266-274.
• Fodor J., Gulner G., Adam A. L., Barna B., Komives T., Kiraly Z. 1997. Local and systemic responses to antioxidants to tobacco mosaic virus infection and to salicylic acid in tobacco. Plant Physiol. 114 (4): 1443-1451.
• Kamalakannan A., Mohan L., Harish S., Radjacommare R., Amutha G., Chitra K., Karuppiah R., Mareeswari P., Rajinimala N., Angayarkanni T. 2004. Biocontrol agents induce disease resistance in Phyllanthus niruri Linn. against damping-off disease caused by Rhizoctonia solani. Phytopathol. Mediterr. 43 (2): 187-194.
• Mahadevan A., Sridhar R. 1982. Methods in Physiological Plant Pathology. Sivakam Publishers, Madras, 316 pp.
• Mehrotra R. S., Aggarwal R. S. 2003. Plant Pathology. Tata McGraw Hill Publishing Company Limited., New Delhi, 846 pp.
• Melo G. A., Shimizu M. M., Mazzafera P. 2006. Polyphenoloxidase activity in coffee leaves and its role in resistance against the coffee leaf miner and coffee leaf rust. Phytochemistry 67 (3): 277-285.
• Morkunas I., Gmerek J. 2007. The possible involvement of peroxidase in defense of yellow lupin embryos axes against Fusarium oxysporum. J. Plant Physiol. 164 (6): 497-506.
• Ojha S., Chakraborty M. R., Chatterjee N. C. 2005. Activities of phenol oxidizing enzymes in anthracnose disease of Saraca asoca under pathogenesis. Indian Biol. 37 (2): 9-11.
• Perello A., Monaco C., Simon M. R., Sisterna M., Dalbello G. 2003. Biocontrol efficacy of Trichoderma isolates for tar spot of wheat in Argentina. Crop Prot. 22 (7): 1099-1106.
• Pradeep T., Jambhale N. D. 2002. Relationship between phenolics, polyphenol oxidase and peroxidase, and resistance to powdery mildew in Zizyphus. Indian Phytopathol. 55 (2): 195-196.
• Ramadoss N. 2002. Studies on Epidemiology, Pathophysiology and Management of Thanjavur Wilt of Coconut. Ph.D. thesis. T. N. Agric. Univ. Coimbatore, 131 pp.
• Sadasivam S., Manickam A. 1996. Biochemical Methods. New Age Int. Pvt. Ltd. Pub. Coimbatore, 256 pp.
• Schonbeck F., Dehne H. W., Beicht W. 1980. Activation of unspecific resistance mechanisms in plants. J. Plant Dis. Prot. 87 (3): 654-666.
• Shimzu N., Hosogi N., Hyon G.S, Jiang S., Inoue K., Park P. 2006. Reactive oxygen species (ROS) generation and ROS induced lipid peroxidation are associated with plant membrane modifications in host cells in response to AK-toxin from Alternaria alternate Japanese pear pathotype. J. Gen. Plant Pathol. 72 (1): 6-15.
• Singleton V. L., Rossi J. A. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Viticul. 16 (3): 144-158.
• Sukand G., Kulkarni S. 2006. Studies on structural and biochemical mechanism of resistance in groundnut to Puccinia arachidis. Indian Phytopathol. 59 (3): 323-328.
• Surplus S. L., Jordan B. R., Murphy A. M., Carr J. P., Thomas B., Mackerness S. A. H. 1998. Ultraviolet induced responses in Arabidopsis thaliana: Role of salicylic acid and reactive oxygen species in the regulation of transcript encoding photosynthetic and acidic pathogenesis-related proteins. Plant Cell Environ. 21 (7): 685-694.
• Tan J., Schneider B., Svatos A., Bendnarek P., Liu J., Hahlbrock K. 2004. Universally occurring phenylpropanoid and specific indolic metabolites in infected and uninfected Arabidopsis thaliana roots and leaves. Phytochemistry 65 (5): 691-699.
• Ye S. F., Zhou H. Y., Sun Y., Zou L. Y., Yu J. Q. 2006. Cinnamic acid causes oxidative stress in cucumber roots and promotes incidence of Fusarium wilt. Environ. Exp. Bot. 56 (3): 255-262.

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