Genes associated with the release of dormant buds in tree peonies (Paeonia suffruticosa)

• Autorzy: Huang X. , Xue T. , Dai S. , Gai S. , Zheng C. , Zheng G.
• Języki publikacji: EN

Abstrakty

EN

A subtractive cDNA library was developed to study genes associated with the release of dormant buds in tree peony. To identify genes that are highly expressed in buds released from dormancy, 588 clones were examined by differential screening and then 185 clones were selected to be sequenced. A total of 31 unique genes were obtained, of which only 25 sequences had matches in the NCBI database or Arabidopsis thaliana protein database while 6 sequences with no matches. Many of the different genes were identified as having unknown or hypothetical functions while others were speculated to have different molecular functions. The expression profiles of the selected ‘‘candidate’’ genes which may be associated with dormancy release according to their putative function and previous reports were assessed by northern blot and semiquantitative RT-PCR. The results indicated that the transcriptional expressions of the isolated genes are related to growth regulation and stress response. Our results provide interesting information for further understanding the molecular mechanism of bud dormancy release in tree peony.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2008
• Tom: 30
• Numer: 6
• Opis fizyczny: p.797-806,fig.,ref.

Twórcy

autor

Huang X.

• College Landscape and Architecture, Beijing Forestry University, 100083 Beijing, People's Republic of China
• College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109 Shandong, People's Republic of China

autor

Xue T.

• State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, 271018 Shandong, People's Republic of China

autor

Dai S.

• College Landscape and Architecture, Beijing Forestry University, 100083 Beijing, People's Republic of China

autor

Gai S.

• College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109 Shandong, People's Republic of China

autor

Zheng C.

• State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, 271018 Shandong, People's Republic of China

autor

Zheng G.

• College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109 Shandong, People's Republic of China

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