Use of real-time PCR technique in determination of major fibrolytic and non fibrolytic bacteria present in Indian Surti buffaloes (Bubalus bubalis)

• Autorzy: Singh K.M. , Tripathi A. K. , Pandya P. R. , Parnerkar S. , Rank D. N. , Kothari R. K. , Joshi C. G.
• Języki publikacji: EN

Abstrakty

EN

In the milk industry in India, buffalo breeds are most commonly used for milk production. Efficiency of fiber digestion in ruminants is critical for animal productivity. Bacteria play an important role in fiber digestion and utilization. Absolute quantification real-time PCR was used to quantify ten bacterial species in rumen fluid of Surti buffalo fed green fodder, dry roughage and compound concentrate mixture. Abundance of each target taxon was calculated as a fraction of the total 16S rRNA gene copies in the samples, using taxon-specific primers. Bacterial populations showed a clear predominance of Ruminococcus albus, which comprised 5.66% of the bacterial rRNA gene copies in the samples. However, only 0.9% to 4.24% of the bacterial rRNA gene copies were represented by the ruminal Fibrobacter succinogenes, Ruminococcus flavefaciens and Prevotella species. The proportion of rRNA gene copies attributable to Selenomonas ruminantium, Streptococcus bovis, Ruminobacter amylophilus, Treponema bryantii and Anaerovibrio lipolytica was even less abundant, each comprising <0.11% of the bacterial rRNA gene copies. The data suggest that the aggregate abundance of the most intensively studied ruminal bacterial species is relatively low and that a large fraction of the uncultured population represents a single bacterial genus.

Słowa kluczowe

EN

real-time polymerase chain reaction; determination; fibrolytic bacteria; non-fibrolytic bacteria; Indian Surti buffalo; Bubalus bubalis

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2013
• Tom: 62
• Numer: 2
• Opis fizyczny: p.195-200,fig.,ref.

Twórcy

autor

Singh K.M.

• Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry (CVSAH), Anand Agricultural University (AAU), Anand (388 001), Gujarat, India

autor

Tripathi A. K.

• Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry (CVSAH), Anand Agricultural University (AAU), Anand (388 001), Gujarat, India

autor

Pandya P. R.

• Animal Nutrition Research Station, CVSAH, AAU, Anand, Gujarat, India

autor

Parnerkar S.

• Animal Nutrition Research Station, CVSAH, AAU, Anand, Gujarat, India

autor

Rank D. N.

• Department of Animal Genetics and Breeding, CVSAH, AAU, Anand, Gujarat, India

autor

Kothari R. K.

• Department of Microbiology, Christ College, Rajkot, Gujarat, India

autor

Joshi C. G.

• Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry (CVSAH), Anand Agricultural University (AAU), Anand (388 001), Gujarat, India

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