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2012 | 53 | 2 |
Tytuł artykułu

Genetic variation of Fusarium oxysporum isolates forming fumonisin B1 and moniliformin

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Thirty single-spore isolates of a toxigenic fungus, Fusarium oxysporum, were isolated from asparagus spears and identified by species-specific polymerase chain reaction (PCR) and translation elongation factor 1-? (TEF) sequence analysis. In the examined sets of F. oxysporum isolates, the DNA sequences of mating type genes (MAT) were identified. The distribution of MAT idiomorph may suggest that MAT1-2 is a predominant mating type in the F. oxysporum population. F. oxysporum is mainly recognised as a producer of moniliformin—the highly toxic secondary metabolite. Moniliformin content was determined by high-performance liquid chromatography (HPLC) analysis in the range 0.05–1,007.47 µg g-1 (mean 115.93 µg g-1) but, also, fumonisin B1 was detected, in the concentration range 0.01–0.91 µg g-1 (mean 0.19 µg g-1). There was no association between mating types and the mycotoxins biosynthesis level. Additionally, a significant intra-species genetic diversity was revealed and molecular markers associated with toxins biosynthesis were identified.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
53
Numer
2
Opis fizyczny
p.237-247,fig.,ref.
Twórcy
  • Department of Phytopathology, Poznan University of Life Sciences, Dabrowskiego 159, 60-594 Poznan, Poland
  • Department of Mathematical and Statistical Methods, Poznan University of Life Sciences, Wojska Polskiego 28, 60-637 Poznan, Poland
  • Department of Chemistry, Poznan University of Life Sciences, Wojska Polskiego 75, 60-625 Poznan, Poland
autor
  • Department of Phytopathology, Poznan University of Life Sciences, Dabrowskiego 159, 60-594 Poznan, Poland
  • Department of Phytopathology, Poznan University of Life Sciences, Dabrowskiego 159, 60-594 Poznan, Poland
autor
  • Department of Chemistry, Poznan University of Life Sciences, Wojska Polskiego 75, 60-625 Poznan, Poland
autor
  • Department of Chemistry, Poznan University of Life Sciences, Wojska Polskiego 75, 60-625 Poznan, Poland
  • Institute of Plant Genetics Polish Academy of Science, Strzeszynska 34, 60-479 Poznan, Poland
Bibliografia
Uwagi
PL
Rekord w opracowaniu
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-f8f1e3c7-3399-4838-bbfa-06377390f57d
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