Impact of operational parameters on bacterial community in a full-scale municipal wastewater treatment plant

• Autorzy: Cydzik-Kwiatkowska A. , Zielinska M. , Wojnowska-Baryla I.
• Języki publikacji: EN

Abstrakty

EN

A bacterial community in activated sludge from a full-scale municipal wastewater treatment plant was monitored throughout the year with the use of FISH, RISA and DGGE techniques. In the investigated range of temperatures (11.9–21.6°C), a rise in temperature resulted in a lower total bacteria richness, while organic load rate changes from 0.09 to 0.21 g COD·g TSS–1 · d–1 were positively correlated with the number of bands in RISA patterns. The most diverse pattern (29 different bands) was characteristic for the activated sludge sample collected at the end of January at wastewater temperature of 11.9°C. The ammonia-oxidising bacteria community did not change during the study, and comprised of 4 different bacterial populations with one dominant species closely related to Nitrosospira sp. REGAU (GenBank accession number AY635572.1). The percentage of ammonia-oxidising bacteria in the activated sludge varied from 6.2 to 19.5% and depended on temperature (R = 0.61, p = 0.02) and organic load rate (R = – 0.55, p = 0.04).

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2012
• Tom: 61
• Numer: 1
• Opis fizyczny: p.41-49,fig.,ref.

Twórcy

autor

Cydzik-Kwiatkowska A.

• Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

autor

Zielinska M.

autor

Wojnowska-Baryla I.

Bibliografia

• Altschul S.F., T.L. Madden, A.A. Scha!er, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman. 1997. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res. 25: 3389–3402.
• Amann R.I., L. Krumholz and D.A. Stahl. 1990. Fluorescentoligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J. Bacteriol. 172: 762–770.
• Ariesyady H.D., T. Ito and S. Okabe. 2007. Functional bacterial and archaeal community structures of major trophic groups in a full-scale anaerobic sludge digester. Water Res. 41: 1554–1568.
• Avrahami S., W. Liesack and R. Conrad. 2003. Effects of temperature and fertilizer on activity and community structure of soil ammonia oxidizers. Environ. Microbiol. 5: 691–705.
• Chae K.J., T. Rameshwar, A. Jang, S.H. Kim and I.S. Kim. 2008. Analysis of the nitrifying bacterial community in BioCube sponge media using fluorescent in situ hybridization (FISH) and microelectrodes. J. Environ. Manage. 88: 1426–1435.
• Cydzik-Kwiatkowska A. and I. Wojnowska-Baryła. 2008. the impact of organic carbon and ammonia load in wastewater on ammonia-oxidizing bacteria community in activated sludge. Pol. J. Microbiol. 57(3): 241–248.
• Daims H., U. Purkhold, L. Bjerrum, E. Arnold, P.A. Wilderer and M. Wagner. 2001. Nitrification in sequencing biofilm batch reactors: lessons from molecular approaches. Water Sci. Technol. 43: 9–18.
• Dionisi H.M., A.C. Layton, G. Harms, I.R. Gregory, K.G. Robinson and G.S. Sayler. 2002. Quantification of Nitrosomonas oligotropha-like ammonia-oxidizing bacteria and Nitrospira spp. From full-scale wastewater treatment plants by competitive PCR. Appl. Environ. Microbiol. 68: 245–253.
• Dolzani L., E. Tonin, C. Lagatolla, L. Prandin and C. Monti-Bragadin. 1995. Identification of Acinetobacter isolates in the A. calcoaceticus-A. baumannii complex by restriction analysis of the 16S–23S rRNA intergenic-spacer sequences. J. Clin. Microbiol. 33: 1108–1113.
• Ebie Y., M. Matsumura, N. Noda, S. Tsuneda, A. Hirata and Y. Inamori. 2002. Community analysis of nitrifying bacteria in an advanced and compact Gappei-Johkasou by FISH and PCR-DGGE. Water Sci. Technol. 46: 105–111.
• Focht D.D. and W. Verstraete. 1977. Biochemical ecology of nitrification and denitrification. Adv. Microb. Ecol. 1: 135–214.
• Gieseke A., U. Purkhold, M. Wagner, R. Amann and A. Schramm. 2001. Community structure and activity dynamics of nitrifying bacteria in a phosphate-removing biofilm. Appl. Environ. Microbiol. 67: 1351–1362.
• Hall S.J., J. Keller and L.L. Blackall. 2003. Microbial quantification in activated sludge: the hits and misses. Water Sci. Technol. 48: 121–126.
• Juretschko S., A. Lo, A. Lehner and M. Wagner. 2002. the microbial community composition of a nitrifying-denitrifying activated sludge from an industrial sewage treatment plant analyzed by the full-cycle 16S rRNA approach. Syst. Appl. Microbiol. 25: 84–99.
• Kuo D.H.W., K.G. Robinson, A.C. Layton, A.J. Meyers and G.S. Sayler. 2006. Real-time PCR quantification of ammonia-oxidizing bacteria (AOB): solids retention time (SRT) impacts during activated sludge treatment of industrial wastewater. Environ. Eng. Sci. 23(3): 507–520.
• LaPara T.M., C.H. Nakatsu, L.M. Pantea and J.E. Alleman. 2002. Stability of the bacterial communities supported by a seven-stage biological process treating pharmaceutical wastewater as revealed by PCR-DGGE. Water Res. 36: 638–646.
• LaPara T.M. and S. Ghosh. 2006. Population dynamics of the ammonia-oxidizing bacteria in a full-scale municipal wastewater treatment facility. Environ. Eng. Sci. 23: 309–319.
• Lazarova V., D. Bellahcen, D. Rybacki, B. Rittmann and J. Manem. 1998. Population dynamics and biofilm composition in a new threephase circulation bed reactor. Water Sci. Tech. 37: 149–158.
• Limpiyakorn T., F. Kurisu and O. Yagi. 2006. Quantification of ammonia-oxidizing bacteria populations in full-scale sewage activated sludge systems and assessment of system variables affecting their performance. Water Sci. Technol. 54: 91–99.
• Metcalf and Eddy Inc. 1991. Design of Facilities for the Biological Treatment of Wastewater, pp: 529–662. In: Tchobanoglous G. and F.L. Burton (eds.), Wastewater Engineering: Treatment, Disposal and Reuse, 3rd ed., McGraw Hill Inc.
• Mobarry B.K., M. Wagner, V. Urbain, B.E. Rittmann and D.A. Stahl. 1996. Phylogenetic probes for analyzing abundance and spatial organization of nitrifying bacteria. Appl. Environ. Microbiol. 62: 2156–2162.
• Nei M. and W.H. Li. 1979. Mathematical model for studying genetic variation on terms of restriction endonucleases. Proceedings of the National Academy of Sciences 76, USA, pp. 5269.
• Nicolaisen M.H. and N.B. Ramsing. 2002. Denaturing gradient gel electrophoresis (DGGE) approaches to study the diversity of ammonia-oxidizing bacteria. J. Microbiol. Meth. 50: 189–203.
• Nogueira R., L.F. Melo, U. Purkhold, S. Wuertz and M. Wagner. 2002. Nitrifying and heterotrophic population dynamics in biofilm reactors: effects of hydraulic retention time and the presence of organic carbon. Water Res. 36: 469–481.
• Okabe S., H. Satoh and Y. Watanabe. 1999. In situ analysis of nitrifying biofilms as determined by in situ hybridization and the use of microelectrodes. Appl. Environ. Microbiol. 65: 3182–3191.
• Onuki M., H. Satoh, T. Mino and T. Matsuo. 2000. Application of molecular methods to microbial community analysis of activated sludge. Water Sci. Tech. 42: 17–22.
• Rittmann E. and V.L. Snoeyink. 1984. Achieving biologically stable drinking water. J. Am. Water Works Ass. 76: 106–114.
• Rittmann B.E., C.S. Laspidou, J. Flax, D.A. Stahl, V. Urbain, H. Harduin, J.J. van der Waarde, B. Geurkink, M.J.C. Henssen, H. Brouwer and others. 1999. Molecular and modeling analyses of the structure and function of nitrifying activated sludge. Water Sci. Technol. 39: 51–59.
• Rotthauwe J.-H., K.-P. Witzel and W. Liesack. 1997. the ammonia monooxygenase structural gene amoA as a functional marker: molecular fine scale analysis of natural ammonia-oxidizing populations. Appl. Environ. Microbiol. 63: 4704– 4712.
• Rowan A.K., J.R. Snape, D. Fearnside, M.R. Barer, T.P. Curtis and I.M. Head. 2003. Composition and diversity of ammonia-oxidizing bacterial communities in wastewater treatment reactors of different design treating identical wastewater. FEMS Microbiol. Ecol. 43: 195–206.
• Saleem M., A.A. Bukhari and M.H. Al-Malack. 2003. Seasonal variations in the bacterial population in an activated sludge system. J. Environ. Eng. Sci. 2: 155–162.
• S. Siripong and B.E. Rittmann. 2007. Diversity study of nitrifying bacteria in full-scale municipal wastewater treatment plants. Water Res. 41: 1110–1120.
• Stanisz A. 2000. Bases of statistics for science. Ch. 21: Analisis of correlation (in Polish). Med. Praktyczna 10: 176–181.
• Xia S., J. Li and R. Wang. 2008. Nitrogen removal performance and microbial community structure dynamics response to carbonnitrogen ratio in a compact suspended carrier biofilm reactor. Ecol. Eng. 32: 256–262.
• Zhao Y., A. Wang, N. Ren and Y. Zhao. 2008. Microbial community structure in different wastewater treatment processes characterized by single-strand conformation polymorphism (SSCP) technique. Front. Environ. Sci. Eng. China 2: 116–121.
• Internet sources: www.oligo.ibb.waw.pl www.pkn.pl

Uwagi

PL

Rekord w opracowaniu